Efficient osteogenic differentiation of the dental pulp stem cells on β‐glycerophosphate loaded polycaprolactone/polyethylene oxide blend nanofibers

Abstract

Hard tissue lesion treatment in oral and maxillofacial has been challenging because of tissue complexities. This study aimed to investigate novel biopolymeric construct effects on the osteogenic differentiation potential of the dental pulp stem cells (DPSCs) for introducing a cell copolymer bioimplant. A blended polycaprolactone (PCL)‐polyethylene oxide (PEO) was fabricated using electrospinning, simultaneously filled by β‐glycerophosphate (β‐GP). After that biocompatibility and release kinetics of the PCL‐PEO+β‐GP was evaluated and compared with PCL‐PEO and then the osteogenic differentiation potential of the DPSCs was examined while being cultured on the scaffolds and compared with those cultured on the culture plate. The results demonstrated that scaffolds have not any cytotoxicity and β‐GP can release in a long‐term manner. Alkaline phosphatase activity and calcium content were significantly increased in DPSCs while being cultured on the PCL‐PEO+β‐GP compared with the other groups. Runt‐related transcription factor 2, collagen type‐I, osteonectin, and osteocalcin (OSC) genes expression was upregulated in DPSCs cultured on the PCL‐PEO+β‐GP and was significantly higher than those cultured on the PCL‐PEO. Immunocytochemistry result also confirmed the positive effects of PCL‐PEO+β‐GP on the osteogenic differentiation of the DPSCs by presenting a higher OSC protein expression. According to the results, incorporation of the β‐GP in PCL‐PEO makes a better construct for osteogenic induction into the stem cells and it could be also considered as a great promising candidate for bone, oral, and maxillofacial tissue engineering applications.

Nanofibrous asymmetric collagen/curcumin membrane containing aspirin-loaded PLGA nanoparticles for guided bone regeneration

“…The profile of gene expression of the DPSCs, which were seeded on the membranes control group in the osteogenic media, was consistent with the results of other studies 73,74 . Our findings showed that the new membranes enhanced the osteogenic properties of the collagenic membranes.…”

Functional Dental Pulp Regeneration: Basic Research and Clinical Translation

“…The development of multipurpose composite polymer scaffolds, such as polyhydroxybutyrate (PHB)/chitosan/bioglass nanofibers [93] and β-glycerophosphate (β-GP)-loaded PCL/polyethylene oxide (PEO) blend nanofibers [94], promotes a paradigm shift of scaffolds from biocompatible cell transporters or simple delivery vehicles to biofunctional and guiding matrices. Further work should focus on assessing the synergetic effects between different polymer compositions and the added active therapeutics and cells.…”

circAKT3 positively regulates osteogenic differentiation of human dental pulp stromal cells via miR-206/CX43 axis

“…However, they can be more easily and non-invasively gained from removed teeth in comparison with BMSCs. Accumulating evidence has proved the potential of DPSCs in generating bone-like tissues and repairing bone loss [6][7][8]. For all these reasons, hDPSCs represent rising candidates for therapies of bone tissue repair.…”