Efficient detection of hepatocellular carcinoma by a hybrid blood test of epigenetic and classical protein markers

Iizuka, Norio; Oka, M.; Sakaida, Isao; Moribe, Toyoki; Miura, Toshiaki; Kimura, Naoki; Tamatsukuri, Shigeru; Ishitsuka, Hideo; Uchida, Koichi; Terai, Shuji; Yamashita, Satoyoshi; Okita, Kiwamu; Sakata, Koichiro; Karino, Yoshiyasu; Toyota, Joji; Ando, Eiji; Ide, Tatsuya; Sata, Michio; Tsunedomi, Ryoichi; Tsutsui, Masahito; Iida, Michihisa; Tokuhisa, Yoshihiro; Sakamoto, Kazuhiko; Tamesa, Takao; Fujita, Yusuke; Hamamoto, Yoshihiko

doi:10.1016/j.cca.2010.09.028

Cited by 27 publications

(32 citation statements)

References 32 publications

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“…Forty-nine studies including 14 118 participants assessed the diagnostic accuracy of DCP comparison to AFP, 4,[44][45][46][47][48][49][50][51][52][53][54][55][56][57][58] and 27 studies involving 8927 participants provided data of combination of both markers for detecting HCC. 4,10,12,[14][15][16][17][18][19][20][22][23][24][25][26][28][29][30]32,39,44,45,50,51,53,54,56 Sensitivity estimates for DCP, AFP and combination of both markers ranged from 0.28 to 0.89, 0.08 to 0.86 and 0.48 to 0.94 and the specificities estimates for DCP, AFP and combination of both markers were 0.50 to 1.00, 0.48 to 1.00 and 0.53 to 0.99, respectively ( Fig.…”

Section: Diagnostic Accuracy Of Dcp Afp and Combination Of Both Markmentioning

confidence: 99%

Diagnostic accuracy of des‐gamma‐carboxy prothrombin versus α‐fetoprotein for hepatocellular carcinoma: A systematic review

Zhang

et al. 2013

Hepatology Research

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Section: Diagnostic Accuracy Of Dcp Afp and Combination Of Both Markmentioning

confidence: 99%

Diagnostic accuracy of des‐gamma‐carboxy prothrombin versus α‐fetoprotein for hepatocellular carcinoma: A systematic review

Zhang

et al. 2013

Hepatology Research

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“…DNA methylation is one of the earliest known modification pathways, and a large number of studies have shown that DNA methylation can lead to changes in chromatin structure, DNA conformation, DNA stability and DNA and protein interactions, thus controlling gene expression. Several studies have revealed that alterations in DNA methylation at many genes, including p15 [79], p16 [80], APC [81], SPINT2 [82], SFRP1 [83], p16INK4a [84], TFPI2 [85], GSTP1 [86] and RASSF1A [87, 88], are associated with the initiation and progression of HCC. For example, the Ras association domain family protein 1A (RASSF1A) is a tumor suppressor that is frequently lost in human cancers by promoter-specific methylation.…”

Section: Introductionmentioning

confidence: 99%

Clinical applications of liquid biopsy as prognostic and predictive biomarkers in hepatocellular carcinoma: circulating tumor cells and circulating tumor DNA

Han

et al. 2018

J Exp Clin Cancer Res

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Hepatocellular carcinoma (HCC) is a highly malignant disease with a poor prognosis and high mortality due to a low early diagnosis rate, resistance to systemic treatments and progression to late-stage liver disease. Owing to limitations in the detection of HCC and the lack of awareness of healthcare systems, fewer than 40% of HCC patients are eligible for surgery due to advanced stages of the disease at the time of diagnosis and the occurrence of multiple lesions in the cirrhotic or fibrotic liver. At present, the updated American Association for the Study of Liver Disease (AASLD) guidelines no longer recommend alpha-fetoprotein (AFP) testing as a part of diagnostic evaluation. Thus, it is imperative to establish a novel diagnostic strategy with high sensitivity and reliability to monitor risk factors to detect HCC at an early stage. In recent years, “liquid biopsy,” (including circulating tumor cells (CTCs) and circulating tumor DNA (ctDNA)), has emerged as a technique for the characterization of circulating cells, providing a strong basis for the individualized treatment of patients. As a noninvasive detection method, liquid biopsy is expected to play an important role in the early diagnosis, dynamic monitoring of cancer patients and drug screening. In this review, we will focus on the clinical applications, recent studies and future prospects of liquid biopsy, particularly focusing on HCC.

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“…The protein product of SPINT2 inhibits HGF activator, which prevents the formation of active hepatocyte growth factor, has been taken as a putative tumor suppressor [32]. Previous studies mainly focus on the methylation of SPINT2 in HCC instead of its expression [33, 34]. Nevertheless, we have found that the expression level of SPINT2 was significantly suppressed in HCC expression profiles.…”

Section: Discussionmentioning

confidence: 85%

A transcriptome profile in hepatocellular carcinomas based on integrated analysis of microarray studies

Wang

et al. 2017

Diagn Pathol

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BackgroundDespite new treatment options for hepatocellular carcinomas (HCC) recently, 5-year survival remains poor, ranging from 50 to 70%, which may attribute to the lack of early diagnostic biomarkers. Thus, developing new biomarkers for early diagnosis of HCC, is extremely urgent, aiming to decrease HCC-related deaths.MethodsIn the study, we conducted a comprehensive characterization of gene expression data of HCC based on a bioinformatics method. The results were confirmed by real time polymerase chain reaction (RT-PCR) and TCGA database to prove the credibility of this integrated analysis.ResultsAfter integrating analysis of seven HCC gene expression datasets, 1167 differential expressed genes (DEGs) were identified. These genes mainly participated in the process of cell cycle, oocyte meiosis, and oocyte maturation mediated by progesterone. The results of experiments and TCGA database validation in 10 genes was in full accordance with findings in integrated analysis, indicating the high credibility of our integrated analysis of different gene expression datasets. ASPM, CCT3, and NEK2 was showed to be significantly associated with overall survival of HCC patients in TCGA database.ConclusionThis method of integrated analysis may be a useful tool to minish the heterogeneity of individual microarray, hopefully outputs more accurate HCC transcriptome profiles based on large sample size, and explores some potential biomarkers and therapy targets for HCC.Electronic supplementary materialThe online version of this article (doi:10.1186/s13000-016-0596-x) contains supplementary material, which is available to authorized users.

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Efficient detection of hepatocellular carcinoma by a hybrid blood test of epigenetic and classical protein markers

Cited by 27 publications

References 32 publications

Diagnostic accuracy of des‐gamma‐carboxy prothrombin versus α‐fetoprotein for hepatocellular carcinoma: A systematic review

Diagnostic accuracy of des‐gamma‐carboxy prothrombin versus α‐fetoprotein for hepatocellular carcinoma: A systematic review

Clinical applications of liquid biopsy as prognostic and predictive biomarkers in hepatocellular carcinoma: circulating tumor cells and circulating tumor DNA

A transcriptome profile in hepatocellular carcinomas based on integrated analysis of microarray studies

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