Efficient derivation of embryonic stem cells from NOD-scid Il2rg −/− mice

Liu, Kang; Fang, Riguo; Li, Haibo; Yang, Weifeng; Miao, Zewei; Wen, Jinhua; Deng, Hongkui

doi:10.1007/s13238-015-0209-6

Cited by 7 publications

(4 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As the efficiency of deriving mouse embryonic stem (ES) cells is highly strain-dependent (McWhir et al, 1996 ; Brook and Gardner, 1997 ), it is important to investigate whether the LCDM condition enables EPS cell derivation from non-permissive strains such as mice with NOD background, which are refractory to ES cell derivation using traditional ES cell culturing medium (Nagafuchi et al, 1999 ; Brook et al, 2003 ). Notably, the efficiency of deriving EPS cells from NOD- scid Il2rg −/− blastocysts was 66.7%, which is significantly higher than previous reports (Liu et al, 2015 ). This result in combination with our previous report supports the notion that the LCDM condition permits EPS cell derivation from different mouse strains, including permissive and non-permissive backgrounds (Yang et al, 2017 ).…”

Section: Discussioncontrasting

confidence: 61%

“…One representative study is the development of a 2i/LIF medium, which supports the maintenance of mouse PSCs in the naïve pluripotent state (Ying et al, 2008 ). Importantly, the use of 2i/LIF medium has enabled successful derivation of mouse PSCs from several non-permissive mouse strains, such as mice with nonobese diabetic (NOD) background (Hanna et al, 2009 ; Nichols et al, 2009 ; Liu et al, 2015 ). However, recent studies have shown that prolonged culture of mouse pluripotent cells in 2i/LIF condition leads to significant impairment of epigenetic and genomic stability as well as of the developmental potential of these cells (Choi et al, 2017 ; Yagi et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Efficient derivation of extended pluripotent stem cells from NOD-scid Il2rg−/− mice

Wang

et al. 2018

Protein Cell

Self Cite

View full text Add to dashboard Cite

Recently we have established a new culture condition enabling the derivation of extended pluripotent stem (EPS) cells, which, compared to conventional pluripotent stem cells, possess superior developmental potential and germline competence. However, it remains unclear whether this condition permits derivation of EPS cells from mouse strains that are refractory or non-permissive to pluripotent cell establishment. Here, we show that EPS cells can be robustly generated from non-permissive NOD-scid Il2rg mice through de novo derivation from blastocysts. Furthermore, these cells can also be efficiently generated by chemical reprogramming from embryonic NOD-scid Il2rg fibroblasts. NOD-scid Il2rg EPS cells can be expanded for more than 20 passages with genomic stability and can be genetically modified through gene targeting. Notably, these cells contribute to both embryonic and extraembryonic lineages in vivo. More importantly, they can produce chimeras and integrate into the E13.5 genital ridge. Our study demonstrates the feasibility of generating EPS cells from refractory mouse strains, which could potentially be a general strategy for deriving mouse pluripotent cells. The generation of NOD-scid Il2rg EPS cell lines permits sophisticated genetic modification in NOD-scid Il2rg mice, which may greatly advance the optimization of humanized mouse models for biomedical applications.

show abstract

Section: Discussioncontrasting

confidence: 61%

Section: Introductionmentioning

confidence: 99%

Efficient derivation of extended pluripotent stem cells from NOD-scid Il2rg−/− mice

Wang

et al. 2018

Protein Cell

Self Cite

View full text Add to dashboard Cite

show abstract

“…[27][28][29] After 7 days, the mice were treated with IRAK inhibitor (10 mg/kg), ABT-737 (40 mg/kg), IRAK inhibitor and ABT-737 combined solution (10 mg/kg IRAK inhibitor and 40 mg/kg ABT-737), IRAK/ABT-NP (10 mg/kg IRAK inhibitor and 40 mg/kg ABT-737), or PBS as control, two times every week. 5 After 4 weeks, peripheral blood samples from each treated group of mice were taken for counting white blood cells (WBC) and staining in accordance with the Wright-Giemsa method.…”

Section: Micementioning

confidence: 99%

Cooperation of IRAK1/4 inhibitor and ABT-737 in nanoparticles for synergistic therapy of T cell acute lymphoblastic leukemia

