Efficient enzyme encapsulation inside sol-gel silica sheets prepared by poly-_L-lysine as a catalyst

Abstract

High catalytic activities of enzymes are necessary for the enzyme immobilizing technology for the development of better biocatalysts and biosensors. Basic polypeptide (poly-L-lysine)templated precipitation of silica synthesized by sol-gel chemistry produced a composite material that allows high enzyme activity. This study investigates the structural properties of the composite material that allow retaining the glucose oxidase (GOx) activity. Scanning (SEM) and transmission (TEM) electron microscopies reveal that the composite has plate-or sheettype morphologies composed of hexagonal structures that are 0.2-2 µm in diameter. After the encapsulation in a sol-gel silica matrix, encapsulated GOx retained high activity (over 85% of oxidation activity compared with that of free GOx). The relative activity of GOx-encapsulated in SiO 2 @pLys remained approximately at 50-60% after eight cycles; in addition, the catalytic stability of encapsulated GOx improved under high temperature (60°C) and in several solvents (e.g., HCl, urea, and acetone). Moreover, D-glucose detection was performed in the linear range of 1-400 µM using SiO 2 @pLys-GOx composites. The obtained results show that SiO 2 @pLys-GOx composites can be used for enzyme encapsulation with high activity and stability and as biosensor materials with high sensitivity.

Sol-Gel Coatings with Azofoska Fertilizer Deposited onto Pea Seeds

“…In some plant species, silica creates intracellular or extracellular silica bodies (phytolites) which are necessary for growth, mechanical strength, stiffness, and protection against fungi [ 28 ]. Silica prepared under mild conditions using the sol-gel method seem to be ideal for encapsulating fertilizer ingredients, since it is possible to enclose inside them sensitive biological materials such as enzymes [ 29 ], live cells [ 30 ], bacteria [ 31 ], algae [ 32 ], and others [ 33 ]. Encapsulated materials are not damaged.…”

Investigation of poly(lactic acid) nanocapsules containing the plant extract via coaxial electrospraying method for functional nonwoven applications

“…The nano/microencapsulation technique can be the next generation of textile finishing. The encapsulation method has been initially practiced in the biotechnology [20] and has many applications in this area, for instance, enzyme immobilization for biosensors, biocatalysts [21], microorganism encapsulation [22,23], bioactive peptides [24], food formulations, virus capsulation for vaccination, and therapy purposes [25]. Nano/microencapsulation technologies have a wide range of applications in other areas too.…”

“…The nano/microencapsulation technique can be the next generation of textile finishing. The encapsulation method has been initially practiced in the biotechnology [20] and has many applications in this area, for instance, enzyme immobilization for biosensors, biocatalysts [21], microorganism encapsulation [22,23],…”

Functionalizing silica sol-gel with entrapped plant virus-based immunosorbent nanoparticles

“…Extensive literature supports favorable silica sol-gel chemistry characteristics of high structural uniformity, stability, pore size tunability, optical properties, and biodegradability for various biomedical applications 8,9 . A range of live cells [10][11][12] and enzymes [13][14][15] have been studied for bioencapsulation in silica sol-gel matrices to stabilize viability and activity as well as to improve ease of use for the intended application. There have not yet been any such studies using VBNs, with the exception of a study of viral encapsulation focused on extended release of viral vectors for gene therapy 16 .…”