Generation of Naive Induced Pluripotent Stem Cells from Rhesus Monkey Fibroblasts

Abstract

A reader has pointed out that there are duplications of some of the lanes presented in Figure 2B of our originally published manuscript. Upon examination of the affected lanes and the original data, we discovered that there is indeed duplication caused by inadvertent inclusion of erroneous image files during our organization of the published data.In Figure 2B of the original manuscript, the lanes of SALL4 presented are duplications of the lanes of CRIPTO, and the lanes of DNMT3B presented are duplications of the lanes of DPPA2. To correct this figure, we have replaced the indicated lanes with the correct data for SALL4, CRIPTO, DNMT3B, and DPPA2. All panels other than those indicated lanes remain the same. These unintentional mistakes in figure assembly do not affect our underlying data or conclusions. We apologize for any confusion caused by these unfortunate mistakes, and we wish to thank the anonymous reader for bringing them to our attention.The corrected figure is presented below.Cell Stem Cell 16, 211-212, February 5, 2015 ª2015 Elsevier Inc. 211

The pluripotent state in mouse and human

“…Human or non-human primate PSCs with some features of naïve pluripotency have been derived by the conversion of conventional hESCs or induced PSCs (iPSCs) in vitro, through reprogramming of somatic cells to a pluripotent state or by direct derivation from the embryo (Tables 2 and 3) (Buecker et al, 2010;Chan et al, 2013;Fang et al, 2014;Gafni et al, 2013;Hanna et al, 2010;Li et al, 2009;Takashima et al, 2014;Theunissen et al, 2014;Valamehr et al, 2014;Ware et al, 2014. Early studies used exogenous transgenes to trigger the conversion from primed to naïve pluripotency and, in some cases, to stabilise and maintain the naïve state.…”

Generation of Cynomolgus Monkey Chimeric Fetuses using Embryonic Stem Cells

“…Recently, several studies have reported the generation of naive-like human pluripotent stem cells (PSCs) and monkey induced PSCs (iPSCs) by using different combinations of pharmacological agents and cytokines (Hackett and Surani, 2014). Two of these studies reported that growth of PSCs in a media formulation termed NHSM or modified NHSM could yield naive-like human or monkey PSCs with the ability to contribute to interspecies chimeric embryos after their injection into mouse E2.5 morulae (Fang et al, 2014;Gafni et al, 2013). However, it is not known whether primate ESCs have the capacity to generate monkey chimeras.…”

Derivation of Rabbit Embryonic Stem Cells from Vitrified–Thawed Embryos

“…Unexpectedly, it was shown recently that deriving and maintaining naïve human and monkey PSCs require bFGF (Fang et al, 2014;Gafni et al, 2013;Theunissen et al, 2014), which for a long time has been considered as a marker for primed PSCs on the basis of the knowledge gained from mouse and rats. Here, we have demonstrated that bFGF is also indispensable for the derivation as well as the maintenance of rbESCs.…”