Ehrlichiosis

Stubbs, Cynthia J.

doi:10.1016/b978-1-56053-461-7.50082-6

Cited by 11 publications

(18 citation statements)

References 7 publications

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“…These results are similar to those reported by Stubbs et al (2000) regarding age, although those authors found gender predominance with higher female positivity. According to our results, Ehrlichia infection is distributed equally in terms of age and sex, highlighting the widespread dissemination of the infection in the feline population treated at the Federal University of Mato Grosso Veterinary Hospital.…”

Section: Discussionsupporting

confidence: 65%

Hematological values associated to the serological and molecular diagnostic in cats suspected of Ehrlichia canisinfection

Braga

Santos

Melo

et al. 2013

Rev. Bras. Parasitol. Vet.

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The literature contains several studies on feline ehrlichiosis. However, information about the characteristics of Ehrlichia infection in cats is still scanty. This study evaluated the association between Ehrlichia spp. infection and the hematologic data of 93 cats treated at the Federal University of Mato Grosso Veterinary Hospital in Cuiabá, state of Mato Grosso, Brazil. The presence of or exposure to Ehrlichia spp. infection was evaluated by Polymerase Chain Reaction (PCR) targeting the dsb and 16S rRNA gene of Ehrlichia, and by detection of anti-Ehrlichia canis IgG antibodies in Indirect Fluorescence Assay (IFA), respectively. Eight (8.6%) cats tested positive by PCR and the partial DNA sequence obtained from PCR products was a 100% match to E. canis. Forty-two (45.1%) cats showed antibody reactivity against Ehrlichia spp. Hematological alterations such as low erythrocyte count, thrombocytopenia, lymphopenia and monocytosis were observed in PCR positive cats. Among them, low erythrocyte counts were associated with IgG antibody titers of 40 to 640 and five cats also tested positive by PCR. Furthermore, PCR-positive cats showed a tendency to be lymphopenic. No correlation was found between age and sex, and no ticks were observed in any of the examined cats.Keywords: Ehrlichiosis, feline, hematology, polymerase chain reaction, indirect fluorescence assay, diagnosis. ResumoDiversos estudos sobre erliquiose felina vêm sendo relatados na literatura. No entanto, a caracterização da infecção por Ehrlichia em gatos ainda é escassa. O presente estudo objetivou avaliar a associação entre infecção por Ehrlichia e dados hematológicos em 93 gatos atendidos no Hospital Veterinário da Universidade Federal de Mato Grosso, em Cuiabá, Brasil. A presença de infecção por Ehrlichia spp. foi avaliada pela Reação em Cadeia pela Polimerase (PCR) visando à amplificação dos genes dsb e 16S rRNA de Ehrlichia e por Reação de Imunofluorescência Indireta (RIFI). Oito (8,6%) gatos demonstraram ser positivos pela PCR, sendo suas sequências parciais de DNA 100% idênticas à E. canis. Quarenta e dois gatos (45,1%) apresentaram anticorpos reativos contra Ehrlichia spp. Alterações hematológicas como baixas contagens de eritrócitos, trombocitopenia, linfopenia e monocitose foram observadas em gatos positivos pela PCR. Dentre essas, eritropenia foi associada em gatos com títulos de anticorpos IgG entre 40 e 640, sendo cinco destes positivos pela PCR. Adicionalmente, gatos positivos na PCR apresentaram uma tendência a serem linfopênicos. Não foram observadas associações entre a presença de infecção nos gatos e suas respectivas idades e sexo. Nenhum carrapato foi observado nos gatos examinados.Palavras-chave: Erliquiose, felinos, hematologia, reação em cadeia pela Polimerase, reação de imunofluorescência indireta, diagnóstico.

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Section: Discussionsupporting

confidence: 65%

Hematological values associated to the serological and molecular diagnostic in cats suspected of Ehrlichia canisinfection

Braga

Santos

Melo

et al. 2013

Rev. Bras. Parasitol. Vet.

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“…has been infrequently detected in cats in others regions of the world, such as in the USA (Breitschwerdt et al, 2002), Taiwan (Yin-Chiachun et al, 2003) and Spain (Tabar et al, 2008). Feline ehrlichiosis has been associated with fever, apathy, anorexia, weight loss, palor, lymphadenopathy, splenomegaly, normocytic normochromic anemia, thrombocytopenia, leukopenia, increased serum activity of transaminases and alkaline phosphatase (Almosny et al, 1998;Almosny and Massard, 1999;Stubbs et al, 2000). Vectors involved in the transmission cycles of Ehrlichia spp.…”

