Efficient Production of 1,3‐Dioleoyl‐2‐Palmitoylglycerol through <i>Rhodococcus opacus</i> Fermentation

Zhang, Lin-Shang; Chu, Mei-Yun; Zong, Min‐Hua; Yang, Juan; Lou, Wen‐Yong

doi:10.1002/aocs.12373

Cited by 14 publications

(8 citation statements)

References 31 publications

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“…The biomass and oil content of the batch cultivation are shown in Figure . The biomass of HMFS V and VI reached 3.02 and 3.35 g/L, respectively, which is consistent with other reports. , The small biomass can be explained by the fact that the batch cultivation was heterogeneous. The oil content determined in this study was 44.90 and 48.15 [%, dry cell weight (DCW)], respectively.…”

Section: Results and Discussionsupporting

confidence: 92%

“…More specifically, R. opacus accumulates saturated fatty acids, especially C16:0, at the sn -2 position. ,, When a suitable carbon source is provided in the medium, HMFS is produced.…”

Section: Results and Discussionmentioning

confidence: 99%

“…Recently, Saccharomyces cerevisiae has been engineered to produce structured lipids, and the relative content of potential cocoa butter-like lipids in the TAG reached up to 22%. Rhodococcus opacus PD630 is an efficient oil producer , and has been used for producing OPO (titer 0.62 g/L) and OPL (titer 0.34–0.67 g/L) . In the present study, HMFS was produced by R.…”

Section: Introductionmentioning

confidence: 99%

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Facile and Green Production of Human Milk Fat Substitute through Rhodococcus opacus Fermentation

Zhang

Chu

Zong

et al. 2020

J. Agric. Food Chem.

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Human milk fat substitute (HMFS) is a class of structured lipids widely used in infant formulas. Herein, HMFS was prepared by Rhodococcus opacus fermentation. The substrate oils suitable for HMFS production were coconut oil (66.1−57.5%), soybean oil (17.5−26.5%), high oleic acid sunflower oil (5.4−4.5%), Antarctic krill oil (9−9.5%), and fungal oil (2%). Six HMFSs were prepared, among which HMFS V and VI were similar to human milk fat from Chinese in terms of fatty acid composition and triacylglycerol species. The sn-2 position of HMFS was occupied by palmitic acid (49.31 and 43.48% in HMFS V and VI, respectively). The major triacylglycerols were OPL, OPO, and LPL, accounting for 15. 90, 9.49, and 6.84 and 17.52, 8.44, and 8.55% in HMFS V and VI, respectively. This study is the first to prepare structured lipids intended for infant formula through fermentation, providing a novel strategy for the edible oil industry.

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Section: Results and Discussionsupporting

confidence: 92%

“…More specifically, R. opacus accumulates saturated fatty acids, especially C16:0, at the sn -2 position. ,, When a suitable carbon source is provided in the medium, HMFS is produced.…”

Section: Results and Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Facile and Green Production of Human Milk Fat Substitute through Rhodococcus opacus Fermentation

Zhang

Chu

Zong

et al. 2020

J. Agric. Food Chem.

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“…Specifically, if the chain length was less than 12, the carbon source entered the fatty acid elongation pathway and formed palmitic acid or oleic acid. When oleic acid, palmitic acid and linoleic acid were supplied as substrate fats, 1-oleoyl-2-palmityl-3-linoleoyl glycerol (OPL) was formed [152,153].…”

Section: Fermentation With Microorganismsmentioning

confidence: 99%

Preparation of Human Milk Fat Substitutes: A Review

Jiang

Zou

Chao

et al. 2022

Life

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Human milk is generally regarded as the best choice for infant feeding. Human milk fat (HMF) is one of the most complex natural lipids, with a unique fatty acid composition and distribution and complex lipid composition. Lipid intake in infants not only affects their energy intake but also affects their metabolic mode and overall development. Infant formula is the best substitute for human milk when breastfeeding is not possible. As the main energy source in infant formula, human milk fat substitutes (HMFSs) should have a composition similar to that of HMF in order to meet the nutritional needs of infant growth and development. At present, HMFS preparation mainly focuses on the simulation of fatty acid composition, the application of structured lipids and the addition of milk fat globule membrane (MFGM) supplements. This paper first reviews the composition and structure of HMF, and then the preparation development of structured lipids and MFGM supplements are summarized. Additionally, the evaluation and regulation of HMFSs in infant formula are also presented.

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“…Human milk fat is considered the best source of dietary fat for infants, providing 50% of the dietary energy demand for infants [ 103 ], in addition to being an important source of essential fatty acids, fat-soluble vitamins, and bioactive compounds [ 104 ]. Human milk fat TAG’s composition consists mainly of linoleic acid, palmitic acid, and oleic acid.…”

Section: Enzymatic Preparation and Application Of Slsmentioning

confidence: 99%

Future of Structured Lipids: Enzymatic Synthesis and Their New Applications in Food Systems

Zhou

Lee

Mao

et al. 2022

Foods

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Structured lipids (SLs) refer to a new type of functional lipid obtained by modifying natural triacylglycerol (TAG) through the restructuring of fatty acids, thereby altering the composition, structure, and distribution of fatty acids attached to the glycerol backbones. Due to the unique functional characteristics of SLs (easy to absorb, low in calories, reduced serum TAG, etc.), there is increasing interest in the research and application of SLs. SLs were initially prepared using chemical methods. With the wide application of enzymes in industries and the advantages of enzymatic synthesis (mild reaction conditions, high catalytic efficiency, environmental friendliness, etc.), synthesis of SLs using lipase has aroused great interest. This review summarizes the reaction system of SL production and introduces the enzymatic synthesis and application of some of the latest SLs discussed/developed in recent years, including medium- to long-chain triacylglycerol (MLCT), diacylglycerol (DAG), EPA- and DHA-enriched TAG, human milk fat substitutes, and esterified propoxylated glycerol (EPG). Lastly, several new ways of applying SLs (powdered oil, DAG plastic fat, inert gas spray oil, and emulsion) in the future food industry are also highlighted.

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Efficient Production of 1,3‐Dioleoyl‐2‐Palmitoylglycerol through Rhodococcus opacus Fermentation

Cited by 14 publications

References 31 publications

Facile and Green Production of Human Milk Fat Substitute through Rhodococcus opacus Fermentation

Facile and Green Production of Human Milk Fat Substitute through Rhodococcus opacus Fermentation

Preparation of Human Milk Fat Substitutes: A Review

Future of Structured Lipids: Enzymatic Synthesis and Their New Applications in Food Systems

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