EKSTRAKSI DAN PURIFIKASI FUKOSANTIN DARI RUMPUT LAUT COKELAT Sargassum sp. SEBAGAI ANTIOKSIDAN

Sodik, Vitra; Tamat, Swasono R.; Suwarno, Tisno; Noviendri, Dedi

doi:10.34011/juriskesbdg.v14i1.2057

Cited by 4 publications

(5 citation statements)

References 9 publications

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“…Moreover, fucoxanthin significantly reduced the expression levels of genes encoding apoptotic proteins, such as caspase-3 and Bax (Figure 6C). These results are consistent with previous results obtained by other authors, including Dhami et al [59], Nisa et al [60], and Sodik et al [61].…”

Section: Fucoxanthin Exhibits a Neuroprotective Effect By Regulating ...supporting

confidence: 94%

“…Here, we found that the radical scavenging activity of fucoxanthin ( Table 5 ) was higher (8.34–31.80%) than that from S. fusiforme (24.3%), S. marginatum (11%), S. myriocystum (10–25%), and S. pallidum (29.4%) [ 68 , 69 , 70 ]. According to previous reports by Nisa et al [ 60 ] and Sodik et al [ 61 ], the antioxidant activity of fucoxanthin extracted from S. filipendula and Sargassum sp. was assessed by the DPPH method, with IC 50 values of 0.639 and 0.087 mg mL −1 , respectively, compared with our data in this study (IC 50 value of 3.42 mg mL −1 ; Table 5 ).…”

Section: Discussionmentioning

confidence: 87%

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Isolation of Fucoxanthin from Sargassum oligocystum Montagne, 1845 Seaweed in Vietnam and Its Neuroprotective Activity

Hong,

Thom,

et al. 2023

Biomedicines

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Fucoxanthin extracted and purified from Vietnamese Sargassum oligocystum Montagne, 1845 exhibits various biological activities. In this study, the ability of fucoxanthin to inhibit acetylcholinesterase (AChE), the antioxidant activities, and the expression of antioxidant enzymes were investigated. Fucoxanthin isolated from Vietnamese S. oligocystum showed no cytotoxic effects; moreover, it exhibited AChE inhibitory activity (with an IC50 value of 130.12 ± 6.65 μg mL−1) and antioxidant activity (with an IC50 value of 3.42 ± 0.15 mg mL−1). At concentrations of 50 and 100 µg mL−1, fucoxanthin provided protection against amyloid β-protein fragment 25–35-induced neurotoxicity in a C6 neuronal cell line, and the survival of C6 cells was higher than 81.01% and 80.98%, respectively, compared to the control group (59%). Moreover, antioxidant enzyme activity and quantitative PCR analysis suggested that the neuroprotective effect of fucoxanthin resulted from regulation of the gene expression of antioxidant enzymes (CAT and GPx) and ER pathways (caspase-3 and Bax), as well as the promotion of expression of genes involved in PI3K/Akt signaling (GSK-3β), autophagy (p62 and ATG5), and the biosynthesis of ACh (VAChT and ChAT). Therefore, fucoxanthin extracted from the seaweed S. oligocystum in Vietnam is a potential feedstock source for the production of health foods that exert neuroprotective effects.

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Section: Fucoxanthin Exhibits a Neuroprotective Effect By Regulating ...supporting

confidence: 94%

Section: Discussionmentioning

confidence: 87%

Isolation of Fucoxanthin from Sargassum oligocystum Montagne, 1845 Seaweed in Vietnam and Its Neuroprotective Activity

Hong,

Thom,

et al. 2023

Biomedicines

View full text Add to dashboard Cite

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“…Morever, we observated that FS signi cantly reduced expression levels of gene encording apoptotic protein like caspase -3 and Bax (Figure 7C). These results are completely consistent with the previous results of other authors (Dhami et al 2021;Nisa et al 2020;Sodik et al 2022).…”

Section: Neuroprotective Effects Of Fucoxanthin By Regulating Genes P...supporting

confidence: 94%

Elucidation of Antioxidant and Neuroprotective Potential of Fucoxanthin Isolated from Brown Seaweed Sargassum oligocystum

