Efficient Soluble Expression and Purification of Recombinant Human Acidic Fibroblast Growth Factor from Escherichia coli via Fusion with a Novel Collagen-like Protein Scl2

Recombinant and genetic code expanded collagen-like protein as a tailorable biomaterial

“…The fusion protein expression of recombinant acidic or basic fibroblast growth factor together with the Scl2 protein achieved the soluble expression of recombinant fibroblast growth factor, in contrast to expression in insoluble inclusion bodies without Scl2 fusion. 100,101 In addition, the blank slate Scl2 protein could be tailored to identify new cell binding sites present in animal collagen and its biological responses in stimulating cellular functions by introducing cell binding sites. 102 The different recombinant expression systems for collagenous protein, the biomaterial design and in vitro evaluations for significant cell interactions, adhesion and proliferation are tabulated in Table 3.…”