Efficient Plant Regeneration with Arabinogalactan-Proteins on Various Ploidy Levels of Cereals

Coşkun, Yasemin; Duran, Ragbet Ezgi; Savaskan, Cigdem; Demırcı, Tunhan; Hakan, Mehmet Tolgahan

doi:10.1016/s2095-3119(13)60242-7

Cited by 7 publications

(6 citation statements)

References 19 publications

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“…Although important advances have been made in the field of Triticeae, a highly efficient and cost-effective regeneration system (similar to those available for rice) using wheat MEs is still not available for all genotypes. Extensive work is currently being conducted in order to improve the understanding and control of the expression of totipotency in wheat, notably in recent years by using zygotic MEs as explants (Jia et al 2007 ; Tang et al 2006 ; Yin et al 2011 ; Yu et al 2008 ; Coskun et al 2013 ; Miroshnichenko et al 2013 ; Murín et al 2012 ; Ozbay and Özgen 2010 ; Parmar et al 2012 ).…”

Section: Discussionmentioning

confidence: 99%

“…This is illustrated by the numerous studies conducted in recent years on optimizing in vitro culture conditions for callus induction, plant regeneration, and transformation parameters of ME-based cultures. Among the many processes and factors being evaluated in order to optimize the overall regeneration capacity of elite local wheat cultivars worldwide are genotype screening and genetic influence, inoculation method and explant treatment, preculture incubation time and conditions, different media with organic/inorganic additives, and varying phytohormone concentrations and combinations throughout successive steps of the morphogenic process (Battal 2010 ; Coskun et al 2013 ; Ding et al 2009 ; Miroshnichenko et al 2009 , 2011 , 2013 ; Moghaieb et al 2010 ; Murín et al 2012 ; Ozbay and Özgen 2010 ; Parmar et al 2012 ; Rashid et al 2009 ; Ren et al 2010 ; Tang et al 2006 ; Yin et al 2011 ; Yu et al 2008 ).…”

Section: Introductionmentioning

confidence: 99%

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Morpho-histology and genotype dependence of in vitro morphogenesis in mature embryo cultures of wheat

et al. 2014

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Cellular totipotency is one of the basic principles of plant biotechnology. Currently, the success of the procedure used to produce transgenic plants is directly proportional to the successful insertion of foreign DNA into the genome of suitable target tissue/cells that are able to regenerate plants. The mature embryo (ME) is increasingly recognized as a valuable explant for developing regenerable cell lines in wheat biotechnology. We have previously developed a regeneration procedure based on fragmented ME in vitro culture. Before we can use this regeneration system as a model for molecular studies of the morphogenic pathway induced in vitro and investigate the functional links between regenerative capacity and transformation receptiveness, some questions need to be answered. Plant regeneration from cultured tissues is genetically controlled. Factors such as age/degree of differentiation and physiological conditions affect the response of explants to culture conditions. Plant regeneration in culture can be achieved through embryogenesis or organogenesis. In this paper, the suitability of ME tissues for tissue culture and the chronological series of morphological data observed at the macroscopic level are documented. Genetic variability at each step of the regeneration process was evaluated through a varietal comparison of several elite wheat cultivars. A detailed histological analysis of the chronological sequence of morphological events during ontogeny was conducted. Compared with cultures of immature zygotic embryos, we found that the embryogenic pathway occurs slightly earlier and is of a different origin in our model. Cytological, physiological, and some biochemical aspects of somatic embryo formation in wheat ME culture are discussed.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Morpho-histology and genotype dependence of in vitro morphogenesis in mature embryo cultures of wheat

et al. 2014

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“…In cereals, the involvement of AGPs in growth and development is poorly documented (Coskun et al 2010(Coskun et al , 2013. It has been reported that addition of AGPs to the culture medium increased green plant regeneration rate in wheat (Letarte et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Arabinogalactan proteins improve plant regeneration in barley (Hordeum vulgare L.) anther culture

Makowska

Kaluzniak

Oleszczuk

et al. 2017

Plant Cell Tiss Organ Cult

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“…Ikizce-96) and durum (cv. Mirzabey) wheat from 77.77% and 72.11%, to 94.86% and 89.73%, respectively [ 65 ]. Kumar et al [ 66 ] were able to achieve close to 100% plant regeneration from calli induced from wheat mature and immature embryos on 2.0 mg/l picloram, using a regeneration medium with 0.1 mg/l 2,4-D, 5.0 mg/l zeatin, and 15 mg/l CuSO 4 .…”

Section: Progress In Wheat Genetic Transformationmentioning

confidence: 99%

Genetic Modification for Wheat Improvement: From Transgenesis to Genome Editing

Borisjuk

Kishchenko

Eliby

et al. 2019

BioMed Research International

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To feed the growing human population, global wheat yields should increase to approximately 5 tonnes per ha from the current 3.3 tonnes by 2050. To reach this goal, existing breeding practices must be complemented with new techniques built upon recent gains from wheat genome sequencing, and the accumulated knowledge of genetic determinants underlying the agricultural traits responsible for crop yield and quality. In this review we primarily focus on the tools and techniques available for accessing gene functions which lead to clear phenotypes in wheat. We provide a view of the development of wheat transformation techniques from a historical perspective, and summarize how techniques have been adapted to obtain gain-of-function phenotypes by gene overexpression, loss-of-function phenotypes by expressing antisense RNAs (RNA interference or RNAi), and most recently the manipulation of gene structure and expression using site-specific nucleases, such as CRISPR/Cas9, for genome editing. The review summarizes recent successes in the application of wheat genetic manipulation to increase yield, improve nutritional and health-promoting qualities in wheat, and enhance the crop’s resistance to various biotic and abiotic stresses.

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Efficient Plant Regeneration with Arabinogalactan-Proteins on Various Ploidy Levels of Cereals

Cited by 7 publications

References 19 publications

Morpho-histology and genotype dependence of in vitro morphogenesis in mature embryo cultures of wheat

Morpho-histology and genotype dependence of in vitro morphogenesis in mature embryo cultures of wheat

Arabinogalactan proteins improve plant regeneration in barley (Hordeum vulgare L.) anther culture

Genetic Modification for Wheat Improvement: From Transgenesis to Genome Editing

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