Elastin‐Like Polymers: Properties, Synthesis, and Applications

Rodríguez‐Cabello, José Carlos; Ibáñez‐Fonseca, Arturo; Alonso, Matilde; Poocza, Leander; Cipriani, Filippo; Torre, Israel González de

doi:10.1002/0471440264.pst656

Cited by 6 publications

(10 citation statements)

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“…Among these biomaterials, elastin-like polypeptides (ELPs) or elastin-like recombinamers (ELRs), depending on whether they are synthesized (Rodriguez-Cabello et al, 2017 ) by traditional chemical synthesis or by recombinant techniques, respectively, have been shown to be promising biocompatible candidates for use in tissue-engineering applications (Ibáñez-Fonseca et al, 2018 ). ELRs are based on the repetition of the five amino acids that confer the elastic properties to the elastin molecule, namely VPGVG, in which the V at the fourth position can be substituted by any amino acid except proline (Rodríguez-Cabello et al, 2018a ).…”

Section: Introductionmentioning

confidence: 99%

Elastin-Based Materials: Promising Candidates for Cardiac Tissue Regeneration

Torre

Alonso

Rodríguez-Cabello

2020

Front. Bioeng. Biotechnol.

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Stroke and cardiovascular episodes are still some of the most common diseases worldwide, causing millions of deaths and costing billions of Euros to healthcare systems. The use of new biomaterials with enhanced biological and physical properties has opened the door to new approaches in cardiovascular applications. Elastin-based materials are biomaterials with some of the most promising properties. Indeed, these biomaterials have started to yield good results in cardiovascular and angiogenesis applications. In this review, we explore the latest trends in elastin-derived materials for cardiac regeneration and the different possibilities that are being explored by researchers to regenerate an infarcted muscle and restore its normal function. Elastin-based materials can be processed in different manners to create injectable systems or hydrogel scaffolds that can be applied by simple injection or as patches to cover the damaged area and regenerate it. Such materials have been applied to directly regenerate the damaged cardiac muscle and to create complex structures, such as heart valves or new bio-stents that could help to restore the normal function of the heart or to minimize damage after a stroke. We will discuss the possibilities that elastin-based materials offer in cardiac tissue engineering, either alone or in combination with other biomaterials, in order to illustrate the wide range of options that are being explored. Moreover, although tremendous advances have been achieved with such elastin-based materials, there is still room for new approaches that could trigger advances in cardiac tissue regeneration.

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Section: Introductionmentioning

confidence: 99%

Elastin-Based Materials: Promising Candidates for Cardiac Tissue Regeneration

Torre

Alonso

Rodríguez-Cabello

2020

Front. Bioeng. Biotechnol.

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“…La capacidad de respuesta térmica es la característica más investigada y mejor controlada para los copolímeros de bloque 27,34 y, como tal, ha ganado un mayor interés en el diseño de proteínas recombinantes de vanguardia, especialmente a la luz de los hallazgos de Urry con respecto a la LCST de péptidos miméticos de elastina [44][45][46][47] . Además, debido a que los ELRs permiten distintos diseños modulares combinando bloque análogos con dominios de diferente hidrofilicidad, se han estudiado como modelos para comprender el plegamiento, el autoensamblaje y la función de otras proteínas naturales más complejas [48][49][50] .…”

Section: Metodología De La Modificación De Elrs Con Grupos Hidrofóbicunclassified

“…Additionally, properties such as stimuli-responsiveness towards changes in temperature 27,34 or pH 35 have resulted in the applicability of these materials in tissue engineering 36 , drug-delivery systems [35][36][37][38] , polymer dispersants 39 , physical gelling agents 40 , or biosensors [41][42][43] . Thermoresponsiveness is the most investigated and best controlled feature for block-copolymers 27,34 and, as such, has gained increased interest in cuttingedge recombinant protein design, especially in light of the findings by Urry regarding the LCST of elastin-mimetic peptides [44][45][46][47] . Furthermore, elastinlike recombinamers/polypeptides (ELR/ELPs), which are block-copolymer analogs with domains of differing hydrophilicity, have been explored as models for understanding the folding, self-assembly, and function of natural proteins 46,47 .…”

