Eine neue Methode zur Ex-vivo-Vollblut-Perfusion isolierter Warmblüterorgane, dargestellt an der Niere von Schweinen - A New Method for ex vivo Whole-blood Perfusion of Isolated Organs of Large Mammals Demonstrated in Pig Kidneys

Baeyer, Hans von; Stahl, Katja; Häusler, M; Meissler, M.; Unger, Volker; Frank, Jacklyn; Große-Siestrup, Ch.; Kaczmarczyk, Gabriele; Affeld, K.; Flaig, H.-J.; Steinbach, Bernd

doi:10.1515/bmte.1997.42.3.61

Cited by 11 publications

(1 citation statement)

References 3 publications

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“…Many analyses can be performed in small volume samples, but larger amounts of blood may be desired for particular studies, for example, functional neutralization assays, in vitro flow-chamber model systems or ex vivo kidney perfusion studies. 3,4 Collection of larger quantities of blood, however, can be troublesome as it often requires access to deeper veins, which mandates surgical exposure of the vessel, requiring general anaesthesia and heparinization to prevent coagulation. The latter may not always be desirable as heparin content in blood samples may affect analysis of key functions of the blood, including coagulation propensity, complement 5 and contact activation system.…”

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A method for collecting high numbers of blood samples in standard vacuum tubes from non-heparinized pigs

et al. 2022

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Large animal models allow for collection of substantial amounts of biological material. However, the collection of larger volumes (>100 ml) of blood from pigs can be a challenge: (i) the peripheral veins are not suitable for collection of high numbers of standard blood tubes as the veins tend to collapse; and (ii) the alternative option of cannulating deeper veins mandates surgical exposure of the vessels and often the need for heparinization, which is undesirable for some blood analysis. During an immunization trial in 40 pigs, we assessed the femoral and saphenous arteries as practical vessels for collecting 250 ml of blood from each pig in standard collection tubes without heparinization. Blood collected from the saphenous artery by a standard butterfly needle proved particularly useful and 250 ml blood could be collected successfully in 24 of 25 pigs by this approach.

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A method for collecting high numbers of blood samples in standard vacuum tubes from non-heparinized pigs

et al. 2022

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Improved Routine Bio-Medical and Bio-Analytical Online Fluorescence Measurements Using Fluorescence Lifetime Resolution

Stein¹,

Fink²,

Welker

et al. 2005

J Fluoresc

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Fluorescence techniques are widely used as sensitive detection methods in bio-analytics. The use of the bio-physical parameter fluorescence lifetime additional to the spectral characteristics of fluorescence has the potential to improve fluorescence-related detection methods in terms of selectivity in signal recognition, robustness against disturbing influences, and the accessibility of novel bio-chemical process parameters. This article describes the technical set up of a time-resolving instrument with either a fixed time-gated detection principle for improved evaluation of tissue metabolism by an online monitoring of the tissue autofluorescence or a direct fluorescence lifetime detection principle for lifetime-based fluorescent assays.

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Functional Effects of Acute Coronary Occlusion and Catecholinergic Stimuli on the Isolated Normothermic Hemoperfused Porcine Heart

Nogueira

Ast²,

Patone³

et al. 2003

Clinical and Experimental Hypertension

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The aim of this study was to characterize functional parameters in the isolated and normothermic hemoperfused porcine beating heart model after pathophysiological stimuli for extended perfusion periods. Hearts were prepared and connected to a specially developed perfusion equipment, which simultaneously allowed perfusion with warm autologous blood as well as blood dialysis. Two groups were established: group A (12 hearts: no intervention) and group B (6 hearts: occlusion of the ramus circumflexus of the left coronary artery for 2 hours). Blood gas analyses and oxymetry were performed at baseline and every 30 min during a 6 hours perfusion period. Coronary perfusion pressure (CPP) and blood flow (CBF), right and left ventricular pressure, blood and dialyzate pH-values, and temperature were monitored online by a microcontroller. A steady state regarding the CPP and the CBF was achieved after 1 hour of perfusion for both groups. In group B, CPP increased during occlusion. Comparison of both groups showed no significant differences in the bicarbonate and sodium levels in blood and dialyzate. The potassium concentration in blood and dialyzate increased in both groups constantly during the experiments. No clear alteration of the oxygen consumption was observed. Lactate levels in blood and dialyzate increased during occlusion as did the aspartataminotransferase (AST) venous levels (both determined only for group B). Four concentrations of norepinephrine were injected into the stem of the coronary arteries (10, 20, 40, 80 microg). A clear inotropic effect of this hormone on right and left ventricular pressure was observed. It was concluded that longer perfusion periods and simulation of myocardial infarction for a clinically relevant period can be performed using this model. In addition, right and left ventricular function appear to be well preserved in this model, since the isolated porcine heart responded to norepinephrine stimuli.

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Eine neue Methode zur Ex-vivo-Vollblut-Perfusion isolierter Warmblüterorgane, dargestellt an der Niere von Schweinen - A New Method for ex vivo Whole-blood Perfusion of Isolated Organs of Large Mammals Demonstrated in Pig Kidneys

Cited by 11 publications

References 3 publications

A method for collecting high numbers of blood samples in standard vacuum tubes from non-heparinized pigs

A method for collecting high numbers of blood samples in standard vacuum tubes from non-heparinized pigs

Improved Routine Bio-Medical and Bio-Analytical Online Fluorescence Measurements Using Fluorescence Lifetime Resolution

Functional Effects of Acute Coronary Occlusion and Catecholinergic Stimuli on the Isolated Normothermic Hemoperfused Porcine Heart

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