EGF-Loaded Nanofibers for Skin Tissue Engineering

Norouzi, Mohammad; Soleimani, Masoud

doi:10.1016/b978-0-12-802926-8.00028-8

Cited by 5 publications

(2 citation statements)

References 45 publications

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“…However, the inert surface of biomaterials has limited control over the binding of bioactive molecules. , Alternatively, a nanofiber surface modified with anionic or cationic molecules has been exploited for the affinity-based immobilization of growth factors, peptides, and nucleic acids. For example, several growth factors such as VEGF, fibroblast growth factor (FGF), EGF, and transforming growth factor β (TGF-β) exhibited strong binding affinity toward heparin-modified surfaces. ,− The affinity-based noncovalent binding of growth factors on nanofibers is a promising strategy for the prolonged retention of growth factors and the preservation of their bioactivities. Heparin could be chemically conjugated onto nanofibers containing amine groups through EDC/NHC chemistry ,, or could be conjugated with polymers that were then blended with a base spinning polymer.…”

Section: Fabrication Of Bioactive Electrospun Fibersmentioning

confidence: 99%

Bioactive Electrospun Fibers: Fabrication Strategies and a Critical Review of Surface-Sensitive Characterization and Quantification

et al. 2021

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Fabricating a porous scaffold with high surface area has been a major strategy in the tissue engineering field. Among the many fabrication methods, electrospinning has become one of the cornerstone techniques due to its enabling the fabrication of highly porous fibrous scaffolds that are of natural or synthetic origin. Apart from the basic requirements of mechanical stability and biocompatibility, scaffolds are further expected to embody functional cues that drive cellular functions such as adhesion, spreading, proliferation, migration, and differentiation. There are abundant distinct approaches to introducing bioactive molecules to have a control over cellular functions. However, the lack of a thorough understanding of cell behavior with respect to the availability and spatial distribution of the bioactive molecules in 3D fibrous scaffolds is yet to be addressed. The rational selection of proper sets of characterization techniques would essentially impact the interpretation of the cell–scaffold interactions. In this timely Review, we summarize the most popular methods to introduce functional compounds to electrospun fibers. Thereafter, the strength and limitations of the conventional characterization methods are highlighted. Finally, the potential and applicability of emerging characterization techniques such as high-resolution/correlative microscopy approaches are further discussed.

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Section: Fabrication Of Bioactive Electrospun Fibersmentioning

confidence: 99%

Bioactive Electrospun Fibers: Fabrication Strategies and a Critical Review of Surface-Sensitive Characterization and Quantification

et al. 2021

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“…Exogenous EGF supply might cause a more rapid re‐epithelialization, reduce infection risk, and enhance the speed and quality of wound healing 15,16 . Therefore, encapsulation of EGF in membranes with nanofibrous structures has the potential to help conquer some limitations, since these membranes are capable of protecting EGF from the environment and enhancing the stability of it 17,18 . According to several reports, in case of using growth factors, excluding scaffolds, numerous limitations, like the need for continuous growth factor supplements, difficult control of growth factor release, and low wound healing rates may arise 19,20 …”

Section: Introductionmentioning

confidence: 99%

Effects of bilayer nanofibrillar scaffolds containing epidermal growth factor on full‐thickness wound healing

Golchin

Nourani

2020

Polymers for Advanced Techs

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Wound healing refers to a complex, dynamic process in the body, especially the skin, which influences the quality of life of millions of people each year. Electrospinning is an efficient and economic technique for the production of micro-to nano-fibrillar constructs as drug delivery systems with biomedical applications. In this study, we designed an electrospun bilayer fibrillar scaffold immobilized with epidermal growth factor (EGF), using polycaprolactone (PCL) as the upper layer and chitosan (CS)/polyvinyl alcohol (PVA) as the lower layer of wound dressing. The scanning electron microscopy (SEM) was utilized to characterize the scaffolds. The water uptake, release potency, and toxicity of scaffolds were evaluated, and also then DAPI staining and MTT assay after seeding adipose tissue-derived mesenchymal stem cells. Finally, the effects of the scaffolds on the wound healing process were investigated in a mouse model of full-thickness skin injury. The results of SEM demonstrated continuous, smooth, bead-free, and randomly aligned fibers. The average diameter of CS/PVA nanofibers was 238.36 ± 36.99, and the mean diameter of PCL nanofibers was 1271.79 ± 428.49 nm. The scaffolds were capable of cell seeding and supported their growth and proliferation. Furthermore, in the comparison between designed composite as dressing patch and control group (Vaseline), our composite demonstrated significant differences, so that EGF-immobilized patch efficiently accelerated wound closure and improved histological healing in an in vivo full-thickness wound healing mouse model. The present results suggest the potential of the designed electrospun bilayer fibrillar scaffold containing EGF for the treatment of fullthickness wounds and skin regeneration. K E Y W O R D S chitosan/polyvinyl alcohol/polycaprolactone, epidermal growth factor (EGF), mesenchymal stem cells, skin regeneration, wound healing 1 | INTRODUCTION Skin is one of the vital body tissues, which is composed of dermis, epidermis, and hypoderm (subcutaneous fat) layers and covers the external body surface. It constitutes almost 8% of the total mass of the body and has several crucial functions. 1 It provides an effective barrier against adverse external conditions and has properties, which can prevent microbial, mechanical, chemical, osmotic, thermal, and photo damage. Skin wounds, which can be caused by burns, venous ulcers, diabetic ulcers, and acute injury, are categorized into several types. Generally, wound healing is a dynamic and complex process, which is able to restore the skin's routine functionality and anatomical

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Techniques to Fabricate Electrospun Nanofibers for Controlled Release of Drugs and Biomolecules

Rajput

Tyeb

Chatterjee

2022

Advances in Polymer Science

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EGF-Loaded Nanofibers for Skin Tissue Engineering

Cited by 5 publications

References 45 publications

Bioactive Electrospun Fibers: Fabrication Strategies and a Critical Review of Surface-Sensitive Characterization and Quantification

Bioactive Electrospun Fibers: Fabrication Strategies and a Critical Review of Surface-Sensitive Characterization and Quantification

Effects of bilayer nanofibrillar scaffolds containing epidermal growth factor on full‐thickness wound healing

Techniques to Fabricate Electrospun Nanofibers for Controlled Release of Drugs and Biomolecules

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