Efficient Heparin Recovery from Porcine Intestinal Mucosa Using Zeolite Imidazolate Framework-8

Abdolmaleki, Mahmood Karimi; Ganta, D.; Shafiee, Ali; Velazquez, Carlo Alberto; Khambhati, Devang P.

doi:10.3390/molecules27051670

Cited by 7 publications

(7 citation statements)

References 40 publications

(58 reference statements)

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“…The highest adsorption efficiency was achieved at the lowest concentration of ammonium chloride. This way, a higher yield of recovered heparin (86.17%) from porcine intestinal mucosa was achieved than in the process using zeolite imidazolate framework-8 (70%) as an adsorbent (Abdolmaleki et al, 2022).…”

Section: Heparin Isolationmentioning

confidence: 96%

“…In the case of adsorption kinetics of heparin onto the zeolite imidazolate framework-8 (Abdolmaleki et al, 2022) or ectoine onto the Dowex HCR-S (Wu et al, 2021), it was described by the pseudo-second-order model better than the pseudo-firstorder model.…”

Section: Kinetic Studymentioning

confidence: 99%

“…The studies on heparin recovery by using resin-based adsorption provided information on isolation and characterization of heparin but scarce in the kinetic data for the adsorption of heparin onto resin. The newly published study of Abdolmaleki et al (2022) has considered the kinetic modeling of heparin adsorption onto zeolite imidazolate framework-8. However, to the best authors' knowledge, the kinetics of heparin adsorption onto Dowex ® 1x1 resin has not been reported.…”

Section: Introductionmentioning

confidence: 99%

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The kinetics of heparin adsorption with Dowex 1x1 ion exchange resin

Milanović

Miladinović

2022

Food Sci. Technol

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The recovery of heparin from the porcine intestinal mucosa by using the ion exchange resin was studied. The procedure involved the following steps: alkaline hydrolysis, ion exchange resin adsorption, and elution. The activity of heparin was measured by an antifactor Xa assay. The ammonium chloride/ammonium hydroxide was used as a buffer, and the effect of salt concentration on the adsorption efficiency was evaluated. The results showed that the highest heparin yield (86.17%) was achieved at the lowest concentration of ammonium chloride. The pseudo-first-order model was shown as more adequate to describe heparin adsorption onto the resin.

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Section: Heparin Isolationmentioning

confidence: 96%

Section: Kinetic Studymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The kinetics of heparin adsorption with Dowex 1x1 ion exchange resin

Milanović

Miladinović

2022

Food Sci. Technol

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“…Besides heparin, this digestion mixture contains nucleic acids, proteins, and other biochemicals. The next step of the heparin extraction process involves several adsorption-desorption steps [7][8][9][10][11][12]. This complex and labor-intensive process makes the extraction unviable and only very low concentrations (0.01% w/w) of heparin are isolated from enzymatically digested natural tissue [13].…”

Section: Introductionmentioning

confidence: 99%

Modified Diatomaceous Earth in Heparin Recovery from Porcine Intestinal Mucosa

Das,

Khambhati,

Longoria

et al. 2023

Molecules

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Heparin, a highly sulfated glycosaminoglycan, is a naturally occurring anticoagulant that plays a vital role in various physiological processes. The remarkable structural complexity of heparin, consisting of repeating disaccharide units, makes it a crucial molecule for the development of commercial drugs in the pharmaceutical industry. Over the past few decades, significant progress has been made in the development of cost-effective adsorbents specifically designed for the adsorption of heparin from porcine intestinal mucosa. This advancement has been driven by the need for efficient and scalable methods to extract heparin from natural sources. In this study, we investigated the use of cationic ammonium-functionalized diatomaceous earth, featuring enhanced porosity, larger surface area, and higher thermal stability, to maximize the isolated heparin recovery. Our results showed that the higher cationic density and less bulky quaternary modified diatomaceous earth (QDADE) could adsorb up to 16.3 mg·g−1 (31%) of heparin from the real mucosa samples. Additionally, we explored the conditions of the adsorbent surface for recovery of the heparin molecule and optimized various factors, such as temperature and pH, to optimize the heparin uptake. This is the introductory account of the implementation of modified diatomaceous earth with quaternary amines for heparin capture.

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“…Despite their advantages, low selectivity toward the recovery of HEP and costly manufacturing processes are the biggest challenges. Up to now, in our group, we have suggested and studied the application of several adsorbents such as ZIF-8, cross-linked spherical polycationic bead, and amine-functionalized carbon/titanium dioxide nanotube arrays on titanium foil for uptake of HEP [ 17 , 18 , 19 ]. These series of studies have demonstrated that having appropriate surface functionality is extremely helpful in increasing the adsorption efficiency of the sorbents.…”

Section: Introductionmentioning

confidence: 99%

Efficient and Economic Heparin Recovery from Porcine Intestinal Mucosa Using Quaternary Ammonium-Functionalized Silica Gel

et al. 2022

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Heparin, usually isolated from porcine intestinal mucosa, is an active pharmaceutical ingredient of great material value. Traditionally, diverse types of commercial resins were employed as an adsorbent for heparin retrieval from biological samples. However, more recent years have encouraged the advent of new cost-effective adsorbents to achieve enhanced heparin retrieval. Inexpensive cationic ammonium-functionalized silica gels, monodispersed with larger surface area, porosity, and higher thermal stability, were chosen to evaluate the heparin recovery yield from porcine intestinal mucosa. We demonstrated that higher positively charged and less bulky quaternary modified silica gel (e.g., QDASi) could adsorb ~28% (14.7 mg g−1) heparin from the real samples. In addition, we also determined suitable surface conditions for the heparin molecule adsorption by mechanistic studies and optimized different variables, such as pH, temperature, etc., to improve the heparin adsorption. This is going to be the first reported study on the usage of quaternary amine-functionalized silica gel for HEP uptake.

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Efficient Heparin Recovery from Porcine Intestinal Mucosa Using Zeolite Imidazolate Framework-8

Cited by 7 publications

References 40 publications

The kinetics of heparin adsorption with Dowex 1x1 ion exchange resin

The kinetics of heparin adsorption with Dowex 1x1 ion exchange resin

Modified Diatomaceous Earth in Heparin Recovery from Porcine Intestinal Mucosa

Efficient and Economic Heparin Recovery from Porcine Intestinal Mucosa Using Quaternary Ammonium-Functionalized Silica Gel

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