Efficient separation determination of protopanaxatriol ginsenosides Rg1, Re, Rf, Rh1, Rg2 by HPLC

Zhang, Lina; Wang, Shaoyan; Qu, Boquan; Chi, Haijun; Quan, Yanling; Wu, Xiuhong

doi:10.1016/j.jpba.2019.03.025

Cited by 14 publications

(6 citation statements)

References 29 publications

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“…Multicomponent quantitation by various chromatography hyphenation techniques, such as LC-UV, LC-ELSD, LC-CAD, LC-MS, and GC-MS, or the direct-profiling FT-NIR, is widely accepted for evaluating the quality of ginseng with different specifications and growing regions, or to differentiate various ginseng types derived from different plant parts. A summary of the available literature in this field is provided in Table S5 † 25,28,29,39,40,214,217,223,224,239,260,264,266,270,273–293.…”

Section: Quality Controlmentioning

confidence: 99%

“…From these quantitative assay experiments, the content variation ranges of the common ginsenosides, including six PPD-type (Rb1, Rb2, Rb3, Rc, Rd, and Rg3) and six PPT-type (Re, Rf, Rh1, Rg1, Rg2, and noto-R1), among PG (root, leaf, and berry)/RG/PQ/PN/PS ( P. sokpayensis )/PB ( P. bipinnatifidus ), are given in Table 5. 28,39,40,217,223,264,275,280,282,284,293 The contents of ginsenosides among different ginseng species showed significant differences. First, saponins in P. notoginseng were the richest, especially Rb1 as high as 40.7 mg g −1 .…”

Section: Quality Controlmentioning

confidence: 99%

See 1 more Smart Citation

Advances and challenges in ginseng research from 2011 to 2020: the phytochemistry, quality control, metabolism, and biosynthesis

Liu

Zuo

et al. 2022

Nat. Prod. Rep.

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Section: Quality Controlmentioning

confidence: 99%

Section: Quality Controlmentioning

confidence: 99%

Advances and challenges in ginseng research from 2011 to 2020: the phytochemistry, quality control, metabolism, and biosynthesis

Liu

Zuo

et al. 2022

Nat. Prod. Rep.

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“…The final product of the metabolism of protopanaxatriol ginsenosides (Rh1, Rg1, Re, and others) by intestinal microorganisms is 20(S)-PPT. The structure of 20(S)-PPT is characterized by a hydroxyl group at C-6 position, which categorizes it as a damarane saponin 15 . On the one hand, many studies have shown that panax notoginseng saponins can improve AS, and the mechanism of action involves: (1) Enhancement transcriptional activation of the LXRα gene promoter, subsequent upregulation of ABCA1 and ABCG1 and inhibition of NF-κB DNA binding activity.…”

Section: Introductionmentioning

confidence: 99%

20(S)-Protopanaxatriol Improves Atherosclerosis by Inhibiting Low-Density Lipoprotein Receptor Degradation in ApoE KO Mice

Zhang¹,

Luo²,

Litian³

et al. 2023

Preprint

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Atherosclerosis (AS) is a chronic progressive disease caused by various factors, and causes various cerebrovascular and cardiovascular diseases (CVDs). Reducing the plasma levels of low-density lipoprotein-cholesterol (LDL-C) is the primary goal in preventing and treating AS. Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) plays a crucial role in regulating LDL-C metabolism. Panax notoginseng has potent lipid-reducing effects and protects against CVDs, and its saponins induce vascular dilatation, inhibit thrombus formation, and are used in treating CVDs. However, the anti-AS effect of the secondary metabolite, 20(S) -protopanaxatriol (20(S)-PPT), remains unclear. In this study, the anti-AS effect and molecular mechanism of 20(S)-PPT were investigated in vivo and in vitro by western blotting, real time-polymerase chain reaction (RT-PCR), Enzyme-linked Immunosorbent Assay (ELISA), immunofluorescence staining, and other assays. The in vitro experiments revealed that 20(S)-PPT reduced the levels of PCSK9 in the supernatant of HepG2 cells, upregulated low density lipoprotein receptor (LDLR) protein levels, promoted LDL uptake by HepG2 cells, and reduced PCSK9 mRNA transcription by upregulating the levels of FoxO3 protein and mRNA and decreasing the levels of HNF1α protein and mRNA. The in vivo experiments revealed that 20(S)-PPT upregulated aortic αSMA expression, increased the stability of atherosclerotic plaques, and reduced aortic plaque formation induced by a high-cholesterol fed in ApoE-/- mice (HCF group). Additionally, 20(S)-PPT reduced the aortic expression of CD68, reduced inflammation in the aortic root, and alleviated the hepatic lesions in the HCF group. The study revealed that 20(S)-PPT inhibited LDLR degradation via PCSK9 to alleviate AS.

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“…Recent studies have characterized ginsenosides through HPLC and HPLC-MS [ 9 , [15] , [16] , [17] ]. Because of the development of HPLC-MS with high sensitivity and resolution, precision of quantitation of TCM has been significantly improved.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of storage period of fresh ginseng for quality improvement of dried and red processed varieties

Zhang

Huang

Guo

et al. 2022

Journal of Ginseng Research

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Efficient separation determination of protopanaxatriol ginsenosides Rg1, Re, Rf, Rh1, Rg2 by HPLC

Cited by 14 publications

References 29 publications

Advances and challenges in ginseng research from 2011 to 2020: the phytochemistry, quality control, metabolism, and biosynthesis

Advances and challenges in ginseng research from 2011 to 2020: the phytochemistry, quality control, metabolism, and biosynthesis

20(S)-Protopanaxatriol Improves Atherosclerosis by Inhibiting Low-Density Lipoprotein Receptor Degradation in ApoE KO Mice

Evaluation of storage period of fresh ginseng for quality improvement of dried and red processed varieties

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