El cultivo in vitro de esporas  y los aspectos reproductivos del helecho anual Anogramma chaerophylla (Pteridaceae).

Luna, María Luján; Yañez, Agustina; Giacosa, Juan Pablo Ramos; Giacosa, Ramos; Berrueta, Pedro Cayetano; Giudice, Gabriela Elena

doi:10.31055/1851.2372.v51.n4.16356

Cited by 7 publications

(10 citation statements)

References 11 publications

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“…Pengamatan di bawah mikroskop menunjukkan adanya chlorocyte atau rizoid (Gambar 2) yang muncul dari spora pada hari ke-8 setelah semai. Adanya chlorocyte yang muncul setelah spora disemai, menandakan perkecambahan telah dimulai (Ranal 1999 (Landis et al 2014). Spora yang telah berkecambah selanjutnya memasuki tahap filamen, yaitu sel-sel berwarna hijau terus membelah, dan membentuk seperti benang (Gambar 2).…”

Section: Denticulataunclassified

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MORFOLOGI SPORA DAN PERKEMBANGAN GAMETOFIT Davallia denticulata dan Davallia trichomanoides

Meliza

Chikmawati

Sulistijorini

2019

J Bioteknol Biosains Indones

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Davallia denticulata and D. trichomanoides are two attractive and decorative fern species for ornamental. Spore morphology has an important role in fern taxonomy, while media composition has important role in the growth and development of their gametophytes. Such information on the two fern species was lacking. Therefore, this study aimed to reveal the information of the spore morphology and gametophyte developmental stages of D. denticulata and D. trichomanoides on three different media. The spores were collected from Bogor, West Java. The spores were sown in three sterile media. Spore morphology and gametophyte development were observed under a stereoscopic microscope. Both gametophyte species reached their mature stage at 25 weeks after planting on the different media compositions. D. denticulata showed the best gametophyte development, and formed mature gametophytes on the media of vermiculite, sphagnum moss, and perlite, while D. trichomanoides grew best into maturity stage on the media containing vermiculite, and sphagnum moss. Thus, the presence of sphagnum moss in the media is an important material for the growth and development of Davallia gametophyte. ABSTRAKDavallia denticulata dan D. trichomanoides merupakan dua spesies tumbuhan paku yang menarik dan indah untuk tanaman hias. Morfologi spora memiliki arti penting dalam taksonomi tumbuhan paku, sedangkan komposisi media berperan penting untuk pertumbuhan dan perkembangan gametofitnya. Informasi seputar hal ini terkait dua spesies tumbuhan paku tersebut belumlah ada. Penelitian ini bertujuan untuk mengungkap informasi mengenai ciri morfologi spora dan tahapan perkembangan gametofit D. denticulata dan D. trichomanoides pada tiga komposisi media berbeda. Pengambilan spora dilakukan di Bogor, Jawa Barat. Spora ditumbuhkan pada tiga media steril. Morfologi spora dan perkembangan gametofit diamati menggunakan mikroskop stereo. Kedua spesies memiliki waktu perkembangan terbaik untuk mencapai tahap gametofit dewasa yaitu 25 minggu pada komposisi media yang berbeda. D. denticulata berkembang dengan baik, dan membentuk gametofit dewasa pada media vermiculite, lumut sphagnum, dan perlite. D. trichomanoides berkembang hingga tahap gametofit dewasa dengan baik pada media vermiculite, dan lumut sphagnum. Dengan demikian keberadaan lumut sphagnum pada media sangat penting untuk pertumbuhan dan perkembangan gametofit Davallia.

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Section: Denticulataunclassified

“…Spora D. trichomanoides yang disemai pada tiga jenis media berkecambah dalam waktu yang sama yaitu pada hari ke-6 hingga 8 (Tabel 1) yang ditandai dengan munculnya rhizoid (Gambar 3). Spora dari tumbuhan paku jenis lain, Annogramma chaerophylla (Pteridaceae), juga berkecambah pada hari ke-6 setelah tanam (Luna et al 2016).…”

Section: Trichomanoidesunclassified

MORFOLOGI SPORA DAN PERKEMBANGAN GAMETOFIT Davallia denticulata dan Davallia trichomanoides

