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Tenorio, Germán; Orea, Alicia; Romero, Javier; Mérida, Ángel

doi:10.1023/a:1023053420632

Cited by 66 publications

(22 citation statements)

References 27 publications

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“…Tenioro et al . [43] showed that GBSS is strongly regulated by the circadian clock and that expression is markedly lower in mutants lacking clock genes LHY and CCA-OX respectively. In a detailed study done on GBSS in snapdragon ( Antirrhinum majus ) it was shown that GBSS is diurnally regulated in leaves even under continuous light and it was concluded that the regulation is due to the circadian clock.…”

Section: Resultsmentioning

confidence: 99%

Comparative transcriptome analysis coupled to X-ray CT reveals sucrose supply and growth velocity as major determinants of potato tuber starch biosynthesis

et al. 2010

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BackgroundEven though the process of potato tuber starch biosynthesis is well understood, mechanisms regulating biosynthesis are still unclear. Transcriptome analysis provides valuable information as to how genes are regulated. Therefore, this work aimed at investigating transcriptional regulation of starch biosynthetic genes in leaves and tubers of potato plants under various conditions. More specifically we looked at gene expression diurnally in leaves and tubers, during tuber induction and in tubers growing at different velocities. To determine velocity of potato tuber growth a new method based on X-ray Computed Tomography (X-ray CT) was established.ResultsComparative transcriptome analysis between leaves and tubers revealed striking similarities with the same genes being differentially expressed in both tissues. In tubers, oscillation of granule bound starch synthase (GBSS) expression) was observed which could be linked to sucrose supply from source leaves. X-ray CT was used to determine time-dependent changes in tuber volume and the growth velocity was calculated. Although there is not a linear correlation between growth velocity and expression of starch biosynthetic genes, there are significant differences between growing and non-growing tubers. Co-expression analysis was used to identify transcription factors positively correlating with starch biosynthetic genes possibly regulating starch biosynthesis.ConclusionMost starch biosynthetic enzymes are encoded by gene families. Co-expression analysis revealed that the same members of these gene families are co-regulated in leaves and tubers. This suggests that regulation of transitory and storage starch biosynthesis in leaves and tubers, respectively, is surprisingly similar. X-ray CT can be used to monitor growth and development of belowground organs and allows to link tuber growth to changes in gene expression. Comparative transcriptome analysis provides a useful tool to identify transcription factors possibly involved in the regulation of starch biosynthesis.

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Section: Resultsmentioning

confidence: 99%

Comparative transcriptome analysis coupled to X-ray CT reveals sucrose supply and growth velocity as major determinants of potato tuber starch biosynthesis

et al. 2010

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“…The GBSS activity was assayed using the method previously described in (Tenorio et al 2003). Starch granules were washed three times in 100 mM Tris buffer, pH 7.2, 2 mM EDTA and 1 mM DTT.…”

Section: Granule-bound Starch Synthase Assaymentioning

confidence: 99%

Two distinct waxy alleles impact the granule-bound starch synthase in sorghum

et al. 2009

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The granule-bound starch synthase (GBSS) is the enzyme responsible for amylose synthesis in starch granules. Loss of GBSS activity results in starch granules containing mostly amylopectin and little or no amylose, a phenotype described as waxy. Previously, two phenotypic classes of waxy alleles were identified in sorghum (Sorghum bicolor L. Moench) characterized by the absence (waxy a ; wx a ) or presence (waxy b ; wx b ) of the GBSS protein in the endosperm. To characterize these alleles, we examined endosperm architecture using scanning electron microscopy (SEM), assayed GBSS enzymatic activities, and identified DNA lesions associated with the mutations in the GBSS (Sb10g002140) gene. wx a , the allele present in B Tx630 and R Tx2907, contained a large insertion in the third exon, which was consistent with the absence of the GBSS protein previously observed. wx b , the allele present in B 9307 and B TxARG1, contained a missense mutation that resulted in conversion of glutamine 268 to histidine in a conserved domain in starch synthases. In wx b , GBSS activity was less than 25% that of the non-waxy line B Wheatland, and GBSS activity was not detected in wx a . SEM showed that endosperm architecture was very similar in both wx a and wx b alleles, but altered in comparison to non-waxy lines R Tx430 and B Wheatland. Both alleles may have a range of potential applications in grain sorghum because of low amylose content in their starch and the presence or absence of the GBSS protein. PCR based markers were developed for both the wx a and the wx b alleles to aid in molecular breeding of low amylose sorghum.

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“…Transcript abundance of many starch genes is regulated by the circadian clock and by sugar availability [8, 10, 11]. In Arabidopsis leaves, expression of the GBSS1 gene is controlled by two clock transcription factors (TFs), namely the Myb-related CIRCADIAN CLOCK ASSOCIATED 1 (CCA1) and LATE ELONGATED HYPOCOTYL (LHY) [12], while in rice endosperm, GBSS was reported to be regulated by two interacting proteins belonging to the MYC and EREBP families [13]. Further evidence for transcriptional regulation of starch metabolism comes from barley, where a sugar-inducible TF, SUSIBA2, belonging to the WRKY class of TFs, bound to the promoter of the ISA1 gene and exhibited an expression pattern similar to ISA1 [14].…”

Section: Introductionmentioning

confidence: 99%

Genome-wide analysis of starch metabolism genes in potato (Solanum tuberosum L.)

et al. 2017

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BackgroundStarch is the principle constituent of potato tubers and is of considerable importance for food and non-food applications. Its metabolism has been subject of extensive research over the past decades. Despite its importance, a description of the complete inventory of genes involved in starch metabolism and their genome organization in potato plants is still missing. Moreover, mechanisms regulating the expression of starch genes in leaves and tubers remain elusive with regard to differences between transitory and storage starch metabolism, respectively. This study aimed at identifying and mapping the complete set of potato starch genes, and to study their expression pattern in leaves and tubers using different sets of transcriptome data. Moreover, we wanted to uncover transcription factors co-regulated with starch accumulation in tubers in order to get insight into the regulation of starch metabolism.ResultsWe identified 77 genomic loci encoding enzymes involved in starch metabolism. Novel isoforms of many enzymes were found. Their analysis will help to elucidate mechanisms of starch biosynthesis and degradation. Expression analysis of starch genes led to the identification of tissue-specific isoenzymes suggesting differences in the transcriptional regulation of starch metabolism between potato leaf and tuber tissues. Selection of genes predominantly expressed in developing potato tubers and exhibiting an expression pattern indicative for a role in starch biosynthesis enabled the identification of possible transcriptional regulators of tuber starch biosynthesis by co-expression analysis.ConclusionsThis study provides the annotation of the complete set of starch metabolic genes in potato plants and their genomic localizations. Novel, so far undescribed, enzyme isoforms were revealed. Comparative transcriptome analysis enabled the identification of tuber- and leaf-specific isoforms of starch genes. This finding suggests distinct regulatory mechanisms in transitory and storage starch metabolism. Putative regulatory proteins of starch biosynthesis in potato tubers have been identified by co-expression and their expression was verified by quantitative RT-PCR.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-016-3381-z) contains supplementary material, which is available to authorized users.

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Cited by 66 publications

References 27 publications

Comparative transcriptome analysis coupled to X-ray CT reveals sucrose supply and growth velocity as major determinants of potato tuber starch biosynthesis

Comparative transcriptome analysis coupled to X-ray CT reveals sucrose supply and growth velocity as major determinants of potato tuber starch biosynthesis

Two distinct waxy alleles impact the granule-bound starch synthase in sorghum

Genome-wide analysis of starch metabolism genes in potato (Solanum tuberosum L.)

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