Eicosanoid Synthesis by Purified Thrombocytes and Erythrocytes from Warm- and Cold-Acclimated American Bullfrogs (Rana catesbeiana)

Wang, Xinying; Herman, Ceil A.

doi:10.1006/gcen.1996.6864

Cited by 5 publications

(3 citation statements)

References 28 publications

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“…Upon activation of both nucleated thrombocytes and platelets, cellular aggregation, adhesion and morphological alterations are observed. Aggregation of both cell types can be induced by agents such as ADP and thrombin (Aledort, 1971;Haslam, 1964;O'Toole et al, 1994;Pica et al, 1990), and activation of both appears to involve an integrin-like surface protein complex and eicosanoid signaling pathways (Hill et al, 1999;Lloyd-Evans et al, 1994;Passer et al, 1997;Wang and Herman, 1997). For activated platelets, the morphological changes include membrane protrusions, pseudopod formation, and spreading on a surface (Allen et al, 1979;Zucker and Nachmias, 1985), and these activities are correlated primarily with the assembly of F-actin rather than MB reorganization (Debus et al, 1981;Nachmias, 1980).…”

Section: Introductionmentioning

confidence: 99%

Shape transformation and cytoskeletal reorganization in activated non‐mammalian thrombocytes

Lee

Miller

Anastassov

et al. 2004

Cell Biology International

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The nucleated thrombocytes of non-mammalian vertebrates are partially flattened, ovoid cells morphologically distinct from mammalian platelets, and the extent of their functional equivalence is unknown. To test whether they resemble platelets in having similar F-actin-based post-activation stages, rapid fixation/extraction/labeling methods were developed to reveal cytoskeletal organization in dogfish thrombocytes by confocal microscopy. Unactivated cells contained cortical F-actin plus denser F-actin co-localizing with outer marginal band (MB) microtubules. In the post-activation sequence, determined for the first time by continuous observation of individual thrombocytes following thrombin perfusion, cells rounded and blebbed, spread, and eventually flattened extensively. The MB twisted and then became disorganized, with microtubule bundles remaining centrally located and associated with nuclear clefts. In contrast, F-actin occupied blebs and outward-spreading cytoplasm, initially in spiky projections, then predominantly in stress fibers, and inhibitors of F-actin assembly or myosin ATPase blocked shape changes. Thus, the post-activation stages and cytoskeletal events observed in nucleated thrombocytes were found to parallel those of platelets.

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Section: Introductionmentioning

confidence: 99%

Shape transformation and cytoskeletal reorganization in activated non‐mammalian thrombocytes

Lee

Miller

Anastassov

et al. 2004

Cell Biology International

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“…Co-94700 has the motif C-x(14,17)-W-x(2)-W-x(9,11)-C-x(3)-C-x(52,56)-C found in lipid-binding D7 proteins. Frog thrombocytes produce cysteinyl leukotrienes (Gronert et al, 1995; Wang and Herman, 1997), and these could be targeted by this D7 protein. The motif C-E-x(14,17)-R-x-Y-x(10)-H-x(2)-C-x(55,58)-Y-x(15)-F-D-x(2)-E recognizes mosquito serotonin binding proteins but does not identify any C. appendiculata protein from our transcriptome analysis.…”

Section: Resultsmentioning

confidence: 99%

“…Indeed there are 155,994 PubMed articles retrieved with the words “platelet” and “human,” but only 104 with the words “thrombocyte” and “amphibian”. Nonetheless, Rana thrombocytes are known to produce thromboxane and cysteinyl-leukotrienes (Gronert et al, 1995; Stiller et al, 1974; Wang and Herman, 1997). Turtle thrombocytes were shown to have serotonin (Maurer-Spurej, 2005), and snake and fish thrombocytes are aggregated by ADP (Gregory and Jagadeeswaran, 2002; Sanomartins et al, 1994), indicating the conservation of the main agonists of platelet aggregation and vasoconstriction between fish, amphibians, reptiles, and mammals.…”

Section: Discussionmentioning

confidence: 99%

An insight into the sialome of the frog biting fly, Corethrella appendiculata

Ribeiro

Chagas

Pham

et al. 2014

Insect Biochemistry and Molecular Biology

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The Nematocera infraorder Culicomorpha is believed to have descended from bloodfeeding ancestors over 200 million years ago, generating bloodfeeding and non-bloodfeeding flies in two superfamilies, the Culicoidea—containing the mosquitoes, the frog-feeding midges, the Chaoboridae, and the Dixidae—and the Chironomoidea—containing the black flies, the ceratopogonids, the Chironomidae, and the Thaumaleidae. Bloodfeeding requires many adaptations, including development of a sophisticated salivary potion that disarms host hemostasis, the physiologic mechanism comprising platelet aggregation, vasoconstriction, and blood clotting. The composition of the sialome (from the Greek sialo = saliva) from bloodfeeding animals can be inferred from analysis of their salivary gland transcriptome. While members of the mosquitoes, black flies, and biting midges have provided sialotranscriptome descriptions, no species of the frog-biting midges has been thus analyzed. We describe in this work the sialotranscriptome of Corethrella appendiculata, revealing a complex potion of enzymes, classical nematoceran protein families involved in bloodfeeding, and novel protein families unique to this species of frog-feeding fly. Bacterial (Wolbachia) and novel viral sequences were also discovered.

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