eHealth: Disease activity measures are related to the faecal gut microbiota in adult patients with ulcerative colitis

Abstract

After the first PCR (95°C for 3 min, 30 cycles of 95°C for 30 sec, 55°C for 30 sec, 72°C for 30 sec, and final extension at 72°C for 5 min), amplicons were purified with AMPure XP beads in a x0.8 sample-to-beads ratio. A second round of PCR (95°C for 3 min, 10 cycles of 95°C for 30 sec, 55°C for 30 sec, 72°C for 30 sec, and final extension at 72°C for 5 min) was carried out to add Illumina indexing primers, and libraries were purified with AMPure XP beads in a x1 sample-to-beads ratio. Finally, libraries were normalised, pooled, and sequenced on an Illumina MiSeq with a 600 PE kit by the addition of 10% PhiX to the final pool. Forward Primer: 5'TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG Reverse Primer: 5'GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC Sequences were classified using BION-meta, previously described in Ring et al. (Ring HC, Thorsen J, Saunte DM, et al. The follicular skin microbiome in patients with hidradenitis suppurativa and healthy controls. JAMA Dermatology.

Rosa laevigata Polysaccharides Ameliorate Dextran Sulfate Sodium‐Induced Ulcerative Colitis of Beagles through Regulating Gut Microbiota

“…isolated a polysaccharide from Ficus carica , which protected the goblet cells, elevated the expression of tight junction protein, suppressed the formation of proinflammation cytokines, and reformed the gut microbiome by enhancing the abundance of S24‐7 , Bacteroides , and Coprococus , and suppressing the abundance of Escherichia and Clostridium at the genus level [22] . Previous studies suggested that the properties of the intestinal microbiota in patients with UC were distinct from those in healthy individuals [23] . To further investigate RLP had a beneficial effect on the dysbiosis induced by canine ulcerative colitis, 16S rRNA sequencing data from fecal samples collected from different groups was analyzed.…”

“…[22] Previous studies suggested that the properties of the intestinal microbiota in patients with UC were distinct from those in healthy individuals. [23] To further investigate RLP had a beneficial effect on the dysbiosis induced by canine ulcerative colitis, 16S rRNA sequencing data from fecal samples collected from different groups was analyzed. The bacterial communities were tested by Venn diagram analysis (Figure 4A), which detailed the overlap and unique organisms between the OTUs in the fecal microbiota of each group.…”

Reduction of product composition variability using pooled microbiome ecosystem therapy and consequence in two infectious murine models

“…This profile, characterized by a high Proteobacteria/Firmicutes ratio, was also observed in mice receiving the least effective products from donors 1, 2, and 3 and positively correlates with colonic inflammation. Indeed, Proteobacteria, a phylum characterized by a high presence of endotoxin-bearing bacteria, is positively correlated with fecal calprotectin, an inflammation marker used in intestinal bowel disease (IBD) disease activity monitoring ( 68 ). Enrichment of bacterial genera associated with health benefits highlighted by a high Butycore index was also observed in mice treated with pooled product, while the results were heterogenous for mice treated with single donor-derived products.…”