Section: Discussionmentioning

confidence: 97%

“…On the other hand, vectors of feline ehrlichiosis and anaplasmosis, caused by Ehrlichia canis and Anaplasma phagocytophilum, respectively, are still unknown (Almosny et al, 1998;Almosny and Massard, 1999;Stubbs et al, 2000;Bjoersdorff et al, 1999;Tarello, 2005). Exposure to arthropods (ticks and fleas) and ingestion of rodents are suggestive routes of transmission of this disease among cats (Beaufils et al, 1999).…”

Section: Introductionmentioning

confidence: 97%

Tick-borne agents in domesticated and stray cats from the city of Campo Grande, state of Mato Grosso do Sul, midwestern Brazil

André

Herrera

Fernandes

et al. 2015

Ticks and Tick-borne Diseases

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“…Clinical signs and laboratory abnormalities observed in cats infected with E. canis include fever, lethargy, anorexia, pale mucous membranes, lymphadenopathy, splenomegaly, anemia, thrombocytopenia, leukopenia, increased serum activity of transaminases and alkaline phosphatase, and hypergammaglobulinemia (ALMOSNY & MASSARD, 1999;STUBBS et al, 2000).…”

mentioning

confidence: 99%

Ehrlichia spp. infection in domestic cats from Rio de Janeiro State, southeast Brazil

Guimarães

Raimundo

Rodrigues

et al. 2019

Rev. Bras. Parasitol. Vet.

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Ehrlichiosis is caused by agents belonging to Ehrlichia genus. Despite the frequent reports on the serological and molecular detection of E. canis in dogs in Brazil, there is scant data on ehrlichiosis in brazilian cats. This study aimed at investigating the occurrence of Ehrlichia spp. in domestic cats from Greater Rio de Janeiro, and evaluating hematological changes associated with this rickettsial infection. We searched for IgG antibodies against E. canis on blood samples of 216 cats by Indirect Fluorescence Assay (IFA). Additionally, we performed nested PCR (nPCR) and real-time PCR (qPCR) assays targeting E. canis-16S rRNA and dsb gene, respectively. Fifty-seven (26.4%) cats were seropositive for Ehrlichia spp. by IFA. Ehrlichia spp.-16S rRNA gene fragments were detected in 3 cats (1.4%). Although the obtained 16S rRNA sequences showed 99 to 100% identity with E. canis, cats were negative in qPCR. Anemia, thrombocytopenia, leukocytosis, left shift neutrophil and hyperproteinemia were observed. Anemia was statistically associated with seropositivity to E. canis and kittens showed lower positivity rates (p<0.05). This study showed that Ehrlichia spp. occur in domestic cats from Greater Rio de Janeiro. Further studies involving culture isolation are much needed to more precisely characterize these organisms. ResumoA erliquiose é causada por agentes pertencentes ao gênero Ehrlichia. Apesar dos frequentes relatos de detecção sorológica e molecular de E. canis em cães no Brasil, existem poucos dados sobre a erliquiose em gatos brasileiros. Este estudo teve como objetivo investigar a ocorrência de Ehrlichia spp. em gatos domésticos do Grande Rio de Janeiro e avaliar as alterações hematológicas associadas a essa infecção rickettsial. Procuramos anticorpos IgG anti-E. canis em amostras de sangue de 216 gatos por Reação de Imunofluorescência Indireta (RIFI). Além disso, foram realizados ensaios de nested PCR (nPCR) e PCR em tempo real (qPCR) para detecção dos genes E. canis-16S rRNA e dsb, respectivamente. Cinquenta e sete (26,4%) gatos foram soropositivos para Ehrlichia spp. pela RIFI. Fragmentos do gene rRNA de Ehrlichia spp.-16S foram detectados em 3 gatos (1,4%) por ensaios de nPCR. Embora as sequências 16S rRNA obtidas tenham 99 a 100% de identidade com E. canis, os gatos foram negativos nos ensaios de qPCR. Anemia, trombocitopenia, leucocitose, desvio nuclear neutrofílico à esquerda e hiperproteinemia foram observados. Anemia foi estatisticamente associada à soropositividade para E. canis e filhotes apresentaram menores taxas de positividade (p <0,05). Este estudo demonstra que Ehrlichia spp. ocorrem em gatos domésticos da Grande Rio de Janeiro. Outros estudos envolvendo o isolamento por cultura são necessários para caracterizar com mais precisão esses organismos.Palavras-chave: Felino, nested PCR, RIFI, hematologia, erliquiose.

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Ehrlichiosis

Cited by 11 publications

References 7 publications

Hematological values associated to the serological and molecular diagnostic in cats suspected of Ehrlichia canisinfection

Hematological values associated to the serological and molecular diagnostic in cats suspected of Ehrlichia canisinfection

Tick-borne agents in domesticated and stray cats from the city of Campo Grande, state of Mato Grosso do Sul, midwestern Brazil

Ehrlichia spp. infection in domestic cats from Rio de Janeiro State, southeast Brazil

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