Efficient production of (R,R)-2,3-butanediol from cellulosic hydrolysate using Paenibacillus polymyxa ICGEB2008

Adlakha, Nidhi; Yazdani, Syed Shams

doi:10.1007/s10295-014-1542-0

Cited by 33 publications

(19 citation statements)

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“…Our experiments confirmed this for ICGEB2008 showing a maximal yield of 0.32 g 2,3-butanediol per g of glucose (~0.49 mM 2,3-butanediol/mM glucose) (Fig. 1 ) [ 14 ]. In addition, we also observed ethanol secreted with a yield of 0.18 g per g glucose (~0.7 mM ethanol/mM glucose), besides small amounts of acetic acid, acetone and lactic acid.…”

Section: Resultssupporting

confidence: 80%

“…This strain was isolated from the gut of a cotton bollworm and was shown to produce a number of cellulolytic enzymes [ 12 , 13 ]. The strain also showed the ability of producing high yields of 2,3-butanediol [ 14 ]. In combination, these metabolic capabilities make this strain an interesting candidate for biotechnological purposes, which include the conversion of biomass to combustible fuels or valuable chemicals.…”

Section: Introductionmentioning

confidence: 99%

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Insight into metabolic pathways of the potential biofuel producer, Paenibacillus polymyxa ICGEB2008

Adlakha

Pfau

Ebenhöh

et al. 2015

Biotechnol Biofuels

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BackgroundPaenibacillus polymyxa is a facultative anaerobe known for production of hydrolytic enzymes and various important biofuel molecules. Despite its wide industrial use and the availability of its genome sequence, very little is known about metabolic pathways operative in the Paenibacillus system. Here, we report metabolic insights of an insect gut symbiont, Paenibacillus polymyxa ICGEB2008, and reveal pathways playing an important role in the production of 2,3-butanediol and ethanol.ResultWe developed a metabolic network model of P. polymyxa ICGEB2008 with 133 metabolites and 158 reactions. Flux balance analysis was employed to investigate the importance of redox balance in ICGEB2008. This led to the detection of the Bifid shunt, a pathway previously not described in Paenibacillus, which can uncouple the production of ATP from the generation of reducing equivalents. Using a combined experimental and modeling approach, we further studied pathways involved in 2,3-butanediol and ethanol production and also demonstrated the production of hydrogen by the organism. We could further show that the nitrogen source is critical for metabolite production by Paenibacillus, and correctly quantify the influence on the by-product metabolite profile of ICGEB2008. Both simulations and experiments showed that metabolic flux is diverted from ethanol to acetate production when an oxidized nitrogen source is utilized.ConclusionWe have created a predictive model of the central carbon metabolism of P. polymyxa ICGEB2008 and could show the presence of the Bifid shunt and explain its role in ICGEB2008. An in-depth study has been performed to understand the metabolic pathways involved in ethanol, 2,3-butanediol and hydrogen production, which can be utilized as a basis for further metabolic engineering efforts to improve the efficiency of biofuel production by this P. polymyxa strain.Electronic supplementary materialThe online version of this article (doi:10.1186/s13068-015-0338-4) contains supplementary material, which is available to authorized users.

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Section: Resultssupporting

confidence: 80%

Section: Introductionmentioning

confidence: 99%

Insight into metabolic pathways of the potential biofuel producer, Paenibacillus polymyxa ICGEB2008

Adlakha

Pfau

Ebenhöh

et al. 2015

Biotechnol Biofuels

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“…However, a majority of native producing strains are known to be pathogenic, hence cannot be used for industrial production of 2,3‐BD . The native pathogenic high producers of 2,3‐BD include Klebsiella pneumonia, Serratia marcescens and Klebsiella oxytoca while Bacillus polymyxa, B. subtilis , Bacillus amyloliquefaciens and Bacillus licheniformis strains which have been regarded as non‐pathogenic bacteria can be used for its industrial‐scale production. In recent years, non‐native producers of 2,3‐BD like Escherichia coli have been engineered and used to produce it in high concentration .…”

Section: Introductionmentioning

confidence: 99%

Optimized whole cell biocatalyst from acetoin to 2,3‐butanediol through coexpression of acetoin reductase with NADH regeneration systems in engineered Bacillus subtilis

