Elaboration and Characterization of Coaxial Electrospun Poly(ε‐Caprolactone)/Gelatin Nanofibers for Biomedical Applications

Pereira, Ildeu H.L.; Ayres, Eliane; Avérous, Luc; Schlatter, Guy; Hébraud, Anne; Mendes, S.; Oréfice, Rodrigo Lambert

doi:10.1002/adv.21475

Cited by 12 publications

(7 citation statements)

References 31 publications

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“…Next, it decreased after getting a maximum at an intermediate voltage around 15 kV (1.51 µm average diameter at 13 kV, 1.76 µm at 15 kV and 1.62 µm at 17 kV). This non-monotonic effect of the voltage on the fiber diameter has previously been observed for polymers of a different nature [ 33 , 34 , 35 ]. It was also observed that the higher the voltage, the higher the variability in fiber diameter (standard deviation: 0.14 µm at 13 kV, 0.20 µm at 15 kV and 0.26 µm at 17 kV).…”

Section: Resultssupporting

confidence: 66%

Role of Electrospinning Parameters on Poly(Lactic-co-Glycolic Acid) and Poly(Caprolactone-co-Glycolic acid) Membranes

2021

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Poly(lactic-co-glycolic acid) (PLGA) and poly(caprolactone-co-glycolic acid) (PCLGA) solutions were electrospun into membranes with tailored fiber diameter of 1.8 μm. This particular fiber diameter was tuned depending on the used co-polymer by adjusting the electrospinning parameters that mainly influence the fiber diameter. The greatest setting of the fiber diameter was achieved by varying the polymer solution parameters (polymer concentration, solvents and solvents ratio). PLGA was adequately electrospun with 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP), whereas PCLGA required a polar solvent (such as chloroform) with a lower dielectric constant. Moreover, due to the amorphous morphology of PCLGA, pyridine as salt had to be added to the starting solution to increase its conductivity and make it electrospinnable. Indeed, the electrospinning of this co-polymer presents notable difficulties due to its amorphous structure. Interestingly, PCLGA, having a higher glycolic acid molar fraction than commonly electrospun co-polymers (caprolactone:glycolic acid ratio of 45:55 instead of 90:10), could be successfully electrospun, which has not been reported to date. To an accurate setting of fiber diameter, the voltage and the distance from needle to collector were varied. Finally, the study of the surface tension, conductivity and viscosity of the polymer solutions allowed to correlate these particular characteristics of the solutions with the electrospinning variables so that prior knowledge of them enables predicting the required processing conditions.

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Section: Resultssupporting

confidence: 66%

Role of Electrospinning Parameters on Poly(Lactic-co-Glycolic Acid) and Poly(Caprolactone-co-Glycolic acid) Membranes

2021

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“…As expected, the typical absorption bands of gelatin were detected in ATR-FTIR spectra of all samples, i.e. the characteristic peak at: 1632 cm -1 , related to the stretching vibration of C=O bond (amide I); 1540 cm -1 related to the C-N stretching vibrations (amide II) and N-H bending vibrations; 1238 cm -1 describing the CH2 wagging vibrations (amide III); and at 3288 cm -1 regarding the N-H stretching vibration [92][93][94][95][96]. Other than gelatin-related bands, characteristic absorption bands of the PCL phase could also be identified in electrospun and UV-cured samples at 2949 cm -1 and 2865 cm -1 (regarding the asymmetric CH2 stretching and symmetric CH2 stretching, respectively), at 1727 cm -1 (describing the carbonyl stretching C=O associated with the ester bonds), at 1226 cm 4= (related to the C-O and C-C stretching) and at 1185 cm -1 (regarding the asymmetric COC stretching) [97][98][99].…”

Section: Elucidation Of Chemical Compositionmentioning

confidence: 99%

A UV-cured nanofibrous membrane of vinylbenzylated gelatin-poly(ɛ-caprolactone) dimethacrylate co-network by scalable free surface electrospinning