Thom

Thu

et al. 2023

Preprint

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Fucoxanthin belonging to the carotenoid family has different bioactive properties as anti-oxidant, anti-obesity, anti-diabetic, and anti-cancer effects. This study presents the results of the extraction and purification of fucoxanthin from Sargassum olygocystum Montagne, 1845 using thin-layer chromatography, column chromatography, and high-performance liquid chromatography. Furthermore, this study demonstrated the antioxidant activities, expression of antioxidant enzymes, and inhibition of acetylcholinesterase (AChE) of fucoxanthin. The chemical structure, molecular formula (C42H58O6), and molecular weight (658) of fucoxanthin were determined by nuclear magnetic resonance spectroscopy. Fucoxanthin isolated from S. olygocystum showed no cytotoxic effects, moreover, it showed potent antioxidant activity as assessed by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method (with an IC50 value of 3.42 ± 0.15 mg mL− 1), and AchE inhibitory activity (with an IC50 value of 130.12 ± 6.65 µg mL− 1). At concentrations of 50 and 100 µg mL− 1, fucoxanthin protected against amyloid β-protein fragment 25–35 - induced neurotoxicity on the C6 neuronal cell line with the survival of C6 cells higher than 81.01 and 80.98%, respectively, compared to the control group (59%). Moreover, antioxidant enzyme activity and quantitative PCR analysis revealed that the neuroprotective effect of fucoxanthin, was possibly due to not only regulation of gene expression of antioxidant enzyme (CAT and GPx), ER pathway (caspase-3 and Bax) but also promoting expression of genes involved in PI3K/Akt signaling (GSK-3β), autophagy (p62 and ATG5) and the biosynthesis of ACh (VAChT and ChAT). Therefore, fucoxanthin extracted from the Sargassum olygocystum is a potential feedstock source for the production of health foods with neuroprotective effects.

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“…Sargassum sp. mengandung klorofil serta golongan kartenoid polar (xantofil) dan non polar (karoten) (Limantara & Heriyanto, 2012) merupakan salah satu jenis rumput laut coklat yang memiliki banyak aktivitas biologis antara lain sebagai antioksidan, antiviral, antikanker, antibakteri maupun sebagai imunomodulator (Biradar, 2010;de Los Reyes Arguelles & Sapin, 2020;Kordjazi et al, 2019;Namvar et al, 2014;Noviendri et al, 2021;Sodik et al, 2022;Stiger-Pouvreau et al, 2023).…”

Section: Pendahuluanunclassified

Optimasi Metode dan Penentuan Batas Deteksi untuk Analisis Kadar Fukosantin dalam Sargassum ilicifolium (Turner) C.Agardh Menggunakan KLT-Densitometri

Wahyuningsih,

Lissa Putri Amannillah,

Febriananda

et al. 2024

Camellia

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Pendahuluan: Fukosantin merupakan pigmen alami yang terdapat pada Sargassum ilicifolium (Turner) C.Agardh dan memiliki banyak aktivitas biologis antara lain sebagai antioksidan, imunomodulator, antikanker maupun antidiabetik. Untuk penetapan kadar fukosantin, metode analisis kromatografi lapis tipis-densitometri (KLT-Densitometri) merupakan metode yang selektif dan sederhana untuk diaplikasikan dalam penelitian ini dengan menggunakan marker fukosantin. Tujuan: Menentukan kondisi analisis yang optimum dan penentuan batas deteksi untuk analisis kadar fukosantin dalam Sargassum ilicifolium (Turner) C.Agardh menggunakan KLT-Densitometri. Metode: Penentuan kondisi optimum dilakukan dengan cara penentuan komposisi fase gerak, volume penotolan, waktu penjenuhan bejana dan Panjang gelombang analisis. Penentuan batas deteksi dilakukan dengan menganalisis larutan standar pada 1-40 µg/ml. Hasil: Heksana: aseton (6:4 v/v) dengan panjang gelombang 398 nm, waktu penjenuhan bejana 30 menit dan volume penotolan 8 µl merupakan komposisi fase gerak terpilih karena menghasilkan pemisahan dengan puncak yang simetris dengan batas deteksi 2,5 µg/ml. Kesimpulan: Pada penelitian ini telah diperoleh kondisi optimum dan batas deteksi untuk analisis fukosantin. Kondisi optimum yang diperoleh dapat dilakukan validasi metode analisis. Fukosantin tidak stabil selama penyimpanan sehingga larutan baku dan sampel sebaiknya dibuat baru Ketika dilakukan analisis.

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EKSTRAKSI DAN PURIFIKASI FUKOSANTIN DARI RUMPUT LAUT COKELAT Sargassum sp. SEBAGAI ANTIOKSIDAN

Cited by 4 publications

References 9 publications

Isolation of Fucoxanthin from Sargassum oligocystum Montagne, 1845 Seaweed in Vietnam and Its Neuroprotective Activity

Isolation of Fucoxanthin from Sargassum oligocystum Montagne, 1845 Seaweed in Vietnam and Its Neuroprotective Activity

Elucidation of Antioxidant and Neuroprotective Potential of Fucoxanthin Isolated from Brown Seaweed Sargassum oligocystum

Optimasi Metode dan Penentuan Batas Deteksi untuk Analisis Kadar Fukosantin dalam Sargassum ilicifolium (Turner) C.Agardh Menggunakan KLT-Densitometri

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