Eight-Year Survey of Human Rotavirus Strains Demonstrates Circulation of Unusual G and P Types in Hungary

Bányai, Krisztiàn; Gentsch, Jon R.; Glass, Roger I.; M, Uj; Mihály, Ilona; Szücs, György

doi:10.1128/jcm.42.1.393-397.2004

Cited by 51 publications

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“…Although a reduction in the episodes of gastroenteritis attributable to rotaviruses was seen between 2005 and 2008, it is not clear whether the recent introduction of rotavirus testing and sampling for the current study may be a confounding factor, and only continued surveillance will allow the monitoring of this trend in the long term. [17,18,23,[37][38][39][40][41]. These fluctuations may be driven by herd immunity and the emergence of a particular genotype may reflect the buildup of a sufficiently large population of susceptible children.…”

Section: The Burden Of Rotavirus Diseasementioning

confidence: 99%

“…However, geographical and temporal differences exist in the distribution of G and P types and their combinations: P [6] in association with G1, G2, G3, and G9 and G8 in combination with P [8] and P [6] have been shown to be the second most detected P and G type in India and some parts of the African continent, respectively [11][12][13][14][15][16]. Recent studies have reported the emergence of uncommon G or P types (G5, G6, G10, G11, G12, P [3], P [7], P [9], P [11], and many more) in different countries [10,11,[17][18][19][20][21][22]. These uncommon rotavirus G/P genotypes emerge in humans through zoonotic transmission, and reassortment with common human rotavirus strains can potentially lead to the emergence of a pandemic strain [23].…”

Section: Introductionmentioning

confidence: 99%

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Molecular epidemiology of rotaviruses in Bulgaria: annual shift of the predominant genotype

Mladenova

Korsun²,

Geonova³

et al. 2010

Eur J Clin Microbiol Infect Dis

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Section: The Burden Of Rotavirus Diseasementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Molecular epidemiology of rotaviruses in Bulgaria: annual shift of the predominant genotype

Mladenova

Korsun²,

Geonova³

et al. 2010

Eur J Clin Microbiol Infect Dis

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“…Most of these specificities can also be found in animals, suggesting that certain human G and P types, especially those that are rarely detected, might have originated from animal strains either by direct interspecies transmission or by reassortment of cognate genes between heterologous and homologous strains. Serotypes G6 and G8, identified frequently in ruminants, or serotype G5, common in pigs but rare in humans, exemplify this hypothesis (2,4,6,11). Although direct epidemiological evidence is still lacking, studies based on full genome hybridization and, more recently, on sequence and phylogenetic analysis seem to support the hypothesis that interspecies transmission may occur (4,13,29), even though such analyses usually cannot demonstrate direct evolutionary ancestry between recent animal and human strains.…”

mentioning

confidence: 99%

“…To date, P [6] strains have been detected from both asymptomatic neonatal infections and symptomatic infantile infections in association with a wide variety of G types (5,6,13,18,29). At present there is no epidemiological data on neonatal asymptomatic infections in Hungary; however, genotype P [6] strains, in linkage with G4 specificity, have been sporadically identified from episodes of gastroenteritis of infants and young children (2).…”

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confidence: 99%

Sequencing and Phylogenetic Analysis of Human Genotype P[6] Rotavirus Strains Detected in Hungary Provides Evidence for Genetic Heterogeneity within the P[6] VP4 Gene

