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Bang, S. S.; Clark, Douglas S.; Keasling, Jay D.

doi:10.1023/a:1005611331948

Cited by 23 publications

(2 citation statements)

References 19 publications

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“…In fact, E. coli is intrinsically tolerant to high levels of cadmium up to 0.9-1.0 mM. 16,25,26 However the exact mechanism of cadmium resistance in E. coli is not fully understood. To date, we know that ZntA, a Pb(II)-, Zn(II)-, and Cd(II)-transporting ATPases confer cadmium tolerance in E. coli.…”

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confidence: 99%

Identification of cadmium resistance and adsorption gene from Escherichia coli BL21 (DE3)

Qin

Liu

et al. 2017

RSC Adv.

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Cadmium is recognized as one of the most toxic heavy metals, and chronic cadmium exposure threatens plant, animal and human health. Because certain bacteria can play an important role in remedying heavy metal pollution, it is essential to further our understanding and clone cadmium adsorption and resistance genes from microorganisms. In this study, the cadmium resistance of different Escherichia coli strains including BL21 (DE3), JM109, DH5a, Top10 and Mach was determined. Among those strains, the E. coli BL21 (DE3) exhibited the highest cadmium resistance. Sequence analyses of four cadmium resistant clones screened from the BL21 (DE3) fosmid library showed that the insertion of each fosmid plasmid contained a fragment located at the lambda phage DE3 region. Next, we overexpressed several genes of this fragment in BL21 (DE3) and identified that cadmium resistance of E. coli is acquired through the capB gene. Indeed, overexpression of the capB gene increased the adsorption rate of cadmium. Taken together, these results indicated that the capB gene confers E. coli BL21 (DE3) with increased cadmium resistance by adsorption of cadmium. This study provided new insight into the mechanism of microbial cadmium resistance and expanded our understanding of cadmium resistance, adsorption and bioremediation by microorganisms.

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Section: Introductionmentioning

confidence: 99%

Identification of cadmium resistance and adsorption gene from Escherichia coli BL21 (DE3)

Qin

Liu

et al. 2017

RSC Adv.

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“…Lead has been found in at least 1026 of 1467 National Priorities List sites identified by the US Environmental Protection Agency [5]. Lead is used as an industrial raw material for storage battery manufacturing, printing, pigments, fuels, photographic materials and explosive manufacturing [6]. Perhaps no other metal, not even arsenic, has had its toxicology as extensively studied as has lead [7].…”

Section: Introductionmentioning

confidence: 99%

Investigation on <i>Pleurotus ferulae</i> potential for the sorption of Pb(II) from aqueous solution

Adebayo¹

2012

Bull. Chem. Soc. Eth.

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ABSTRACT. Pleurotus ferulae obtained from rotten tree was collected, washed, dried, ground and sieved to appropriate particle size. Infra-red spectrometry was used to determine functional groups on the biomass while biosorption of Pb(II) from aqueous solution was studied using the biomass in a batch system. The effect of pH (1-7.5), temperature (30-50 o C), biomass dosage of 0.5-4 g and initial metal concentration (20-300 mg/L) was studied. Equilibrium, kinetics and thermodynamics consideration were evaluated from the studied parameters to evaluate the adsorption potential of the biomass. The functional groups on the dried P. ferulae were: -OH, -NH, -COO − and -C-O. In the batch experiments maximum biosorption was observed at initial pH of 3.0, temperature of 30 o C and at the initial metal concentration of 100 mg/L. A contact time 20 min was sufficient for quantitative sorption of lead. It was observed that pseudo second order rate expression fit the experimental data and equilibrium analysis showed that the biosorption process followed Langmuir model. Thermodynamic analysis showed that the process was exothermic and ∆G o was negative showing spontaneity of the process within the studied temperature.

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Cadmium as an Environmental Contaminant: Consequences to Plant and Human Health

Kuriakose

Prasad

2008

Trace Elements as Contaminants and Nutrients

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Cited by 23 publications

References 19 publications

Identification of cadmium resistance and adsorption gene from Escherichia coli BL21 (DE3)

Identification of cadmium resistance and adsorption gene from Escherichia coli BL21 (DE3)

Investigation on <i>Pleurotus ferulae</i> potential for the sorption of Pb(II) from aqueous solution

Cadmium as an Environmental Contaminant: Consequences to Plant and Human Health

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