Meliza

Chikmawati

Sulistijorini

2019

J Bioteknol Biosains Indones

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“…Since 2006 this research group has been developing a project to explore the diversity and reproductive biology of native ferns in Punta Lara Reserve, based on studies on spore germination and sporophyte development, as a contribution to their conservation (i.e., Giudice et al 2011Giudice et al , 2014Ramos Giacosa et al 2014Luna et al 2016;Gorrer et al 2018). Until the start of this study, there were no other investigations that addressed the soil spore banks in this country.…”

Section: Introductionmentioning

confidence: 99%

Secrets beneath the soil: recovery of fern spores as a strategy of biodiversity conservation in Punta Lara Nature Reserve (PLNR), Argentina

et al. 2021

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The recovery of soil spores is a strategy to strengthen in decline or disappeared populations from natural environments. In this work, we analyzed 25 soil samples extracted from a patch of gallery forest in an “albardón” of Punta Lara Reserve, Buenos Aires. The samples were distributed in 50 Petri dishes, 25 exposed to controlled temperature and light and another 25 kept in darkness. To contribute to the identification of gametophytes and sporophytes, spores of the local species were cultured in vitro. In 18 months of trial, the appearance of gametophytes and sporophytes was observed, in a greater proportion those belonging to a dominant species in the community: Doryopteris concolor. Numerous gametophytes and sporophytes from Gastoniella chaerophylla were also obtained, a taxon not found in the “albardón” for two years. The germination index (GI) was estimated and the morphological characteristics of the gametophytes and sporophytes were recorded. This is the first contribution to the knowledge of the spore banks in Argentina, in a protected area where several threats put at risk the survival of native species. The bases to implement methods of ex situ and in situ conservation of native ferns are provided.

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“…Our studies in this area also included the reproductive aspects of some native ferns, as well as the analyzes of soil spore banks as contributions to biodiversity conservation in this area (Ramos Giacosa et al, 2014Luna et al, 2016;Ramos Giacosa et al, 2017;Gorrer et al, 2018).…”

mentioning

confidence: 99%

Atlas de esporas de helechos isosporados de la Reserva Natural Punta Lara, Argentina

Gorrer

Berrueta

Giacosa

et al. 2021

Bol. Soc. Argent. Bot.

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Introducción y objetivo: Las características morfológicas de las esporas brindan información para la identificación de taxa de helechos. Profundizar los análisis de las características morfológicas de las esporas de los helechos isosporados que habitan en la Reserva Natural de Punta Lara utilizando microscopía óptica. M&M: Se estudiaron con microscopio óptico las esporas de helechos isosporados que crecen en La Reserva Natural Punta Lara (provincia de Buenos Aires, Argentina). El estudio fue llevado a cabo con material fresco colectado durante 3 años (2016-2018) en diferentes sitios de la Reserva. Se analizaron 20 taxa de helechos, 16 nativos y 4 exóticos. Las características registradas fueron: color, forma, diámetros ecuatoriales mayor y menor, diámetro polar, longitud y características de la lesura y ornamentación de la pared. Resultados: El color de las esporas puede ser amarillo, castaño claro, castaño oscuro y solo en el caso de Equisetum son verdes (clorofílicas). La mayoría de esporas son monoletes (13 especies), otras triletes (6 especies) y una sola especie posee esporas aletes. Las paredes de las esporas pueden ser lisas, plegadas, verrucosas, tuberculadas, reticuladas y ruguladas-crestadas. Conclusiones: La morfología de las esporas permite la identificación de los helechos de la Reserva a nivel de género y especie y aporta información para estudios paleopalinológicos, aeropalinológicos y de bancos de esporas del suelo.

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El cultivo in vitro de esporas y los aspectos reproductivos del helecho anual Anogramma chaerophylla (Pteridaceae).

Cited by 7 publications

References 11 publications

MORFOLOGI SPORA DAN PERKEMBANGAN GAMETOFIT <em>Davallia denticulata</em> dan <em>Davallia trichomanoides</em>

MORFOLOGI SPORA DAN PERKEMBANGAN GAMETOFIT <em>Davallia denticulata</em> dan <em>Davallia trichomanoides</em>

Secrets beneath the soil: recovery of fern spores as a strategy of biodiversity conservation in Punta Lara Nature Reserve (PLNR), Argentina

Atlas de esporas de helechos isosporados de la Reserva Natural Punta Lara, Argentina

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