et al. 2004

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Although rotavirus genotype P[6] is one of the three most common VP4 specificities associated with human infection, the relatively few sequence data available in public databases suggest that the genetic variability within P[6] might be presently unexplored. Thus far, two human P[6] lineages (M37-like and AU19-like) and a single porcine P[6] lineage (Gottfried-like) have been identified by phylogenetic analysis. Serologic studies demonstrated that these three lineages are antigenically distinct from each other, a finding based on which they were classified into three subtypes, P2A[6] (M37-like), P2B[6] (Gottfried-like), and P2C[6] (AU19-like). To study heterogeneity within this genotype, we selected for molecular characterization a total of six P[6] strains detected during an ongoing surveillance in Hungary. The variable region of the VP4 gene was subjected to sequencing and phylogenetic analysis. Our data indicated that these six strains fell into two phylogenetic lineages distinguishable from the human lineages M37-like and AU19-like and from the porcine lineage Gottfried-like. Further studies are needed to understand whether these two novel lineages are genuine human strains or might have originated from animal strains and to evaluate the antigenic relationship of the novel Hungarian P[6] strains to the three established subtypes.Group A rotaviruses, members of the family Reoviridae, are the major cause of acute dehydrating gastroenteritis in children and young animals (7). The viral genome composed of 11 segments of double-stranded RNA is enclosed in a nonenveloped, icosahedral, triple-layered capsid. The outer capsid antigens, VP7 and VP4, carry the serotype and the genotype specificities and serve as a basis for the dual nomenclature. The specificities of VP7 are designated with a G (VP7 is a glycoprotein), while the specificities of VP4 are designated with a P (VP4 is a protease-sensitive protein). Based on nucleotide and deduced amino acid sequences, at least 15 G genotypes and 23 P genotypes have been established. Due to the lack of adequate immunological reagents (i.e., type-specific monoclonal antibodies or hyperimmune sera), some of the P genotypes have not been characterized serologically and a dual classification by P serotypes (designated with unbracketed numbers) and P genotypes (designated with bracketed numbers) is presently used for VP4 characterization. Out of 23 different P genotypes, only 14 P serotypes and 3 subtypes have been identified (7,14,21,23).Thus far, at least 10 G types (G1 to G6, G8 to G10, and G12) and 11 P types (P[1], P[3] to P[6], P[8] to P[11], P[14], and P[19]) have been identified in humans. Most of these specificities can also be found in animals, suggesting that certain human G and P types, especially those that are rarely detected, might have originated from animal strains either by direct interspecies transmission or by reassortment of cognate genes between heterologous and homologous strains. Serotypes G6 and G8, identified frequently in ruminants, or serotype G5, co...

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“…For VP4 genotyping, second amplification was done with specific primers to VP4 genotypes P [4], P2A [6], P [8], P [9] and P [10] (14); P [1], P [5], P2B [6], P [7] and P [11] (19).…”

Section: Introductionmentioning

confidence: 99%

Serological and molecular diversity of human rotavirus in São Paulo, Brazil

Munford

Souza

Caruzo

et al. 2007

Braz. J. Microbiol.

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From a total of 187 fecal samples from children with ages between 0 and 5 years, collected in the Hospital Universitário -USP, Brazil, from 1994 to 1996, 54 (28.9%) were positive for rotavirus. Positive samples were characterized by electropherotyping, subgrouping, G serotype and genotype and P genotype. Rotavirus electropherotypes were characterized in four different long genome patterns (38.9%), one short genome pattern (34.0%) and 18.0% were characterized as an unusual pattern. Subgroup I was found in 38.9% strains, subgroup II in 50.0% and 7.7% was subgroup nonI-nonII. For G serotypes, G2 was found in 59.3%, G1 was identified in 33.3% of strains, two samples showed mixtures of G1+G2 and one sample was G1+G3. Ten samples characterized as serotype G2 showed a long eletropherotype. Genotype G2 was the most frequently and was found in 37 (44.0%) samples (23 samples as a single genotype and 14 as mixtures of genotypes). G1 was found in 15 samples. G3 and G4 was detected mainly in mixtures of genotypes and G5, G6 and G9 were identified only in mixtures. A total of 20 (38.5%) samples were characterized as G genotype mixtures and P mixtures were found in 16 (29.6%) samples. P [4] was found in 55.6% of samples, P[8] in 51.9% and P[6-M37 like] in 22.3% of cases. P[6-Gottfried like] and P[11] were detected only in mixtures. One sample with G6 specificity, mixed with a G2 rotavirus and a P[11] strain, mixed with P[4] and P[8]strain was described for the first time in Latin America.

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Eight-Year Survey of Human Rotavirus Strains Demonstrates Circulation of Unusual G and P Types in Hungary

Cited by 51 publications

References 45 publications

Molecular epidemiology of rotaviruses in Bulgaria: annual shift of the predominant genotype

Molecular epidemiology of rotaviruses in Bulgaria: annual shift of the predominant genotype

Sequencing and Phylogenetic Analysis of Human Genotype P[6] Rotavirus Strains Detected in Hungary Provides Evidence for Genetic Heterogeneity within the P[6] VP4 Gene

Serological and molecular diversity of human rotavirus in São Paulo, Brazil

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