Efflux Pump Inhibitor Phenylalanine-Arginine Β-Naphthylamide Effect on the Minimum Inhibitory Concentration of Imipenem in Acinetobacter baumannii Strains Isolated From Hospitalized Patients in Shahid Motahari Burn Hospital, Tehran, Iran

Goli, Hamid Reza; Hashemi, Ali; Hakemi-Vala, Mojdeh; Goudarzi, Hossein; Hallajzadeh, Masoumeh

doi:10.5812/jjm.19048

Cited by 32 publications

(14 citation statements)

References 18 publications

(25 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It was reported that the imipenem susceptibility of most A. baumannii isolated from burns was increased in the presence of the PAβN by 4 to 64 folds (14). Our results indicated the role of efflux pump mechanism in imipenem and meropenem resistance in A. baumannii isolates.…”

Section: The Role Of Adeabc Efflux Pump Genes In Multidrug and Carbapsupporting

confidence: 54%

“…In another conducted study from Iran on 60 A. baumannii isolates from burn units, the results revealed the detection of adeA and adeB genes in all isolates (100%) while AdeC was present in 51 isolates (85%) (14). In another study by Hou et al (15) it was found that adeB, adeR, adeS, adeJ and abeM were found in the majority of imipenem resistant A. baumannii (> 80% …”

Section: Detection and Distribution Of Adeabc Efflux Pump Genesmentioning

confidence: 91%

See 1 more Smart Citation

AdeABC Efflux Pump Genes in Multidrug ResistantAcinetobacter baumanniiIsolates

Jassim¹,

Ghaima²,

Saadedin³

2016

Avicenna J Clin Microbiol Infect

View full text Add to dashboard Cite

Objectives: Acinetobacter baumannii is an opportunistic nosocomial pathogen and a cause of severe infections in hospitalized patients, owing to its ability to acquire drug resistance by the efflux pump mechanism. The current study investigated the detection and prevalence of efflux pump genes (AdeABC) in multidrug resistant A. baumannii isolates and the role of these genes in multidrug and carbapenems resistance. Methods: This study was conducted on 84 multidrug resistant and 13 carbapenem-susceptible A. baumannii isolates obtained from burn and wound infections in Baghdad hospitals, Iraq. The AdeABC genes were detected by polymerase chain reaction (PCR) assay. Phe-arg-beta-naphthylamide (PAβN)-based method was used for determination of efflux pump activity. Results: This study showed high prevalence of efflux pump genes in our local isolates. The AdeB gene was present in all multidrug resistant isolates (100%) while AdeRS gene was found in 95.2%, AdeC gene in 83.3% and AdeA gene in 77.4%. By comparing the prevalence of these gene in carbapenem-susceptible isolates, it was demonstrated that the gene AdeB was absent in all susceptible isolates, also the regulatory gene AdeRS was not found in most of these isolates, while the other genes (AdeA and AdeC) were detected in most carbapenem-susceptible isolates. Susceptibility of isolates to Amikacin, Gentamicin, Ciprofloxacin, Levofloxacin, Tetracycline and Tigecycline was highly increased in the presence of efflux pump inhibitor, so that, PAβN reduced the minimum inhibitory concentrations (MICs) by 4 to 32 folds. Also, MICs of carbapenems were reduced in the presence of the inhibitor by two to eight folds, while the MICs of colistin were not affected. Conclusions: AdeABC efflux system plays a vital role in multidrug resistance in clinical A. baumannii isolates. It was noted that the most important gene responsible for multidrug resistance within this system was the AdeB gene especially in carbapenems resistance.

show abstract

Section: The Role Of Adeabc Efflux Pump Genes In Multidrug and Carbapsupporting

confidence: 54%

Section: Detection and Distribution Of Adeabc Efflux Pump Genesmentioning

confidence: 91%

AdeABC Efflux Pump Genes in Multidrug ResistantAcinetobacter baumanniiIsolates

Jassim¹,

Ghaima²,

Saadedin³

2016

Avicenna J Clin Microbiol Infect

View full text Add to dashboard Cite

show abstract

“…The nosocomial infections, caused by multidrug-resistant (MDR) bacteria, are one of the most important causes of mortality throughout the world (3). Therefore, multidrug-resistant Gram-negative rods will be a serious problem if no adequate action is taken (4,5). Unfortunately, the issue of antibiotic resistant bacteria in Iran is increasing, following the rising trend of its global counterpart (6).…”

