Efficient mutation induction using heavy-ion beam irradiation and simple genomic screening with random primers in taro (Colocasia esculenta L. Schott)

Matsuyama, Tomoki; Watanabe, Masao; Murota, Yuri; Nakata, Nanako; Kitamura, Hisashi; Shimokawa, Tetsuya; Ebisuzaki, Toshikazu; Wada, Satoshi; Sato, Shusei; Tabata, Satoshi

doi:10.1016/j.scienta.2020.109568

Cited by 8 publications

(2 citation statements)

References 12 publications

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“…RAPD is the simplest one of all molecular markers and has the advantages of simple operation, low cost, and less DNA consumption. As previously reported, RAPD technology has been used in many plants, such as D. tenuifolia, nger millet (Eleusine coracana L. Gaertn), rice, and taro (Colocasia esculenta L. Schott) [21][22][23][24]. SSR markers reveal polymorphic DNA fragments depending on the difference in the number of microsatellite repeats and are particularly suitable for detecting genetic diversity among closely related cultivars [25,26].…”

Section: Introductionmentioning

confidence: 99%

Efficient mutation induction using carbon-ion beams irradiation and simple genomic screening with SSR and RAPD in japonica rice

Wang

Yang

et al. 2022

Preprint

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Background Carbon ion-beam irradiation has been widely used to advance crop breeding. The purpose of this study was to explore whether irradiation is suitable for mutation creation of japonica rice (Oryza sativaL.) in northeast China and its potential effects on local germplasm resources. Methods and Results 200 Gy irradiation was applied to screened stable rice mutants, random amplified polymorphic DNA (RAPD) and simple sequence repeat (SSR) molecular markers were applied to detect the DNA polymorphisms of mutants and local varieties. Here, the mutants with a shorter maturation period than the mutagenic parent were screened. Among control (Tonghe899) and mutants, RAPD and SSR primers revealed that a total of 574 bands of which 385 were polymorphic (67.07%), all mutants had polymorphic DNA bands, and the polymorphism information content (PIC) of RAPD and SSR varied from 0.500 to 0.924 and 0.836 to 0.954, respectively. Meanwhile, among mutant and other local varieties, RAPD and SSR primers generated a total of 658 amplified bands with 530 polymorphic bands (80.55%). Notably, the addition of mutants reduced the lowest Jaccard’s similarity coefficient of the local varieties population from 0.65 to 0.62. Conclusions In summary, carbon-ion beam irradiating rice seeds generate mutants that can develop as new cultivars, and it slightly expands the genetic diversity of the selected japonica rice from northeast China. RAPD and SSR markers had good polymorphism and could be used for DNA polymorphism identification and facilitate inter-cultivar identification for japonica rice in northeast China.

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Section: Introductionmentioning

confidence: 99%

Efficient mutation induction using carbon-ion beams irradiation and simple genomic screening with SSR and RAPD in japonica rice

Wang

Yang

et al. 2022

Preprint

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“…Ion beam technology was developed in Japan and is characterized by high mutagenesis efficiency, even at low doses, with minimal adverse effects on growth because it can deliver higher amounts of energy more locally than X-rays and γ-rays [ 6 ], which have been used for conventional mutagenesis [ 7 , 8 ]. There are many examples of mutants created by ion beam irradiation in plants, such as torenia [ 9 , 10 ], verbena [ 11 , 12 ], gentian [ 13 ], cyclamen [ 14 ], petunia [ 15 ], tricyrtis hirta [ 16 ], carnation [ 17 , 18 ], and Colocasia [ 19 ]. And the most common case of mutant production by ion beam irradiation is in chrysanthemum [ 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 ], which are in the same family as gerbera, but it has not been reported in gerbera.…”

Section: Introductionmentioning

confidence: 99%

Effect of Three Types of Ion Beam Irradiation on Gerbera (Gerbera hybrida) In Vitro Shoots with Mutagenesis Efficiency

Hosoguchi

Uchiyama

Komazawa

et al. 2021

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Gerbera in vitro shoots were irradiated using three types of ion beams with different line energy transfers (LETs) to investigate the effective LET and absorbed doses for mutagenesis. Furthermore, genomic mutation analyses were conducted on the obtained mutants. Survival rate analysis showed a lower lethal dose 50% (LD50) with ion beams with higher LETs. Trait/morphological mutations exhibited changes in the color and shape of petals and male sterility. Irradiation conditions with the highest growth change and trait/morphological mutation rates in each ion were C irradiation at 10 Gy, Ar irradiation at 5 Gy, and Fe irradiation at 5 Gy, with a range of absorbed dose of around LD50 to about 10 Gy lower. The highest trait/morphological mutation rate was 14.1% with Ar irradiation at 5 Gy, which was one of the criteria for ion beam irradiation of gerbera in vitro shoots. Furthermore, the genomic mutation in the flower color, petal shape, and male sterile mutants were confirmed by genotype analysis using Genotyping by Random Amplicon Sequencing-Direct technology. This is the first study to report the efficient production of gerbera mutants that could be analyzed. Our findings may lead to more efficient gerbera mutant production and analysis technology.

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Random mutagenesis in vegetatively propagated crops: opportunities, challenges and genome editing prospects

et al. 2021

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Efficient mutation induction using heavy-ion beam irradiation and simple genomic screening with random primers in taro (Colocasia esculenta L. Schott)

Cited by 8 publications

References 12 publications

Efficient mutation induction using carbon-ion beams irradiation and simple genomic screening with SSR and RAPD in japonica rice

Efficient mutation induction using carbon-ion beams irradiation and simple genomic screening with SSR and RAPD in japonica rice

Effect of Three Types of Ion Beam Irradiation on Gerbera (Gerbera hybrida) In Vitro Shoots with Mutagenesis Efficiency

Random mutagenesis in vegetatively propagated crops: opportunities, challenges and genome editing prospects

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