Efficient induction of proximity-dependent labelling by biotin feeding in BMAL1-BioID knock-in mice

Murata, Katsuyuki; Mimura, Asuka; Suzuki, Hayate; Mikami, Natsuki; Hamada, Yuko; Kato, Kayoko; Iki, Natsumi; Ishida, Miyuki; Daitoku, Yoko; Tanimoto, Yoko; Okiyoneda, Tsukasa; Fujiyama, Tomoyuki; Dinh, Tra Thi Huong; Mizuno, Seiya; Sugiyama, Fumihiro

doi:10.1093/jb/mvab059

Cited by 9 publications

(7 citation statements)

References 22 publications

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“…Of these 19 studies, 6 were RF studies [18][19][20][21][22][23], 7 involved HFD [24][25][26][27][28][29][30][31], 2 were ketogenic diets [25,32], and 4 were other types of dietary intervention [33][34][35][36], according to Table S1. Table 2 details information about the objectives and methodologies of the selected studies.…”

Section: Literature Datamentioning

confidence: 99%

The Effect of Diet on the Cardiac Circadian Clock in Mice: A Systematic Review

et al. 2022

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Circadian rhythms play important roles in regulating physiological and behavioral processes. These are adjusted by environmental cues, such as diet, which acts by synchronizing or attenuating the circadian rhythms of peripheral clocks, such as the liver, intestine, pancreas, white and brown adipose tissue, lungs, kidneys, as well as the heart. Some studies point to the influence of diet composition, feeding timing, and dietary restriction on metabolic homeostasis and circadian rhythms at various levels. Therefore, this systematic review aimed to discuss studies addressing the effect of diet on the heart clock in animal models and, additionally, the chronodisruption of the clock and its relation to the development of cardiovascular disorders in the last 15 years. A search was conducted in the PubMed, Scopus, and Embase databases. The PRISMA guide was used to construct the article. Nineteen studies met all inclusion and exclusion criteria. In summary, these studies have linked the circadian clock to cardiovascular health and suggested that maintaining a robust circadian system may reduce the risks of cardiometabolic and cardiovascular diseases. The effect of time-of-day-dependent eating on the modulation of circadian rhythms of the cardiac clock and energy homeostasis is notable, among its deleterious effects predominantly in the sleep (light) phase and/or at the end of the active phase.

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Section: Literature Datamentioning

confidence: 99%

The Effect of Diet on the Cardiac Circadian Clock in Mice: A Systematic Review

et al. 2022

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“…When dsDNA donors are used, gene cassette (>1 kb) knockin mice can be generated without laborious donor DNA preparation 7 .…”

Section: Introductionmentioning

confidence: 99%

Zygote Microinjection for Creating Gene Cassette Knock-in and Flox Alleles in Mice

Tanimoto

Mikami

Ishida

et al. 2022

JoVE

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CRISPR-Cas technology has enabled the rapid and effortless generation of genetically modified mice. Specifically, mice and point mutant mice are readily produced by electroporation of CRISPR factors (and single-stranded oligo DNA donors) into the zygote. In contrast, gene cassette (>1 kb) knock-in and floxed mice are mainly generated by microinjection of CRISPR factors and double-stranded DNA donors into zygotes. Genome editing technologies have also increased the flexibility of genetically modified mice production. It is now possible to introduce the intended mutations in the target genomic regions in a number of beneficial inbred mouse strains. Our team has produced over 200 gene cassette knock-in mouse lines, and over 110 floxed mouse lines by zygote microinjection of CRISPR-Cas9 following requests fromseveral countries, including Japan. Some of these genome editing used BALB/c, C3H/HeJ, and C57BL/6N inbred strains, however most used C57BL/6J. Unlike the electroporation method, genome editing by zygote microinjection in various inbred strains of mice is not that easy. However, gene cassette knock-in and floxed mice on single inbred genetic backgrounds are as critical as genetic humanized, fluorescent reporter, and conditional knockout mouse models. Therefore, this article presents the protocol for the zygote microinjection of CRISPR factors and double-stranded DNA donors in C57BL/6J mice for generating gene cassette knock-in and floxed mice. This article exclusively focuses on nuclear injection rather than cytoplasmic injection. In addition to zygote microinjection, we outline the timeline for the production process and peripheral techniques such as induction of superovulation and embryo transfer.

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“…Physiological expression of the fused labelling enzyme could not only prevent artefacts, but at the same time might reveal less abundant interactors that would be hidden in the unspecific labelling background due to overexpression. So far, endogenously expressed biotin ligases have been successfully used in mice and C. elegans [13][14][15][16] , but for cultured cell lines that can be easily transfected, transient overexpression of the labelling enzyme is generally preferred as it presents a faster way to perform the experiments.…”

Section: Introductionmentioning

confidence: 99%

When less is more – A fast TurboID KI approach for high sensitivity endogenous interactome mapping

Stockhammer

Benz

Harel

et al. 2021

Preprint

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In recent years, proximity labelling has established itself as an unbiased and powerful approach to map the interactome of specific proteins. Generally, protein fusions with labelling enzymes are transiently overexpressed to perform these experiments. Using a pipeline for the rapid generation CRISPR-Cas9 knock-ins (KIs) based on antibiotic selection, we were able to compare the performance of commonly used labelling enzymes when endogenously expressed. We found TurboID and its shorter variant miniTurboID to be superior above other labelling enzymes at physiological expression levels. Endogenous tagging of the μ subunit of the AP-1 complex increased the sensitivity for detection of interactors in a proximity labelling experiment and resulted in a more comprehensive mass spectrometry data set. We were able to identify several known interactors of the complex and cargo proteins that simple overexpression of a labelling enzyme fusion protein could not reveal. Our approach greatly simplifies the execution of proximity labelling experiments for proteins in their native cellular environment and allows going from CRISPR transfection to mass spectrometry data in just over a month.

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Efficient induction of proximity-dependent labelling by biotin feeding in BMAL1-BioID knock-in mice

Cited by 9 publications

References 22 publications

The Effect of Diet on the Cardiac Circadian Clock in Mice: A Systematic Review

The Effect of Diet on the Cardiac Circadian Clock in Mice: A Systematic Review

Zygote Microinjection for Creating Gene Cassette Knock-in and Flox Alleles in Mice

When less is more – A fast TurboID KI approach for high sensitivity endogenous interactome mapping

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