Efficient utilization of carbon to produce aromatic valencene in <i>Saccharomyces cerevisiae</i> using mannitol as the substrate

Zhu, Chaoyi; You, Xiao; Wu, Tao; Li, Wen; Chen, Hefeng; Cha, Yaping; Zhuo, Min; Chen, Bin; Li, Shuang

doi:10.1039/d2gc00867j

Cited by 15 publications

(11 citation statements)

References 68 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…13 Although the theoretical yield of LA is 1.0 g/g glucose, in the context of the metabolic background in S. cerevisiae, the energy and redox equivalent requirements need to be considered. 36 In reality, the yield of LA using S. cerevisiae as a host is much lower than the maximum theoretical yield. Several studies have been reported to produce LA under low-pH conditions (Table 1); however, a low concentration of neutralizer is still needed during LA production.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

See 1 more Smart Citation

l-Lactic Acid Production via Sustainable Neutralizer-Free Route by Engineering Acid-Tolerant Yeast Pichia kudriavzevii

Zhang

Li²,

Liu³

et al. 2023

J. Agric. Food Chem.

View full text Add to dashboard Cite

l-Lactic acid (l-LA) is a platform chemical obtained via microbial fermentation at a near-neutral pH value. Large amounts of neutralizers are required during this process, which increases the production costs in downstream processing as well as environmental burden. To address this challenge, an acid-tolerant yeast Pichia kudriavzevii E1 was isolated and metabolically engineered to produce l-LA without neutralizers. The genome of strain E1 was sequenced and a CRISPR-Cas9 system was developed in this newly isolated strain. Subsequently, the gene encoding pyruvate decarboxylase (pdc) was knocked out to subdue ethanol formation. Furthermore, the l-lactate dehydrogenase gene from Weizmannia coagulans 2-6 and the codon-optimized L-ldhA gene from Bos taurus were introduced into P. kudriavzevii E1 chromosome to redirect the ethanol fermentation pathway to l-LA production. Deletion of the dld(chr3) gene further increased the optical purity of l-LA. After optimizing fermentation conditions, the maximum titer of l-LA in the 5 L fermenter reached 74.57 g/L without any neutralizers, with an optical purity of 100% and a maximum yield of 0.93 g/g glucose. This is the first report of optically pure l-LA production without neutralizers and the engineered acid-tolerant yeast paves the way for the sustainable production of l-LA via a green route.

show abstract

Section: ■ Results and Discussionmentioning

confidence: 99%

“…Although the theoretical yield of LA is 1.0 g/g glucose, in the context of the metabolic background in S. cerevisiae, the energy and redox equivalent requirements need to be considered . In reality, the yield of LA using S.…”

Section: Resultsmentioning

confidence: 99%

l-Lactic Acid Production via Sustainable Neutralizer-Free Route by Engineering Acid-Tolerant Yeast Pichia kudriavzevii

Zhang

Li²,

Liu³

et al. 2023

J. Agric. Food Chem.

View full text Add to dashboard Cite

show abstract

“…Mannitol, a major component of brown seaweed, is considered as a sustainable and efficient carbon source due to its wide availability and high reducing power yielded per carbon during the process of mannitol metabolism. 10 In addition, mannitol is a nonfermentable carbon source for S. cerevisiae, meaning it works in a respiration-dependent manner without causing the Crabtree effect and ethanol overproduction (Figure 1). Considering the effect of climatic factors on the sugar content in seaweed, 22 seaweeds from two distinct regions of China, namely, Shandong Province and Fujian Province, were analyzed.…”

Section: Resultsmentioning

confidence: 99%

“…Significant progress has been made in developing microbial cell-based platforms, such as S. cerevisiae, for valencene production. , While glucose has traditionally been utilized as the carbon source for valencene production, the pioneering work of Zhu et al has shown that high yields of valencene can be achieved using mannitol instead, which presents new opportunities for utilizing seaweed biomass in valencene production.…”

Section: Introductionmentioning

confidence: 99%

Green Production of a High-value Mosquito Insecticide of Nootkatone from Seaweed Hydrolysates

Ma,

Wu,

Liu

et al. 2023

J. Agric. Food Chem.

Self Cite

View full text Add to dashboard Cite

“…The final engineered strain yielded 1.2 g L −1 of valencene. A similar study was also reported by Zhu et al, 81 yielding 5.61 g L −1 valencene. Surprisingly, Ye et al 82 also engineered S. cerevisiae to produce a high level of (+)-valencene.…”

Section: Chemical Synthesismentioning

confidence: 99%

Production, Function, and Applications of the Sesquiterpenes Valencene and Nootkatone: a Comprehensive Review

Zhang

Chen

et al. 2022

J. Agric. Food Chem.

View full text Add to dashboard Cite

Valencene and nootkatone, two sesquiterpenes, extracted from natural sources, have great market potential with diverse applications. This paper aims to comprehensively review the recent advances in valencene and nootkatone, including source, production, physicochemical and biological properties, safety and pharmacokinetics evaluation, potential uses, and their industrial applications as well as future research directions. Microbial biosynthesis offers a promising alternative approach for sustainable production of valencene and nootkatone. Both compounds exert various beneficial activities, including antimicrobial, insecticidal, antioxidant, anti-inflammatory, anticancer, cardioprotective, neuroprotective, hepatoprotective, and nephroprotective and other activities. However, most of the studies are performed in animals and in vitro, making it difficult to give a conclusive description about their health benefits and extend their application. Hence, more attention should be paid to in vivo and long-term clinical studies in the future. Moreover, valencene and nootkatone are considered safe for consumption and show great promise in the applications of food, cosmetic, pharmaceutical, chemical, and agricultural industries.

show abstract

Efficient utilization of carbon to produce aromatic valencene in Saccharomyces cerevisiae using mannitol as the substrate

Abstract: As a promising brown macroalgae carbohydrate, mannitol has been an alternative renewable feedstock for biofuel and biochemical production, avoiding competition with food production for natural resources. In this study, we...

Cited by 15 publications

References 68 publications

l-Lactic Acid Production via Sustainable Neutralizer-Free Route by Engineering Acid-Tolerant Yeast Pichia kudriavzevii

l-Lactic Acid Production via Sustainable Neutralizer-Free Route by Engineering Acid-Tolerant Yeast Pichia kudriavzevii

Green Production of a High-value Mosquito Insecticide of Nootkatone from Seaweed Hydrolysates

Production, Function, and Applications of the Sesquiterpenes Valencene and Nootkatone: a Comprehensive Review

Contact Info

Product

Resources

About