Conventional embryonic stem cells (ESCs) or induced pluripotent stem cells (iPSCs) derived from primates resemble mouse epiblast stem cells, raising an intriguing question regarding whether the naive pluripotent state resembling mouse embryonic stem cells (mESCs) exists in primates and how to capture it in vitro. Here we identified several specific signaling modulators that are sufficient to generate rhesus monkey fibroblast-derived iPSCs with the features of naive pluripotency in terms of growth properties, gene expression profiles, self-renewal signaling, X-reactivation, and the potential to generate cross-species chimeric embryos. Interestingly, together with recent reports of naive human pluripotent stem cells, our findings suggest several conserved signaling pathways shared with rodents and specific to primates, providing significant insights for acquiring naive pluripotency from other species. In addition, the derivation of rhesus monkey naive iPSCs also provides a valuable cell source for use in preclinical research and disease modeling.

A reader has pointed out that there are duplications of some of the lanes presented in Figure 2B of our originally published manuscript. Upon examination of the affected lanes and the original data, we discovered that there is indeed duplication caused by inadvertent inclusion of erroneous image files during our organization of the published data.In Figure 2B of the original manuscript, the lanes of SALL4 presented are duplications of the lanes of CRIPTO, and the lanes of DNMT3B presented are duplications of the lanes of DPPA2. To correct this figure, we have replaced the indicated lanes with the correct data for SALL4, CRIPTO, DNMT3B, and DPPA2. All panels other than those indicated lanes remain the same. These unintentional mistakes in figure assembly do not affect our underlying data or conclusions. We apologize for any confusion caused by these unfortunate mistakes, and we wish to thank the anonymous reader for bringing them to our attention.The corrected figure is presented below.Cell Stem Cell 16, 211-212, February 5, 2015 ª2015 Elsevier Inc. 211

No abstract