Nanofibrous asymmetric collagen/curcumin membrane containing aspirin-loaded PLGA nanoparticles for guided bone regeneration

Abstract

The goal of the current study was to develop an asymmetric guided bone regeneration (GBR) membrane benefiting from curcumin and aspirin. The membrane was prepared using electrospinning technique and then was physic-chemically characterized by the conventional methods. The release profile of aspirin from the prepared membrane was also measured by ultraviolet spectrophotometry. Also, the antibacterial activities of the membrane was evaluated. We also assessed the in vitro effects of the prepared membrane on the biocompatibility and osteogenic differentiation of dental pulp stem cells (DPSCs), and evaluated in vivo bone regeneration using the prepared membrane in the defects created in both sides of the dog’s jaw by histology. The results from the characterization specified that the membrane was successfully prepared with monodispersed nanosized fibers, uniform network shaped morphology, negative surface charge and sustained release platform for aspirin. The membrane also showed antimicrobial effects against all tested bacteria. The presence of curcumin and aspirin in the asymmetric membrane enhanced osteogenic potential at both transcriptional and translational levels. The results of the animal test showed that the test area was completely filled with new bone after just 28 days, while the commercial membrane area remained empty. There was also a soft tissue layer above the new bone area in the test side. We suggested that the prepared membrane in this work could be used as a GBR membrane to keep soft tissue from occupying bone defects in GBR surgeries. Besides, the surgeries can be benefited from antibacterial activities and bone healing effects of this novel GBR membrane while, simultaneously, promoting bone regeneration.

Dental-Derived Mesenchymal Stem Cells: State of the Art

“…Researchers prepared guided bone regeneration (GBR) membrane with curcumin and aspirin addition. By utilizing this novel GBR membrane and DPSCs, bone regeneration was promoted, and meanwhile, antimicrobial effects were observed (Ghavimi et al, 2020). A similar result was found by another group that aspirin, in tetra-PEG hydrogel-based aspirin-sustained release system, could enhance the osteogenic capacity of PDLSCs (Zhang et al, 2019).…”

Development and biocompatibility of the injectable collagen/nano-hydroxyapatite scaffolds as in situ forming hydrogel for the hard tissue engineering application

“…These motifs provide the suitable binding sites that made the Col choice component for the synthesis of the engineered scaffolds [23,25]. Therefore, the Col is combined with different polymers and biomaterials for hard tissue engineering as well as cartilage, bone, and tooth [26,27]. The collagen-PCL binding occurs through COOH and improves cell binding and growth, which could be explained by the fact that the bioactive molecules improve the collagen characteristics [23].…”

Early Osteogenic Differentiation Stimulation of Dental Pulp Stem Cells by Calcitriol and Curcumin

“…Curcumin, a natural polyphenolic hydrophobic product, is extracted from the rhizomes of Curcuma longa. It has various medicinal properties including antioxidant and anti-inflammatory, antimicrobial, antifungal, antiviral and antiangiogenic, anticancer, and antiatherosclerotic effects [4][5][6][7][8][9][10][11].…”