Application of tissue culture techniques in potato

Abstract

Potato is the world's most important non-cereal food crop, and therefore, it is considered one of the major food sources for humankind. Its conventional propagation is asexual, by using the tuber, which allows the accumulation and dissemination of pathogens to new cultivation areas. This fact not only impairs the yield of this solanaceous plant, but also threatens the maintenance of genotypes for commercial or breeding purposes. Due to the impossibility of using botanical seed, conservation and exchange of germplasm of this species by means of conventional methods are not feasible. In all potato-producing regions, the demand for high-quality tubers has been paramount to ensure crops production. Thus, biotechnological techniques based on tissue culture are very important. Plant tissue culture offers alternative methods of propagation by in vitro techniques that provide production and multiplication of material with high sanity. Thus, this literature review summarizes the history and current situation of tissue culture techniques applied to potato crop. Besides clonal multiplication, this biotechnological tool makes available initial indexed material to breeding programs and certified seed potato, and facilitates the exchange and conservation of germplasm. For all these reasons, the use of these techniques in potato production chain directly benefits producers by providing high-quality propagules.

Different Plant Growth Regulators on Improvement of Potato (Solanum tuberosum L.)

“…Potato (Solanum tuberosum L.) is one of the significant industrial crops all over the world used for multipurposes. Researchers are always interested in improvement of tissue culture techniques of potato to increase high-quality pathogen-free plantlets production with consistent genetic and physiological traits that are highly efficient in terms of photosynthesis (Pruski, 2007;Mohapatra and Batra, 2017;de Morais et al, 2018). In vitro techniques could also be employed to stimulate the strength and improvement of potato breeding programs and to overcome negative effects related to conventional breeding methods (Tazeb, 2017).…”

Tuber Characteristics of Disease Free Meristem Clones of Potato Genotypes

“…Since virus disease could be prevented by chemically meristem culture system appear to be a suitable approach to obtain virus free plants (Loebenstein et al, 2001;Danci et al, 2012). Morais et al (2018) reviewed the usage of meristem culture in preservation of potato genetic stocks. Sarkar and Naik (1998) studied the effects of incubation and culture media in the in vitro preservation of four potato genotypes.…”

Effect of agar concentration in culture medium on in vitro development of potato plants

“…Therefore, the propagating material needs periodic renewal, as it may suffer degeneration, i.e., cumulative contamination from viruses, fungal and bacterial diseases (Al-Hussaini et al, 2015;Pereira & Fortes, 2003). Tissue culture is an alternative to obtaining high health seeds, the technique consists of aseptically cultivating cells, tissues or organ fragments of a given plant in artificial culture medium under a controlled environment, aiming at the development of new plants (Morais et al, 2018). This cultivation method is characterized by speed and a high rate of multiplication in a short time (Mohapatra & Batra, 2017).…”