Samuel

Teng

Zhang

et al. 2017

J of Chemical Tech & Biotech

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BACKGROUND: 2,3-Butanediol (2,3-BD) has a wide range of applications in chiral molecular synthesis, biofuel additives, and in food flavor additive manufacturing. Fermentation is a favorable method for 2,3-BD production. However, it requires much time and produces several NADH related byproducts which compete with 2,3-BD production. Bacillus subtilis has an excellent ability for 2,3-BD production by biocatalysis. However, its production is limited by low intracellular NADH and the reversible property of acetoin reductase (AR/2,3-BDH). The whole cell biocatalyst process with two different NADH regeneration systems was designed for efficient production of 2,3-BD in B. subtilis 168. RESULTS: Formate dehydrogenase and glucose dehydrogenase for NADH regeneration were successfully co-expressed with acetoin reductase in B. subtilis 168. After optimization of biocatalyst bioconversion conditions, B. subtilis 168/pMA5-bdhA-HpaII-fdh yielded 74.5 g L − 1 of 2, 3-BD with 9.3 g L −1 h −1 productivity by fed batch and 115.4 g of 2,3-BD was achieved using same batch bacterium by three repeated batch bioconversions. On the other hand, 63.7 g L −1 of 2, 3-BD was produced with 7.92 g L −1 h −1 productivity by B. subtilis 168/pMA5-bdhA-HpaII-gdh. To our knowledge, the volume productivity obtained here is the highest ever reported for biocatalysis. CONCLUSION: A higher productivity of 2,3-BD from acetoin was achieved by whole cell biocatalysis with NADH regeneration systems in B. subtilis 168. This approach can be applied for NADH related bio-based chemicals production to improve titer, yield and productivity.

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“…Microbial production of 2,3-butanediol (2,3-BD) is one such example. As an important platform intermediate, 2,3-BD could be used to produce numerous other chemical compounds such as methylethyl ketone, butadiene, and butanone (Celinska and Grajek 2009;Ji et al 2011;Syu 2011;Adlakha and Yazdani 2015). It can also be used as a fuel additive due to its combustion heat energy of 27.2 kJ/g Yang et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Utilization of Sweet Sorghum Juice for Efficient 2,3-Butanediol Production by Serratia marcescens H30

Yuan¹,

He²,

Guo³

et al. 2017

BioResources

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Sweet sorghum juice (SSJ) is considered a good carbon source for biorefinery due to its low price and high fermentable-sugar content. In this study, 2,3-butanediol (2,3-BD) production from SSJ by Serratia marcescens H30 was investigated. First, the medium compositions including the contents of SSJ, nitrogen source, and mineral salts were optimized in conical flasks using a single factor and orthogonal design method. Under the optimal conditions, the 2,3-BD concentration reached up to 33.40 g/L. Then the optimized medium was used to perform fermentative experiments in a 5-L bioreactor. In batch experiments, the effects of various agitation speeds on 2,3-BD production were compared. Based on batch process results, an efficient two-stage fermentative control strategy was developed, where the agitation speed was maintained at 300 rpm in the first 12 h and subsequently switched to 200 rpm. About 43.32 g/L 2,3-BD was obtained by using this strategy. Finally, fed-batch fermentation was conducted through feeding the concentrated SSJ and a maximum 2,3-BD concentration of 109.44 g/L with the productivity of 1.40 g/L·h; a yield of 83.02% was achieved. The results showed that SSJ could be used as an economical substrate for efficient 2,3-BD production by S. marcescens H30.

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Efficient production of (R,R)-2,3-butanediol from cellulosic hydrolysate using Paenibacillus polymyxa ICGEB2008

Cited by 33 publications

References 28 publications

Insight into metabolic pathways of the potential biofuel producer, Paenibacillus polymyxa ICGEB2008

Insight into metabolic pathways of the potential biofuel producer, Paenibacillus polymyxa ICGEB2008

Optimized whole cell biocatalyst from acetoin to 2,3‐butanediol through coexpression of acetoin reductase with NADH regeneration systems in engineered Bacillus subtilis

Utilization of Sweet Sorghum Juice for Efficient 2,3-Butanediol Production by Serratia marcescens H30

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