Efficient oleaginous yeasts for lipid production from lignocellulosic sugars and effects of lignocellulose degradation compounds on growth and lipid production

Poontawee, Rujiralai; Yongmanitchai, Wichien; Limtong, Savitree

doi:10.1016/j.procbio.2016.11.013

Cited by 97 publications

(56 citation statements)

References 65 publications

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“…There are certain criteria that these strains should meet, such as the ability to grow to high cell densities along with high lipid content on various carbon sources and robust process conditions [79][80][81][82]. To improve economic feasibility, oleaginous yeast strains have thus been cultivated on various non-food competing carbon sources, such as lignocellulosic materials [42,44,74,[83][84][85][86]. The non-oleaginous yeast Saccharomyces cerevisiae is used in many industrial applications since it is easy to cultivate and its genetic tools are well-established.…”

Section: Oleaginous Yeast and Filamentous Fungimentioning

confidence: 99%

An Overview of Potential Oleaginous Microorganisms and Their Role in Biodiesel and Omega-3 Fatty Acid-Based Industries

et al. 2020

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Microorganisms are known to be natural oil producers in their cellular compartments. Microorganisms that accumulate more than 20% w/w of lipids on a cell dry weight basis are considered as oleaginous microorganisms. These are capable of synthesizing vast majority of fatty acids from short hydrocarbonated chain (C6) to long hydrocarbonated chain (C36), which may be saturated (SFA), monounsaturated (MUFA), or polyunsaturated fatty acids (PUFA), depending on the presence and number of double bonds in hydrocarbonated chains. Depending on the fatty acid profile, the oils obtained from oleaginous microorganisms are utilized as feedstock for either biodiesel production or as nutraceuticals. Mainly microalgae, bacteria, and yeasts are involved in the production of biodiesel, whereas thraustochytrids, fungi, and some of the microalgae are well known to be producers of very long-chain PUFA (omega-3 fatty acids). In this review article, the type of oleaginous microorganisms and their expertise in the field of biodiesel or omega-3 fatty acids, advances in metabolic engineering tools for enhanced lipid accumulation, upstream and downstream processing of lipids, including purification of biodiesel and concentration of omega-3 fatty acids are reviewed.

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Section: Oleaginous Yeast and Filamentous Fungimentioning

confidence: 99%

An Overview of Potential Oleaginous Microorganisms and Their Role in Biodiesel and Omega-3 Fatty Acid-Based Industries

et al. 2020

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“…These values are determined directly by the type of pretreatment used and the source of feedstock. Most of these compounds affect the metabolism of the microorganisms during fermentation, negatively impacting the yield . Table shows the effect of inhibitors on biomass accumulation and lipid production in three different oleaginous yeast.…”

Section: Inhibitors Effect In Fatty Acids/alkanes Production By Rhodomentioning

confidence: 99%

Challenges in lipid production from lignocellulosic biomass using Rhodosporidium sp.; A look at the role of lignocellulosic inhibitors

Osorio‐González

Hegde

Brar

et al. 2018

Biofuels Bioprod Bioref

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The use of lignocellulosic biomass biofuels is an attractive alternative because they do not put food safety at risk, they are a renewable source, and their use is limited. The use of microorganisms has now become more widespread to take advantage of the carbohydrates present in this raw material (cellulose and hemicellulose) and the products of its hydrolysis (glucose, xylose, arabinose, galactose, and mannose). The fatty acids obtained from oleaginous microorganisms are potential sources for the production of drop‐in biofuels. Rhodosporidium sp. is an oleaginous yeast that accumulates up to 70% of its biomass in the form of lipids and is highly adaptable to different types of substrates. This review discusses the different factors and challenges (genetic modification of strains, pretreatments, and inhibitor effects) in obtaining lipid from lignocellulosic biomass using Rhodosporidium sp. © 2018 Society of Chemical Industry and John Wiley & Sons, Ltd

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“…Aspergillus terreus, Mortierella alpina, and M. isabellina, were reported to produce special lipids such as gamma-linolenic acid (GLA), arachidonic acid (ARA), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA) [6,7]. Among such microorganisms, yeasts have been frequently applied in microbial lipid production because their characteristics are superior to those of other microorganisms: They offer high growth rates, high cellular lipid contents, and low risk of contamination that is easy to scale-up [8,9]. Moreover, the yeast cultivation technology for high cell and lipid content is well-developed [1,8,9] and the fatty acid composition of yeast lipids can be manipulated by modifying the culture conditions [10].…”

Section: Introductionmentioning

confidence: 99%

Feeding Strategies of Two-Stage Fed-Batch Cultivation Processes for Microbial Lipid Production from Sugarcane Top Hydrolysate and Crude Glycerol by the Oleaginous Red Yeast Rhodosporidiobolus fluvialis

Poontawee

2020

Microorganisms

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Microbial lipids are able to produce from various raw materials including lignocellulosic biomass by the effective oleaginous microorganisms using different cultivation processes. This study aimed to enhance microbial lipid production from the low-cost substrates namely sugarcane top hydrolysate and crude glycerol by Rhodosporidiobolus fluvialis DMKU-SP314, using two-stage fed-batch cultivation with different feeding strategies in a 3 L stirred-tank fermenter. The effect of two feeding strategies of 147.5 g/L crude glycerol solution was evaluated including pulse feeding at different starting time points (48, 24, and 72 h after initiation of batch operation) and constant feeding at different dilution rates (0.012, 0.020, and 0.033 h−1). The maximum lipid concentration of 23.6 g/L and cell mass of 38.5 g/L were achieved when constant feeding was performed at the dilution rate of 0.012 h−1 after 48 h of batch operation, which represented 1.24-fold and 1.27-fold improvements in the lipid and cell mass concentration, respectively. Whereas, batch cultivation provided 19.1 g/L of lipids and 30.3 g/L of cell mass. The overall lipid productivity increased to 98.4 mg/L/d in the two-stage fed-batch cultivation. This demonstrated that the two-stage fed-batch cultivation with constant feeding strategy has the possibility to apply for large-scale production of lipids by yeast.

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Efficient oleaginous yeasts for lipid production from lignocellulosic sugars and effects of lignocellulose degradation compounds on growth and lipid production

Cited by 97 publications

References 65 publications

An Overview of Potential Oleaginous Microorganisms and Their Role in Biodiesel and Omega-3 Fatty Acid-Based Industries

An Overview of Potential Oleaginous Microorganisms and Their Role in Biodiesel and Omega-3 Fatty Acid-Based Industries

Challenges in lipid production from lignocellulosic biomass using Rhodosporidium sp.; A look at the role of lignocellulosic inhibitors

Feeding Strategies of Two-Stage Fed-Batch Cultivation Processes for Microbial Lipid Production from Sugarcane Top Hydrolysate and Crude Glycerol by the Oleaginous Red Yeast Rhodosporidiobolus fluvialis

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