Ekstraksi DNA dari Daging Segar untuk Analisis dengan Metode Loop-Mediated Isothermal Amplification (LAMP)

Hutami, Rosy; Bisyri, Hanifah; Sukarno, Sukarno; Nuraini, Henny; Ranasasmita, Raafqi

doi:10.30997/jah.v4i2.1409

Cited by 7 publications

(6 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…If the purity value of the DNA extract is less than 1.8, it indicates that the DNA extract still contains phenol residues and other solvent contaminants. The phenol-chloroform extraction method with a sample weight of 50 mg (Table 2) was able to produce the concentration and purity of DNA extract required for molecular analysis [16]. In the results of DNA amplification (Fig.…”

Section: Resultsmentioning

confidence: 99%

Optimization of DNA Isolation on Meat-Based Food Samples Using Phenol-Chloroform Method for the Development of Halal Detection Using In-House Method Analysis

Mardhotillah,

Achyar,

Chatri

et al. 2023

Advances in Biological Sciences Research

View full text Add to dashboard Cite

About 85% of Indonesia's population is Moslem. Every Moslem is ordered to eat halal and good food. PCR is the most widely used molecular biology technique for authentication tests, through target DNA analysis, including the detection of pork-derived DNA in food products. DNA isolation is an important step that determines the success of PCR, especially for food samples that have gone through various cooking processes at high temperatures, thus affecting the quantity and quality of DNA isolation results. The phenol-chloroform-based DNA isolation method is a method that's considered capable of producing high-quality DNA although the process is slightly longer than using commercial kits. This study aims to obtain optimum DNA isolation results using the phenol-chloroform method in meat-based food samples. DNA quality was tested pork-specific ND5 gene PCR Isolation was carried out on pork and meatball samples which were weighed as much as 50 mg and 100 mg. The results showed DNA isolation with a sample weight of 50 mg was better than a sample weight of 100 mg. The DNA concentration at the sample weight of 50 mg and 100 mg were 423.35 ng/µl and 7472.9 ng/µl. DNA purity at the sample of 50 mg and 100 mg were 1.7 and 1.0.

show abstract

Section: Resultsmentioning

confidence: 99%

Optimization of DNA Isolation on Meat-Based Food Samples Using Phenol-Chloroform Method for the Development of Halal Detection Using In-House Method Analysis

Mardhotillah,

Achyar,

Chatri

et al. 2023

Advances in Biological Sciences Research

View full text Add to dashboard Cite

show abstract

“…The initial step in DNA analysis is DNA extraction. This is useful for separating other cell components such as lipids, proteins, carbohydrates, and other substances [7]. It started with breaking the cell wall with a bead-beating process of the solution by inserting the sample into a 1.5 ml tube and adding GST buffer for centrifugation.…”

Section: Dna Extraction Amplification and Sequencingmentioning

confidence: 99%

Environmental suitability and detection of cnidarian invasive species at Dumai and Kuala Tanjung Port using eDNA

Hartanto,

Lestari,

Sitanala

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

View full text Add to dashboard Cite

The Malacca Strait is Indonesia’s most strategic traffic lane to support ship logistics activities. Ships with ballast water transfer systems can raise water quality issues, distribute foreign microorganisms, or introduce invasive species globally. Phylum Cnidaria has a strong potential to invade waters because all species in this phylum have nematocysts that function to penetrate toxins. This study aimed to determine oceanographic conditions and identify invasive species in the phylum Cnidaria based on eDNA approaches in the waters of the Rupat Strait and Kuala Tanjung Port. Sample identification found 114 species of Cnidaria at Dumai Port and 112 species at Kuala Tanjung Port. Fertility waters were detected to have mesotrophic status at Dumai Port and Eutrophic at Kuala Tanjung Port. Based on the Global invasive species database, we found Ectopleura crocea and Pelagia noctiluca, native to the Mediterranean Sea and North Atlantic Ocean. Environment parameter conditions support the existence of invasive species in both study sites.

show abstract

“…Hasil sentrifugasi berupa 2 fase terpisah yaitu fase fenol dan fase pengotor. Fase fenol yang diambil, tujuan penambahan fenol untuk melarutkan pengotor yang berada didalam ekstrak kasar enzim, maka dari itu protein akan berada pada fase fenol sedangkan zat lain ikut larut dalam fase air [13].…”

Section: Pemurnian Parsial Ekstrak Enzim Daun Pepayaunclassified

Potensi Ekstrak Enzim Daun Pepaya (Carica papaya L.) Sebagai Agen Fibrinolitik Dengan Metode Clot Lysis in Vitro

Warninghiyun,

Indrayati,

RSP

2023

JSSCR

View full text Add to dashboard Cite

Stroke is a disease caused due to blockage of blood vessels. Health department in 2013 stated that 15,4% of people died from strokes. One of the treatment therapies that can be used is fibrinolytic. Fibrinolytic agents have a role in lysing blood clots, these agents can be obtained from microbes, plants and animals. This study aims to determine the protein content contained in papaya leaf enzyme extract (Carica papaya L.), and to determine the potential of the enzyme extract at certain concentrations as an alternative to natural fibrinolytic drugs. The study began with plant determination, sampling, enzyme extraction using a blender, purification of the extract by precipitation using ammonium sulphate, protein content determination using the Lowry method, and testing the fibrinolytic activity of the clot lysis method in vitro. Three series of test concentrations were made, namely 20, 40, and 80%. The results of the research on papaya leaf extract showed that the protein content in purification pellet 1 was 196,22 µg/ml and purification pellet 2 was 306,33 µg/ml. In papaya leaves, fibrinolytic activity was proven with the optimal percentage of blood clot lysis in purification pellet 2 with a concentration of 80% which was 79%.

show abstract

Ekstraksi DNA dari Daging Segar untuk Analisis dengan Metode Loop-Mediated Isothermal Amplification (LAMP)

Cited by 7 publications

References 6 publications

Optimization of DNA Isolation on Meat-Based Food Samples Using Phenol-Chloroform Method for the Development of Halal Detection Using In-House Method Analysis

Optimization of DNA Isolation on Meat-Based Food Samples Using Phenol-Chloroform Method for the Development of Halal Detection Using In-House Method Analysis

Environmental suitability and detection of cnidarian invasive species at Dumai and Kuala Tanjung Port using eDNA

Potensi Ekstrak Enzim Daun Pepaya (Carica papaya L.) Sebagai Agen Fibrinolitik Dengan Metode Clot Lysis in Vitro

Contact Info

Product

Resources

About