Efficient plant regeneration of Malaysian aromatic rice (Oryza sativa L.) via improved somatic embryogenesis pathway

Rahman, Zuraida Abdul; Ramli, Asfaliza; Kamaruzaman, Rahiniza; Seman, Zulkifli Ahmad; Othman, Ayu Nazreena; Zainal, Zamri; Uddain, Jasim; Subramaniam, Sreeramanan

doi:10.9755/ejfa.2015-07-535

Cited by 14 publications

(24 citation statements)

References 25 publications

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“…Previous research showed that the recalcitrance of indica rice was attributed to low callusing and regenerating abilities [24] compared to japonica subspecies [22] in particular to various conditions of in vitro tissue culture. Eventually, within indica subspecies, a significant variation was also found with in vitro culture response in different genotypes [3].…”

Section: Introductionmentioning

confidence: 93%

“…Rice (Oryza sativa L.) is the second most widely cultivated crop in the world [1]. The major subspecies of rice are indica and japonica, while indica subspecies is the most widely cultivated crop in south and southeast Asian countries [2,3]. The National Agrofood Policy of Malaysia (2011-2012) highlighted that local rice production should be increased to ensure the country's demand for the growing population.…”

Section: Introductionmentioning

confidence: 99%

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Efficient Callus Induction and Regeneration in Selected Indica Rice

Mostafiz

Wagiran

2018

Agronomy

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An efficient callus induction and in vitro regeneration were developed using plant growth regulators, carbon sources, and basal media for three selected Malaysian wetland rice varieties (MR220, MR220-CL2, and MR232) and one upland variety (Bario). Effect of plant growth regulator (PGR) was carried out using four different concentrations (1-4 mg/L) of 2,4-D (2, 4-dichlorophenoxyacetic acid), and NAA (1-naphthalene acetic acid) (2.5, 5.0, 7.5, and 10 mg/L) with optimized 2,4-D. Effects of carbon sources (maltose and sorbitol), and basal media (MS, N6, and LS) were also studied with optimized PGR to maximize the induction of regenerable calli. This study found that all four varieties exhibited high frequency of callus induction on MS (Murashige and Skoog) medium that was supplemented with 3 mg/L 2,4-D and 30 g/L maltose. Callus induction frequencies in the cases of MR220, MR220-CL2, MR232, and Bario were found to be 76%, 94%, 85%, and 42% respectively. Morphological analysis through scanning electron microscopy (SEM) and histological analysis revealed the embryogenicity of the induced callus. In the regeneration study, it was observed that combination of 2 mg/L BAP (6-benzylaminopurine), 2 mg/L Kin (Kinetin) and 0.5 mg/L NAA supplemented MS medium has the potential to promote regeneration of selected indica rice varieties with higher regeneration percentage, i.e., 82% (MR220-CL2), 68% (both in MR220 and MR232), and 40% (Bario). The optimized conditions for callus formation and regeneration can be useful for biotechnological practices for the genetic improvement of Malaysian indica rice.

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Section: Introductionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Efficient Callus Induction and Regeneration in Selected Indica Rice

Mostafiz

Wagiran

2018

Agronomy

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“…Based on color and structural morphology, the calli of the present study were mostly embryogenic in maltose treated media where they reached up to 38 %. Previous studies illustrated that maltose gave high embryo formation in indica rice [11]. It has been reported that Callus induction is highly genotype specific [4].…”

Section: Discussionmentioning

confidence: 98%

Studies on <i>In Vitro </i>Response to Callus Induction and Regeneration of Five High Yielding <i>Indica </i>rice Varieties

Khan

Islam²,

Islam³

2019

JHPR

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Due to growing population, there is an increasing demand of rice production but the productivity of rice is lessened day by day. To overcome this problem various biotechnological tools can be used for developing various rice varieties. However, the lack of a simple and efficient protocol for callus induction, embryogenic callus formation and quick plant regeneration in this cereal crop. In this study embryogenic calli from mature seeds of five indica rice varieties viz. Binadhan-5, Binadhan-6, BRRI dhan-48, BRRI dhan-58 and IR-64 were observed that is done in four different types of media composition. The highest callus induction were observed in media containing Sucrose as a carbon source. Among those varieties Binadhan-6 and BRRI dhan-48 showed highest rate of callus induction respectively. This study will be useful for selecting suitable callus induction medium for callus induction in future that will be useful for not only national but also international plant breeders for producing new variety and so on.

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“…Somatic embryogenesis is an important plant propagation method used as a tool for crop improvement. The success of somatic embryogenesis depends on various factors including genotype, tissue, explant, cultural conditions and developmental stages of the mother plant (Rahman et al 2010). Callus induction and regeneration are the main steps used for crop plants in biotechnological approaches.…”

Section: Introductionmentioning

confidence: 99%

The Effects of Temperature on Callus Induction and Regeneration in Selected Malaysian Rice Cultivar Indica

Mostafiz¹,

Wagiran²,

Johan³

et al. 2018

JSM

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The development of an efficient tissue culture protocol for somatic embryo would facilitate the genetic modification in breeding program. The present study describes the reproducible protocols for three wetland Malaysian rice cultivars (MR232, MR220 and MR220-CL2) and upland rice (Bario) via somatic embryogenesis. In the present study, four preheat treatments (35, 40, 45 and 50°C) were applied to mature seeds with different imbibition periods (3, 5 and 7 days) prior to culture on MS media with 3 mg/L 2,4-D. The results showed that the cultivars exhibited the highest callus induction percentage from 45°C pre-heated seeds and 3 days imbibition (100%, 96%, 100% and 95% for MR232, MR220, MR220-CL2 and Bario, respectively). Callus was induced early ranging from 3 to 12 days compared to without pre-heat treatment. The regeneration efficiency for MR220 and MR220-CL2 cultivars was significantly higher compared to the control treatment. However, both 45°C and 25°C (control) treatments produced higher plantlet regeneration for MR232 and Bario. This study observed that pre-heat treated seeds prior to callus induction did promote callusing and hence regeneration. These findings can be used to establish a suitable protocol for the in vitro regeneration system for several genetic improvements in the numerous stress tolerances of Malaysian rice.

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Efficient plant regeneration of Malaysian aromatic rice (Oryza sativa L.) via improved somatic embryogenesis pathway

Cited by 14 publications

References 25 publications

Efficient Callus Induction and Regeneration in Selected Indica Rice

Efficient Callus Induction and Regeneration in Selected Indica Rice

Studies on <i>In Vitro </i>Response to Callus Induction and Regeneration of Five High Yielding <i>Indica </i>rice Varieties

The Effects of Temperature on Callus Induction and Regeneration in Selected Malaysian Rice Cultivar Indica

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