Efficient production of lactic acid from sucrose and corncob hydrolysate by a newly isolated Rhizopus oryzae GY18

Abstract

The aim of this study is to investigate production of L-lactic acid from sucrose and corncob hydrolysate by the newly isolated R. oryzae GY18. R. oryzae GY18 was capable of utilizing sucrose as a sole source, producing 97.5 g l(-1) L-lactic acid from 120 g l(-1) sucrose. In addition, the strain was also efficiently able to utilize glucose and/or xylose to produce high yields of L-lactic acid. It was capable of producing up to 115 and 54.2 g l(-1) lactic acid with yields of up to 0.81 g g(-1) glucose and 0.90 g g(-1) xylose, respectively. Corncob hydrolysates obtained by dilute acid hydrolysis and enzymatic hydrolysis of the cellulose-enriched residue were used for lactic acid production by R. oryzae GY18. A yield of 355 g lactic acid per kg corncobs was obtained after 72 h incubation. Therefore, sucrose and corncobs could serve as potential sources of raw materials for efficient production of lactic acid by R. oryzae GY18.

L‐lactic acid production by Rhizopus oryzae MBG‐10 using starch‐rich waste loquat kernels as substrate

“…At the other CaCO 3 concentrations (0, 10, and 30 g/L), determined pHs were 4.4, 5.1, and 6.0, respectively. Optimal CaCO 3 concentration of 20 g/L was lower than those reported in the previous studies [25][26][27]. For example, Wu et al [27] reported that the maximum lactic acid production by R. oryzae was achieved when 50 g/L CaCO 3 were used as neutralizing agent.…”

Lactic Acid Production to Purification: A Review

“…Rhizopus species, such as R. oryzae and R. arrhizus, have amylolytic enzymatic activity, which enables them to convert starch directly to L(+)-lactic acid. Several studies have reported on the use of Rhizopus for lactic acid production (Yin et al 1998;Liu et al 2006;Yu et al 2007;Guo et al 2010;Wu et al 2011;Saito et al 2012). Fig.…”

Zygomycetes-based biorefinery: Present status and future prospects

“…However, other production routes, using starchy and lignocellulosic materials as more cost-effective feedstocks, have also been explored (Zhang et al, 2007). In addition to glucose, sucrose is being examined for L-lactic acid production; the yield of 0.81 g/g is however difficult to obtain on a regular basis (Guo et al, 2010). In a recent study concerning the use of starch-containing substrates, a yield of 0.72 g L-lactic acid per gram potato starch was attained, when using Rhizopus in pellet form (Yen & Lee, 2010).…”