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Igartua, Ernesto; Gracia, María Pilar Rivero; Lasa, J. M.; Médina, B.; Molina-Cano, José Luis; Montoya, José Luis; Romagosa, I.

doi:10.1023/a:1008662515059

Cited by 63 publications

(25 citation statements)

References 10 publications

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“…We investigated the distribution of the PPDH2 alleles over a sample of 162 barley cultivars of different geographic origins (Additional file 2) and 159 Spanish landrace-derived inbred lines from the Spanish Barley Core Collection (SBCC) [33]. Lines were classified according to their seasonal growth habit, on the basis of the allelic constitution at VRNH1 and VRNH2 (Table 1).…”

Section: Resultsmentioning

confidence: 99%

“…The genotypes consisted of the French cultivars Rebelle ((Barbarrosa × Monarca) × Pirate), Plaisant (Ager × Nymphe), Hispanic (Mosar × (Flika × Lada)), and Arlois (unknown pedigree), and four inbred lines, derived from landraces, that belong to the SBCC [33]. The genotypes studied have different VRNH1 - VRNH3 allelic combinations and all can be classified as 'winter' genotypes.…”

Section: Methodsmentioning

confidence: 99%

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Adaptation of barley to mild winters: A role for PPDH2

et al. 2011

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BackgroundUnderstanding the adaptation of cereals to environmental conditions is one of the key areas in which plant science can contribute to tackling challenges presented by climate change. Temperature and day length are the main environmental regulators of flowering and drivers of adaptation in temperate cereals. The major genes that control flowering time in barley in response to environmental cues are VRNH1, VRNH2, VRNH3, PPDH1, and PPDH2 (candidate gene HvFT3). These genes from the vernalization and photoperiod pathways show complex interactions to promote flowering that are still not understood fully. In particular, PPDH2 function is assumed to be limited to the ability of a short photoperiod to promote flowering. Evidence from the fields of biodiversity, ecogeography, agronomy, and molecular genetics was combined to obtain a more complete overview of the potential role of PPDH2 in environmental adaptation in barley.ResultsThe dominant PPDH2 allele is represented widely in spring barley cultivars but is found only occasionally in modern winter cultivars that have strong vernalization requirements. However, old landraces from the Iberian Peninsula, which also have a vernalization requirement, possess this allele at a much higher frequency than modern winter barley cultivars. Under field conditions in which the vernalization requirement of winter cultivars is not satisfied, the dominant PPDH2 allele promotes flowering, even under increasing photoperiods above 12 h. This hypothesis was supported by expression analysis of vernalization-responsive genotypes. When the dominant allele of PPDH2 was expressed, this was associated with enhanced levels of VRNH1 and VRNH3 expression. Expression of these two genes is needed for the induction of flowering. Therefore, both in the field and under controlled conditions, PPDH2 has an effect of promotion of flowering.ConclusionsThe dominant, ancestral, allele of PPDH2 is prevalent in southern European barley germplasm. The presence of the dominant allele is associated with early expression of VRNH1 and early flowering. We propose that PPDH2 promotes flowering of winter cultivars under all non-inductive conditions, i.e. under short days or long days in plants that have not satisfied their vernalization requirement. This mechanism is indicated to be a component of an adaptation syndrome of barley to Mediterranean conditions.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Adaptation of barley to mild winters: A role for PPDH2

et al. 2011

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“…In this paper, we analyzed the extent of HvFT1 CNV and its effect on HvFT1 expression in more than 100 different spring and winter barley genotypes, mainly European, including some landraces from the Spanish Barley Core Collection (SBCC) (Igartua et al, 1998). Recently, Nitcher et al (2013) demonstrated that the FT1 allele present in the barley genetic stock BGS213, which carried several copies of HvFT1 , showed earlier transcriptional up-regulation of FT1 , and was associated with a dominant spring growth habit.…”

Section: Discussionmentioning

confidence: 99%

“…A set of 109 genotypes was used to survey the polymorphisms present at HvFT1 , 89 cultivars, mainly European, and 20 inbred lines derived from Spanish landraces (Igartua et al, 1998). They were classified into 60 winter and 49 spring types, according to their genetic constitution at vernalization and photoperiod genes (Table S1).…”

Section: Methodsmentioning

confidence: 99%

HvFT1 polymorphism and effectâ€”survey of barley germplasm and expression analysis

Loscos

Igartua²,

Contreras‐Moreira³

et al. 2014

Front. Plant Sci.

