Eighteen New Candidate Effectors of the Phytonematode <i>Heterodera glycines</i> Produced Specifically in the Secretory Esophageal Gland Cells During Parasitism

Noon, Jason B.; Hewezi, Tarek; Maier, Thomas R.; Simmons, Carl R.; Wei, Jun‐Zhi; Wu, Guangyu; Llaca, Víctor; Deschamps, Stéphane; Davis, Eric; Mitchum, Melissa G.; Hussey, R. S.; Baum, Thomas J.

doi:10.1094/phyto-02-15-0049-r

Cited by 58 publications

(108 citation statements)

References 70 publications

Supporting

Mentioning

107

Contrasting

Order By: Relevance

“…A). The dorsal gland localization of GLAND4 is consistent with previous findings in H. glycines (Noon etal ., ).…”

Section: Resultsmentioning

confidence: 97%

“…GLAND4 is one of many pioneer effectors produced by cyst nematodes that lack significant global alignment to known proteins, and, as such, protein structure and function prediction tools cannot provide reliable insights into the function of GLAND4 (Gao etal ., , Noon etal ., ; Wang etal ., ). Local alignment of GLAND4 to a histone‐like repeat region alongside the confirmation of nuclear localization, performed as part of this study, provided a reason to test the hypothesis that GLAND4 functions as a DNA‐binding protein.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

The plant‐parasitic cyst nematode effector GLAND4 is a DNA‐binding protein

Barnes

Wram

Mitchum

et al. 2018

Molecular Plant Pathology

Self Cite

View full text Add to dashboard Cite

Cyst nematodes are plant pathogens that infect a wide range of economically important crops. One parasitic mechanism employed by cyst nematodes is the production and in planta delivery of effector proteins to modify plant cells and suppress defences to favour parasitism. This study focuses on GLAND4, an effector of Heterodera glycines and H. schachtii, the soybean and sugar beet cyst nematodes, respectively. We show that GLAND4 is recognized by the plant cellular machinery and is transported to the plant nucleus, an organelle for which little is known about plant nematode effector functions. We show that GLAND4 has DNA-binding ability and represses reporter gene expression in a plant transcriptional assay. One DNA fragment that binds to GLAND4 is localized in an Arabidopsis chromosomal region associated with the promoters of two lipid transfer protein genes (LTP). These LTPs have known defence functions and are down-regulated in the nematode feeding site. When expressed in Arabidopsis, the presence of GLAND4 causes the down-regulation of the two LTP genes in question, which is also associated with increased susceptibility to the plant-pathogenic bacterium Pseudomonas syringae. Furthermore, overexpression of one of the LTP genes reduces plant susceptibility to H. schachtii and P. syringae, confirming that LTP repression probably suppresses plant defences. This study makes GLAND4 one of a small subset of characterized plant nematode nuclear effectors and identifies GLAND4 as the first DNA-binding, plant-parasitic nematode effector.

show abstract

“…A). The dorsal gland localization of GLAND4 is consistent with previous findings in H. glycines (Noon etal ., ).…”

Section: Resultsmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

The plant‐parasitic cyst nematode effector GLAND4 is a DNA‐binding protein

Barnes

Wram

Mitchum

et al. 2018

Molecular Plant Pathology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Consistent with this idea, protease-like effectors have been identified in M. incognita (Neveu et al, 2003;Danchin et al, 2013), G. pallida (Jones et al, 2009), H. glycines (Noon et al, 2015), and Meloidogyne chitwoodi (Roze et al, 2008). Phytonematodes not only secrete protease-like effectors but also target host proteases.…”

Section: Proteolysismentioning

confidence: 83%

Cellular Signaling Pathways and Posttranslational Modifications Mediated by Nematode Effector Proteins

Hewezi

2015

Plant Physiol.

Self Cite

View full text Add to dashboard Cite

Plant-parasitic cyst and root-knot nematodes synthesize and secrete a suite of effector proteins into infected host cells and tissues. These effectors are the major virulence determinants mediating the transformation of normal root cells into specialized feeding structures. Compelling evidence indicates that these effectors directly hijack or manipulate refined host physiological processes to promote the successful parasitism of host plants. Here, we provide an update on recent progress in elucidating the molecular functions of nematode effectors. In particular, we emphasize how nematode effectors modify plant cell wall structure, mimic the activity of host proteins, alter auxin signaling, and subvert defense signaling and immune responses. In addition, we discuss the emerging evidence suggesting that nematode effectors target and recruit various components of host posttranslational machinery in order to perturb the host signaling networks required for immunity and to regulate their own activity and subcellular localization.The root-knot (Meloidogyne spp.) and cyst (Globodera and Heterodera spp.) nematodes are sedentary endoparasites of the root system in a wide range of plant species. These obligate parasites engage in intricate relationships with their host plants that result in the transformation of normal root cells into specialized feeding sites, which provide the nematodes with all the

show abstract

“…It has been generally found that, in geographic regions that SCN-resistant cultivars have been grown the longest, field isolates increase in their likelihood in overcoming resistance in cultivars (Faghihi et al, 2010). More recently, effector candidates have been identified from SCN (Noon et al, 2015), but their roles in parasitism remain to be discovered. More recently, effector candidates have been identified from SCN (Noon et al, 2015), but their roles in parasitism remain to be discovered.…”

Section: Impact Of Soybean Cyst Nematode Resistance On Soybean Yieldmentioning

confidence: 99%

Impact of Soybean Cyst Nematode Resistance on Soybean Yield

2017

View full text Add to dashboard Cite

Soybean cyst nematode (SCN) (Heterodera glycines Ichinohe) of soybean [Glycine max (L.) Merr.] causes extensive yield loss, and host resistance has been an effective strategy to minimize this loss. However, shifts in SCN population virulence compatibility have resulted from the extensive use of PI 88788 as a source of resistance in the northern United States. The Northern Regional Soybean Cyst Nematode Tests offer a vast amount of yield testing, combined with resistance screening of entries and the characterization of the SCN infection of test fields. The objectives of this study were to use regional test results (i) to quantify the impact of resistance as the initial field SCN egg counts increase, (ii) to explore effects of maturity group (MG) on resistance, and (iii) to gain insights into how the ability of SCN to infect germplasm with resistance from PI 88788 affects yield. Yield tests across 11 yr were combined into a single dataset with over 1247 test–environment combinations. Analysis of these data showed that the yield advantage of SCN resistant entries increased as initial egg counts increased, and a larger advantage was found in early MGs (00–II) than in later MGs (III–IV). A yield advantage was documented at environments with an initial egg count as low as 100 eggs 100 cm−3 soil. At the levels of SCN infection on PI 88788 found in infested locations, breeding lines with resistance from PI 88788 yielded more than susceptible entries. Analysis of this dataset offers a unique view of the impact SCN resistance provides to soybean.

show abstract

Eighteen New Candidate Effectors of the Phytonematode Heterodera glycines Produced Specifically in the Secretory Esophageal Gland Cells During Parasitism

Cited by 58 publications

References 70 publications

The plant‐parasitic cyst nematode effector GLAND4 is a DNA‐binding protein

The plant‐parasitic cyst nematode effector GLAND4 is a DNA‐binding protein

Cellular Signaling Pathways and Posttranslational Modifications Mediated by Nematode Effector Proteins

Impact of Soybean Cyst Nematode Resistance on Soybean Yield

Contact Info

Product

Resources

About