Section: Contextmentioning

confidence: 99%

“…For every carbapenemase activity, the sensitivity and specificity of it is 100% and 98.5%, respectively. In addition, this method has the accuracy to differentiate carbapenemase producers from non-carbapenemase producers, among carbapenem-nonsusceptible isolates [extended spectrum beta-lactamases (ESBLs), cephalosporinase producers and fault in outer 4 Arch Clin Infect Dis. 2016; 11(2):e32816.…”

Section: Ultraviolet Spectrophotometermentioning

confidence: 99%

Laboratory Detection of Carbapenemases in Gram-Negative Bacteria

Goudarzi

Taherpour

Fallah

et al. 2016

Arch Clin Infect Dis

Self Cite

View full text Add to dashboard Cite

The nosocomial infections, caused by multi-drug resistant bacteria, are the most important cause of mortality throughout the world. One important mechanism against beta-lactam antibiotics is the production of beta-lactamases. Enterobacteriaceae and nonfermentative bacteria, like Pseudomonas aeruginosa and Acinetobacter baumannnii may produce these enzymes. Currently, there is no vaccine to prevent the infections caused by β-lactamase-producing bacteria. Consequently, it is necessary to identify β-lactamaseproducing bacteria by phenotypic and molecular methods.

show abstract

“…Fallah et al reported that the resistance to colistin was found in two A. baumannii strains (1.8%) (16). Gholami et al reported no resistance to colistin among the A. baumannii strains (19). Therefore, colistin could be useful in curing infections that A. baumannii cause, mainly in burn patients.…”

Section: Discussionmentioning

confidence: 99%

Efflux Pump Inhibitor Carbonyl Cyanide 3-Chlorophenylhydrazone (CCCP) Effect on the Minimum Inhibitory Concentration of Ciprofloxacin in Acinetobacter baumannii Strains

Moazzen

Eslami

Hashemi

et al. 2018

Arch Clin Infect Dis

Self Cite

View full text Add to dashboard Cite

Background: One of the most important pathogens with increasing mortality rate in hospitalized patients is Acinetobacter baumannii. Objectives: Therefore, this study aimed at detecting multidrug-resistance and the role of efflux pumps in Ciprofloxacin resistance of A. baumannii strains isolated from burn patients. Methods: Eighty A. baumannii strains collected from 240 wound samples were isolated from burn patients hospitalized in the burn unit of Shahid Motahari hospital. Susceptibility to antibiotics was tested by disc diffusion and microdilution methods. Activity of the efflux pump system was assessed using the efflux pump inhibitor, Carbonyl Cyanide 3-Chlorophenylhydrazone (CCCP). Polymerase Chain Reaction (PCR) and sequencing were done for detecting the AdeABC genes. Results: Overall, 100% of A. baumannii isolates were resistant to ceftriaxone, ceftazidime, cefepime, cefotaxime, meropenem, ciprofloxacin, co-trimoxazole, piperacilin, piperacillin/tazobactam and imipenem; 72 (90%) to amikacin, 72 (90%) to gentamicin, and 0 (0.0%) to colistin The high increase of the susceptibility to ciprofloxacine in the strains in the presence of the efflux pump inhibitor was remarkable. In 25% of isolates, CCCP decreased the minimum inhibitory concentrations (MIC) by 4 to 64 folds. In 80 (100%) of the isolates, adeb, adeA, and adeC genes were detected. Conclusions:The results indicated the role of efflux pump in antibiotic resistance in A. baumannii isolates. Furthermore, A. baumannii strains could resist antibiotics by the efflux mechanism.

show abstract

Efflux Pump Inhibitor Phenylalanine-Arginine Β-Naphthylamide Effect on the Minimum Inhibitory Concentration of Imipenem in Acinetobacter baumannii Strains Isolated From Hospitalized Patients in Shahid Motahari Burn Hospital, Tehran, Iran

Cited by 32 publications

References 18 publications

AdeABC Efflux Pump Genes in Multidrug ResistantAcinetobacter baumanniiIsolates

AdeABC Efflux Pump Genes in Multidrug ResistantAcinetobacter baumanniiIsolates

Laboratory Detection of Carbapenemases in Gram-Negative Bacteria

Efflux Pump Inhibitor Carbonyl Cyanide 3-Chlorophenylhydrazone (CCCP) Effect on the Minimum Inhibitory Concentration of Ciprofloxacin in Acinetobacter baumannii Strains

Contact Info

Product

Resources

About