Efficient Protocol for Introducing Regeneration of Adventitious Buds from Hypocotyl Explants in Jatropha curcas L.

Abstract

A new strategy of high concentration short duration treatment of Thidiazuron (TDZ) for enhancing adventitious buds formation from hypocotyl explants were attempted in Jatropha curcas. The best shoot buds induction (81.91%) and number of buds per explant (10.16) were obtained from hypocotyl explants treated with 20 mg/L TDZ solution for 20 min, followed by 30 days of culture on hormone-free MS medium. The elongated shoots initiated roots to become intact plantlets in rooting medium containing indole-3-butyric acid (IBA) and L-glutamine (Gln), and the results showed that the initiation and growth of roots were effectively stimulated with supplementing 16 mg/L Gln into the rooting medium, and having the best rooting rate (51.39%). After acclimatization, an intact plantlet could usually be gained at 60 days of culture by using the culture protocol described in this study.