Bazbouz

Tronci

2018

Materials Science and Engineering: C

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Electrospun nanofibrous membranes of natural polymers, such as gelatin, are fundamental in the design of regenerative devices. Crosslinking of electrospun fibres from gelatin is required to prevent dissolution in water, to retain the original nanofibre morphology after immersion in water, and to improve the thermal and mechanical properties, although this is still challenging to accomplish in a controlled fashion. In this study, we have investigated the scalable manufacture and structural stability in aqueous environment of a UV-cured nanofibrous membrane fabricated by free surface electrospinning (FSES) of aqueous solutions containing vinylbenzylated gelatin and poly(ɛ-caprolactone) dimethacrylate (PCL-DMA). Vinylbenzylated gelatin was obtained via chemical functionalisation with photopolymerisable 4-vinylbenzyl chloride (4VBC) groups, so that the gelatin and PCL phase in electrospun fibres were integrated in a covalent UV-cured co-network at the molecular scale, rather than being simply physically mixed. Aqueous solutions of acetic acid (90 vol%) were employed at room temperature to dissolve gelatin-4VBC (G-4VBC) and PCL-DMA with two molar ratios between 4VBC and DMA functions, whilst viscosity, surface tension and electrical conductivity of resulting electrospinning solutions were characterised. Following successful FSES, electrospun nanofibrous samples were UV-cured using Irgacure I2959 as radical photo-initiator and 1-Heptanol as water-immiscible photo-initiator carrier, resulting in the formation of a water-insoluble, gelatin/PCL covalent co-network. Scanning electron microscopy (SEM), attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy, differential scanning calorimetry (DSC), tensile test, as well as liquid contact angle and swelling measurements were carried out to explore the surface morphology, chemical composition, thermal and mechanical properties, wettability and water holding capacity of the nanofibrous membranes, respectively. UV-cured nanofibrous membranes did not dissolve in water and showed enhanced thermal and mechanical properties, with respect to as-spun samples, indicating the effectiveness of the photo-crosslinking reaction. In addition, UV-cured gelatin/PCL membranes displayed increased structural stability in water with respect to PCL-free samples and were highly tolerated by G292 osteosarcoma cells. These results therefore support the use of PCL-DMA as hydrophobic, biodegradable crosslinker and provide new insight on the scalable design of water-insoluble, mechanical-competent gelatin membranes for healthcare applications.

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“…For the electrospinning of Ge, lowtoxic alternative solvents have also been considered, including acetic acid [53], [54] or ethylacetate/acetic acid in water [55]. Specifically, the formic/acetic mixture has been considered as a suitable candidate for the electrospinning of PCL/Ge scaffolds [56]- [59].…”

Section: Introductionmentioning

confidence: 99%

Tailored electrospun nanofibrous polycaprolactone/gelatin scaffolds into an acid hydrolytic solvent system

Cháfer

Badía

Ribes‐Greus

2018

European Polymer Journal

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Blended nanofibrous scaffolds based on polycaprolactone (PCL) and gelatin (Ge) were successfully prepared. A formic/acetic acid (1:1) mixture was used to dissolve PCL/Ge blends from 100/0 to 20/80 %wt in steps of 10 %wt. The hydrolysis of the PCL diluted in the formic/acetic acid mixture was considered as a method for tailoring the surface morphology and physicochemical features of the nanofibrous PCL/Ge scaffolds as a function of the dissolution time. The fibre diameter remained in the nanoscale range for all the studied scaffolds, which is crucial to mimic the extra-cellular matrix size. The reduction of the intrinsic viscosity, molar mass and hydrodynamic radius found for the PCL molecules as a function of the dissolution time, consequently diminished the entanglement capability of the polymeric chains. Subsequently, the fibre diameter decreased as dissolution time increased, for all the studied compositions. While the crystallinity of the scaffolds with high PCL content increased as a function of the dissolution time, the scaffolds with high percentage of Ge showed the lowest crystallinity degree, which was ascribed to the hindering effect of the Ge diffused among the PCL segments. The wettability increased as a function of the Ge content due to the high hydrophilic behaviour of these molecules. It also increased as a function of the dissolution time, due to the more hydroxyl groups available in PCL segments to interact with water molecules. As a whole, the physicochemical assessment of the electrospun scaffolds revealed an effective tailoring procedure to obtain functionalised PCL/Ge scaffolds with specific properties as a function of the dissolution time before electrospinning.

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Elaboration and Characterization of Coaxial Electrospun Poly(ε‐Caprolactone)/Gelatin Nanofibers for Biomedical Applications

Cited by 12 publications

References 31 publications

Role of Electrospinning Parameters on Poly(Lactic-co-Glycolic Acid) and Poly(Caprolactone-co-Glycolic acid) Membranes

Role of Electrospinning Parameters on Poly(Lactic-co-Glycolic Acid) and Poly(Caprolactone-co-Glycolic acid) Membranes

A UV-cured nanofibrous membrane of vinylbenzylated gelatin-poly(ɛ-caprolactone) dimethacrylate co-network by scalable free surface electrospinning

Tailored electrospun nanofibrous polycaprolactone/gelatin scaffolds into an acid hydrolytic solvent system

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