Efficient marker-assisted breeding for clubroot resistance in elite Pol-CMS rapeseed varieties by updating the PbBa8.1 locus

Abstract

Clubroot disease poses a severe threat to rapeseed (Brassica napus) production worldwide and has recently been spreading across China at an unprecedented pace. Breeding and cultivation of resistant varieties constitute a promising and environment-friendly approach to mitigating this threat. In this study, the clubroot resistance locus PbBa8.1 was successfully transferred into SC4, a shared paternal line of three elite varieties in ve generations by recurrent selection. Speci c KASP markers of clubroot resistance gene PbBa8.1 and its linked high erucic acid gene (FAE1) were designed and applied for foreground selection, while a 1000 + SNPs were selected and used for the background selection. This breeding strategy resulted recombinants had the highest recovery ratio of the recurrent parent genome (> 95%) at BC 2 F 2, while breaking the linkage with FAE1 during the selection. An updated version of the paternal line (SC4R) was generated at BC 2 F 3 , showing signi cantly improved clubroot resistance at seedling stage by arti cial inoculation and was comparable to that of the donor parent. Field trials of the three elite varieties and their updated versions in ve environments indicated similar agronomic appearance and nal yield. The introduced breeding strategy precisely pyramiding the PbBa8.1 and FAE1 loci with the assistants of technical markers in a shorter period and could be applied to other desirable traits for directional improvement in the future.

Role of BjuA03.BNT1 in enhancing clubroot resistance in resynthesized Brassica juncea L.

“…Hence, breeding resistant varieties stands as the most effective strategy. Therefore, exploring resistance genes to clubroot disease and developing resistant germplasms assumes pivotal importance (Guo et al 2022). In this study, the clubroot-resistant B.jucu CT19, which is derived from the crossing between clubroot-resistant cabbage and black mustard, was used as the foundation to create germplasm for breeding clubroot-resistant varieties.…”

“…Clubroot disease, caused by the soilborne pathogen Plasmodiophora brassicae, is one of the most devastating diseases of cruciferous crops, including Brassica napus, Brassica juncea, Brassica oleracea and Brassica rapa (Guo et al 2022). This disease has led to substantial economic losses on a global scale, impacting regions across Asia, Europe, Australia, and the Americas (Vañó et al 2023).…”

The protist ubiquitin ligase effector PbE3-2 targets RD21A to impede plant immunity

“…This disease is now found in all Brassica-growing areas of the world and seems to be getting worse everywhere, which is notoriously known as “the killer” of rapeseed. Breeding and the cultivation of resistant varieties are the most effective approach to mitigate the threats of this disease (Guo et al , 2022). However, new virulent pathotypes of P. brassicae have constantly emerged (Galindo-Gonzalez et al, 2020).…”

“…Breeding and the cultivation of resistant varieties are the most effective approach to mitigate the threats of this disease (Guo et al, 2022). However, new virulent pathotypes of P. brassicae have constantly emerged (Galindo-Gonzalez et al, 2020).…”