Einfache Methode zum Nachweis mikrobieller Lipase

Wand, Handan; Gabert, A; Uhlig, H.

doi:10.1002/jobm.3630200409

Z Allg Mikrobiol

1980

DOI: 10.1002/jobm.3630200409

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Einfache Methode zum Nachweis mikrobieller Lipase

Handan Wand¹,

A Gabert²,

H. Uhlig³

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1987

1993

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Cited by 4 publications

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“…Screening of lipase producers on agar plates is frequently done by using tributyrin (9) or Tween 80 (12) as a substrate. Modifications of these assays use Tween 80 in combination with Nile-blue (7) or neat's-foot oil, with Cu2+ salts (13). These substrates are not suitable to detect true lipases because they are hydrolyzed by esterases, too.…”

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confidence: 99%

Specific and sensitive plate assay for bacterial lipases

Kouker¹,

Jaeger²

1987

Appl Environ Microbiol

615

254

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A plate assay to detect bacterial lipase (EC 3.1.1.3) in a medium containing trioleoylglycerol and the fluorescent dye rhodamine B is presented. Substrate hydrolysis causes the formation of orange fluorescent halos around bacterial colonies visible upon UV irradiation. The logarithm of lipase activity from cell-free culture supernatants is linearly correlated with the diameter of halos, thereby allowing quantitation of lipase activities ranging from 1 to 30 nkat.

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mentioning

confidence: 99%

Specific and sensitive plate assay for bacterial lipases

Kouker¹,

Jaeger²

1987

Appl Environ Microbiol

615

254

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Übersichtsartikel. Die Charakterisierung mikrobieller Lipasen. 1. Mitt. Zur Bestimmung der Lipaseaktivität

Bariszlovich

Meusel

Tülsner

1990

Nahrung

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In the selection of an appropriate method for activity determination of lipases existing technical equipment, kind of enzymes, number of samples investigated (e.g. in routine analysis), and expected sensitivity range have to be taken into account. Titrimetric methods and above all copper salt methods with their high detection sensitivity are the most suitable procedures for activity determination of lipases used in laboratories and institutions without equipment for radiochemical analysis.

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Fusarium solani: Invader of the ophidian eggs ofElaphe guttata in captivity

1993

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Einfache Methode zum Nachweis mikrobieller Lipase

Cited by 4 publications

References 1 publication

Specific and sensitive plate assay for bacterial lipases

Specific and sensitive plate assay for bacterial lipases

Übersichtsartikel. Die Charakterisierung mikrobieller Lipasen. 1. Mitt. Zur Bestimmung der Lipaseaktivität

Fusarium solani: Invader of the ophidian eggs ofElaphe guttata in captivity

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