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Flowering time in plants is a tightly regulated process. In barley (Hordeum vulgare L.), HvFT1, ortholog of FLOWERING LOCUS T, is the main integrator of the photoperiod and vernalization signals leading to the transition from vegetative to reproductive state of the plant. This gene presents sequence polymorphisms affecting flowering time in the first intron and in the promoter. Recently, copy number variation (CNV) has been described for this gene. An allele with more than one copy was linked to higher gene expression, earlier flowering, and an overriding effect of the vernalization mechanism. This study aims at (1) surveying the distribution of HvFT1 polymorphisms across barley germplasm and (2) assessing gene expression and phenotypic effects of HvFT1 alleles. We analyzed HvFT1 CNV in 109 winter, spring, and facultative barley lines. There was more than one copy of the gene (2–5) only in spring or facultative barleys without a functional vernalization VrnH2 allele. CNV was investigated in several regions inside and around HvFT1. Two models of the gene were found: one with the same number of promoters and transcribed regions, and another with one promoter and variable number of transcribed regions. This last model was found in Nordic barleys only. Analysis of HvFT1 expression showed that association between known polymorphisms at the HvFT1 locus and the expression of the gene was highly dependent on the genetic background. Under long day conditions the earliest flowering lines carried a sensitive PpdH1 allele. Among spring cultivars with different number of copies, no clear relation was found between CNV, gene expression and flowering time. This was confirmed in a set of doubled haploid lines of a population segregating for HvFT1 CNV. Earlier flowering in the presence of several copies of HvFT1 was only seen in cultivar Tammi, which carries one promoter, suggesting a relation of gene structure with its regulation. HvCEN also affected to a large extent flowering time.

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“…For analyzing drought stress, a set of barley genotypes [45,[67][68][69] was used consisting of 113 German winter barley cultivars and 43 accessions of the Spanish Barley Core Collection (SBCC). The materials were listed in detail in Additional file 1 in Wehner, et al [45].…”

Section: Methodsmentioning

confidence: 99%

QTL for Water Use Related Traits in Juvenile Barley

2016

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Water use efficiency (WUE) is a trait of prime interest in cases of drought stress because it provides information on biomass production in limited water conditions. In order to get information on WUE and additional water use related traits, i.e., dry weight (DW), fresh weight (FW), total leaf water (LW) and leaf water content (WC), greenhouse pot experiments were conducted on 156 barley genotypes (Hordeum vulgare L.) for control (70% maximal water capacity of soil) and drought stress conditions (20% of the maximal water capacity of soil). Significant correlations between WUE and the other water use related traits (r ≤ 0.65) were determined in juvenile barley, and genotypes suited for improving drought stress tolerance in early developmental stages were identified. Furthermore, based on the significant effects of genotypes and treatments, as well as their interaction, data were used for genome wide association studies (GWAS) resulting in the identification of 14 marker trait associations (MTAs) corresponding to four quantitative trait loci (QTL). For WUE, four MTAs were detected mostly located on barley chromosome 4H. For four MTAs, functional annotations related to the involvement in response to abiotic stress were found. These markers may be of special interest for breeding purposes in cases when they will be validated and also detected in later growth stages.

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Cited by 63 publications

References 10 publications

Adaptation of barley to mild winters: A role for PPDH2

Adaptation of barley to mild winters: A role for PPDH2

HvFT1 polymorphism and effectâ€”survey of barley germplasm and expression analysis

QTL for Water Use Related Traits in Juvenile Barley

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