Ekspresi Glukosa Transporter-2 di Sel Beta Pankreas dan Sel Hepatosit Tikus yang Diinduksi Diabetes Mellitus

Abstract

Diabetes Mellitus (DM) is an incurable disease that can be prevented clinically from worsening. This disease is based on classification related to pancreatic damage in total or partial so that the impact on insulin production. Glucose transporter is a protein that can glucose uptake from outside cell into cell and provides an important role in the body's glucose homeostasis. Glucose transporter-2 (GLUT-2) is found in the pancreas, liver, and kidney. Parameters used are the expression and amount of GLUT-2 density in pancreatic β cells and in hepatocyte cells in normal and diabetic Rat. Used Wistar strain male white rats. An immunohistochemistry (IHC) staining technique was performed to observe the expression of GLUT-2 and the macbiophotonics image J program to determine total density of GLUT-2. GLUT-2 is marked in brown in the cell nucleus surrounded by cytoplasm. The results showed an increased in GLUT-2 expression in hepatocyte cells with a total density of 25,447,052 and a decreased in GLUT-2 expression in pancreatic β cells with a total density of 5,074,544 by Streptozotocin 50 mg/kg BB-Nicotinamide 100 mg/kg BB (STZ-NA) Intraperitonial routes in induced DM rat. STZ-NA induced triggers damage in pancreatic β cells and significantly influences the expression of GLUT-2 protein. High blood glucose levels give different GLUT-2 activity in hepatocyte cells due to the effects of induced agent.

Exploration of the Potential of Dandang Gendis Leaf Extract (Clinacanthus nutans L) as an In Vitro Antidiabetic (A-Glucosidase Enzyme Inhibition Test) and In Vivo (Decrease Glucose Levels and Glut-2 Expression in Mice)

“…DGLE Potential in Enhancing GLUT-2 Expression GLUT-2 in healthy mice functions to transport blood sugar in mice and functions in the process of insulin secretion in the body to stabilize the regulation of blood sugar in mice [3]. Alloxan induction in mice can cause damage to pancreatic cells and cause insulin secretion to decrease [17], so they are unable to transmit signals to attract GLUT-2 to the active site on the cell membrane and resulting in decreased GLUT-2 activity [18]. GLUT-2 remains in the cell membrane but cannot be active because there is high oxidative stress which causes islet cell damage, thus interfering with insulin secretion and resulting in the mice's blood sugar being unable to be metabolized, resulting in hyperglycemia.…”

Peran Ekstrak Etanolik Daun Kelor (Moringa oleifera, Lam.) Terhadap Peningkatan Ekspresi GLUT-2 Pankreas Tikus Wistar (Rattus Norvegicus) Jantan Model Sindrom Metabolik Terinduksi

“…Terdapat penelitian lain oleh Hidayaturrahmah (2021) yang membuktikan pemberian diet tinggi lemak dan injeksi STZ-NA mampu menurunkan luas pulau Langerhans. Hasil ini telah dibuktikan dengan penelitian yang menyatakan induksi STZ-NA mampu menurunkan ekspresi GLUT-2 jaringan pankreas tikus dengan merusak sel β (Teodhora et al, 2021).…”