Wu¹,

Wang²,

Qiu³

et al. 2017

IJN

View full text Add to dashboard Cite

T cell acute lymphoblastic leukemia (T-ALL) is caused by clonal expansion of variant T cell progenitors and is considered as a high risk leukemia. Contemporary single chemotherapy has a limited effect due to dynamic and versatile properties of T-ALL. Here IRAK1/4 inhibitor and ABT-737 were co-encapsulated into polyethylene glycol modified poly (lactic-co-glycolic acid) nanoparticles (IRAK/ABT-NP) to enhance synergistic therapy of T-ALL. The formulation was optimized to achieve high drug loading using Box-Behnken design and response surface methodology. The optimal parameter comprised 2.98% polymer in acetonitrile, a ratio of oil phase to water phase of 1:8.33, and 2.12% emulsifier concentration. High drug loading and uniform spherical shape was achieved. In vitro release study showed sustained release of IRAK1/4 inhibitor for 72 hours as well as sustained release of ABT-737 for more than 120 hours. Uptake efficiency of IRAK/ABT-NP and induced apoptotic T-ALL fraction by IRAK/ABT-NP were much higher than the IRAK1/4 and ABT-737 combined solution. IC 50 of IRAK/ABT-NP was two-fold lower than free drug combination in Jurkat cells. Additionally, we conducted in vivo experiments in which IRAK/ABT-NP exhibited greater cytotoxicity toward T-ALL cells, the capacity to significantly restore white blood cell number in peripheral blood, and improved survival time of T-ALL mouse model compared to the IRAK1/4 and ABT-737 combined solution.

show abstract

“…The addition of small‐molecule inhibitors of the key differentiation pathways to the culture media has been shown to efficiently promote the derivation of ES cells of genetic backgrounds when attempts in serum‐containing medium have failed (Czechanski et al, ) such as NOD (Nichols et al, ), and NSG (Landel, Dunlap, Patton, & Manser, ; Liu et al, ). To establish NRG ES cell lines, we followed a simplified version of the protocol published by Nichols (Nagy & Nichols, ) omitting the zona pellucida removal and the immunosurgery.…”

Section: Resultsmentioning

confidence: 99%

A new genetic tool to improve immune‐compromised mouse models: Derivation and CRISPR/Cas9‐mediated targeting of NRG embryonic stem cell lines

Martín‐González

Baudet

Abelechian

et al. 2018

Genesis

View full text Add to dashboard Cite

Development of human hematopoietic stem cells and differentiation of embryonic stem (ES) cells/induced pluripotent stem (iPS) cells to hematopoietic stem cells are poorly understood. NOD (Non-obese diabetic)-derived mouse strains, such as NSG (NOD-Scid-il2Rg) or NRG (NOD-Rag1-il2Rg), are the best available models for studying the function of fetal and adult human hematopoietic cells as well as ES/iPS cell-derived hematopoietic stem cells. Unfortunately, engraftment of human hematopoietic stem cells is very variable in these models. Introduction of additional permissive mutations into these complex genetic backgrounds of the NRG/NSG mice by natural breeding is a very demanding task in terms of time and resources. Specifically, since the genetic elements defining the NSG/NRG phenotypes have not yet been fully characterized, intense backcrossing is required to ensure transmission of the full phenotype. Here we describe the derivation of embryonic stem cell (ESC) lines from NRG pre-implantation embryos generated by in vitro fertilization followed by the CRISPR/CAS9 targeting of the Gata-2 locus. After injection into morula stage embryos, cells from three tested lines gave rise to chimeric adult mice showing high contribution of the ESCs (70%-100%), assessed by coat color. Moreover, these lines have been successfully targeted using Cas9/CRISPR technology, and the mutant cells have been shown to remain germ line competent. Therefore, these new NRG ESC lines combined with genome editing nucleases bring a powerful genetic tool that facilitates the generation of new NOD-based mouse models with the aim to improve the existing xenograft models.

show abstract

Efficient derivation of embryonic stem cells from NOD-scid Il2rg −/− mice

Cited by 7 publications

References 11 publications

Efficient derivation of extended pluripotent stem cells from NOD-scid Il2rg−/− mice

Efficient derivation of extended pluripotent stem cells from NOD-scid Il2rg−/− mice

Cooperation of IRAK1/4 inhibitor and ABT-737 in nanoparticles for synergistic therapy of T cell acute lymphoblastic leukemia

A new genetic tool to improve immune‐compromised mouse models: Derivation and CRISPR/Cas9‐mediated targeting of NRG embryonic stem cell lines

Contact Info

Product

Resources

About