Efficient microtuber production of potato in modified nutrient spray bioreactor system

Rahman, Md. Abdur; Islam, S. M. Shahinul; Chowdhury, Arfatun Nahar; Subramaniam, Sreeramanan

doi:10.1016/j.scienta.2015.06.014

Cited by 20 publications

(10 citation statements)

References 32 publications

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“…Bioreactor is an advanced system for in vitro mass production of quality potato seed and plant materials but microbial contamination is a major problem with this system and it is one of the most prevalent causes of culture and economic loss (Rahman et al, 2015). However, the importance of contamination has been reported by Cassels (1990), Guan et al (2005) and Sharaf-eldin and Weathers (2006).…”

Section: Resultsmentioning

confidence: 99%

“…With this technology a large number of quality plantlets can be produced within a short period (Naik and Karihaloo, 2007). For commercial production of potato microtubers through in vitro culture, bioreactor system is an important and advanced method (Rahman et al, 2015). By this time different bioreactor systems have been developed such as RITA™ (Recipient with ATMautomatized temporary immersion), BCB-Bubble Column Bioreactor, BTBB-Balloon Type Bubble Bioreactor (Takayama, 1991;Alvard, et al, 1993;Paek, et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

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Identification and prevention of microbial contaminants of potato culture in temporary immersion bioreactor (TIB) system

Rahman¹,

Islam²,

Chowdhury³

et al. 2017

MJM

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Aims: Temporary Immersion Bioreactor (TIB) system is an advanced technology for commercial mass production of potato microtubers. Despite of several advantages, this system possess a great risk of culture loss at any stage of micropropagation due to microbial contamination. The aims of this study were to identify microbial contaminants isolated during potato shoot growth in the TIB system, evaluate the efficacy of antimicrobial agents to prevent them, to investigate the effect of those agents in vitro on growth and morphology of potato plantlets. Methodology and results: Six bacteria namely Pseudomonas, Staphylococcus, Klebsiella, Corynebacterium, Proteus, Bacillus and five fungi Aspergillus, Penicillium, Mucor, Fusarium and Rhizopus were isolated from the TIB system. We examined the effect of three antibacterial (Gentamycin, Vancomycin and Tetracycline) and four antifungal agents (Mencozeb, Propiconazole, Bavistin and Copper oxychloride) on the contaminants and on potato shoot growth. Results show that Gentamycin (50 mg/L) and Propiconazole (0.15%) were most effective against the isolated bacteria (35 mm inhibition zone) and fungi (100%) respectively, whereas Gentamycin in combination with Bavistin showed better performance on potato shoot and root development. Conclusion, significance and impact of study: Present study will provide useful guidelines to reduce or eliminate the risk of contamination during micropropagation.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Identification and prevention of microbial contaminants of potato culture in temporary immersion bioreactor (TIB) system

Rahman¹,

Islam²,

Chowdhury³

et al. 2017

MJM

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“…This protocol consists of adding 6benzylaminopurine, chlorocholine chloride and sucrose to the liquid culture medium. Tuberization can also be achieved in bioreactor systems, even resulting in greater yields and less hyperhydricity rates compared to the liquid culture (RAHMAN et al, 2015). The in vitro microtuber, subjected to proper stimuli, can further be directly seeded in open field (SHIN et al, 2018).…”

Section: Tuberizationmentioning

confidence: 99%

Application of tissue culture techniques in potato

Morais¹,

Asmar²,

Silva³

et al. 2018

Biosci. J.

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Potato is the world's most important non-cereal food crop, and therefore, it is considered one of the major food sources for humankind. Its conventional propagation is asexual, by using the tuber, which allows the accumulation and dissemination of pathogens to new cultivation areas. This fact not only impairs the yield of this solanaceous plant, but also threatens the maintenance of genotypes for commercial or breeding purposes. Due to the impossibility of using botanical seed, conservation and exchange of germplasm of this species by means of conventional methods are not feasible. In all potato-producing regions, the demand for high-quality tubers has been paramount to ensure crops production. Thus, biotechnological techniques based on tissue culture are very important. Plant tissue culture offers alternative methods of propagation by in vitro techniques that provide production and multiplication of material with high sanity. Thus, this literature review summarizes the history and current situation of tissue culture techniques applied to potato crop. Besides clonal multiplication, this biotechnological tool makes available initial indexed material to breeding programs and certified seed potato, and facilitates the exchange and conservation of germplasm. For all these reasons, the use of these techniques in potato production chain directly benefits producers by providing high-quality propagules.

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“…Steward et al (1958) described the asexual embryogenesis (somatic embryos) in carrot, which provided a powerful technique for the mass production of artificial embryos. There are some reports on plant regeneration via somatic embryogenesis in various crop plants (Mamun et al 2002, Neto et al 2003, Paul et al 2013, Rahman et al 2015, Saha et al 2017. Many workers have emphasized somatic embryogenesis as a preferred method for genetic improvement and multiplication of valuable germplasm of a number of woody perennials (Gupta and Durzan 1987, Bhansali 1990, Islam and Bhattacharjee 2015.…”

Section: Introductionmentioning

confidence: 99%

An efficient regeneration system through in vitro somatic embryogenesis of barley (Hordeum vulgare L.)

Haque¹,

Islam²

2019

J bio-sci.

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This study was carried out to improve an efficient protocol for in vitro callus induction and plant regeneration using Bangladeshi barley genotypes collected from BARI, Gazipur, Bangladesh. After sterilization embryos were separated carefully from mature seeds of six barley genotypes (BB-1, BB-2, BB-3, BB-4, BB-5 and BB-6) and cultured them in MS medium supplemented with various concentration and combination of PGRs for callus induction and regeneration. Out of six genotypes BB-6 showed highest (38.17%) callus induction in MS + 4.0 mg/l 2,4-D + 200 mg/l L-proline + 300 mg/l casein hydrolysate; whereas, BB-4 and BB-5 showed no callus induction in the same medium. For plant regeneration from embryogenic calli the same genotype (BB-6) also performed the best results (19.25%) in MS medium supplemented with 1.5 mg/l BAP + 30 g/l sucrose. Analysis of variance (ANOVA) showed highly significant differences among the media and the genotypes. J. bio-sci. 27: 89-99, 2019

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Efficient microtuber production of potato in modified nutrient spray bioreactor system

Cited by 20 publications

References 32 publications

Identification and prevention of microbial contaminants of potato culture in temporary immersion bioreactor (TIB) system

Identification and prevention of microbial contaminants of potato culture in temporary immersion bioreactor (TIB) system

Application of tissue culture techniques in potato

An efficient regeneration system through in vitro somatic embryogenesis of barley (Hordeum vulgare L.)

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