Section: Elr In Gel Zymographymentioning

confidence: 99%

“…Thermoresponsiveness is the most investigated and best controlled feature for block-copolymers 27,34 and, as such, has gained increased interest in cuttingedge recombinant protein design, especially in light of the findings by Urry regarding the LCST of elastin-mimetic peptides [44][45][46][47] . Furthermore, elastinlike recombinamers/polypeptides (ELR/ELPs), which are block-copolymer analogs with domains of differing hydrophilicity, have been explored as models for understanding the folding, self-assembly, and function of natural proteins 46,47 . The variety of protein-based features that can be added to ELRs makes them an ideal model for relating properties to structure.…”

Section: Elr In Gel Zymographymentioning

confidence: 99%

“…β-turns are the most widely studied structural feature of ELRs found in the literature as, in an ideal arrangement, they can form concatenated ordered β-helices that could explain the elastic properties of such materials and their thermomechanical behaviour 44,46,112,157,429 . Despite this, a quantitative estimation of protein secondary structures in IDPs remains challenging due to the huge morphological and spectral diversity of structures and their overlap in the CD spectra.…”

Section: Characterization In Solutionmentioning

confidence: 99%

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Accessing new biomedical applications by combining genetic design and chemical modification of elastin-like recombinamers

Poocza¹

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The diversity of nature including biopolymers, organisms, or active molecules and the fact that these natural systems can be transformed to be used for other purposes, enables a myriad of promising starting points for new developments. Here, Elastin-like recombinamers (ELRs) as a class of Abstract groups to foster electrostatic interactions with the negatively charged membrane. Herein we present an antagonistic approach based on ELRs with varying amounts of hydrophobic cholesteryl groups (ELR CTA s). The ability of membranes to stabilize cholesteryl groups is hypothesized to assist the coordination of hydrophobic ELRs with the membrane. The main objective was to generate a defined cellular coating of a recombinant protein that allows for total sequence control and less host, or batch-to-batch, variation as a substitute for existing coatings like alginate, polyelectrolytes, collagens and fibronectin. We used an in vitro cell-binding assay to quantify cellmembrane interactions, with the substrates showing enhanced cellular recognition and matrix distribution with an increasing number of cholesteryl groups incorporated. These novel materials and the versatile nature of their protein sequence have great potential as cellular markers, drug carriers, or hydrophobic cell-binding domains.

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Genetically Engineered Viral Vectors and Organic‐Based Non‐Viral Nanocarriers for Drug Delivery Applications

Hajebi

Yousefiasl

Rahimmanesh

et al. 2022

Adv Healthcare Materials

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Conventional drug delivery systems are challenged by concerns related to systemic toxicity, repetitive doses, drug concentrations fluctuation, and adverse effects. Various drug delivery systems are developed to overcome these limitations. Nanomaterials are employed in a variety of biomedical applications such as therapeutics delivery, cancer therapy, and tissue engineering. Physiochemical nanoparticle assembly techniques involve the application of solvents and potentially harmful chemicals, commonly at high temperatures. Genetically engineered organisms have the potential to be used as promising candidates for greener, efficient, and more adaptable platforms for the synthesis and assembly of nanomaterials. Genetically engineered carriers are precisely designed and constructed in shape and size, enabling precise control over drug attachment sites. The high accuracy of these novel advanced materials, biocompatibility, and stimuli‐responsiveness, elucidate their emerging application in controlled drug delivery. The current article represents the research progress in developing various genetically engineered carriers. Organic‐based nanoparticles including cellulose, collagen, silk‐like polymers, elastin‐like protein, silk‐elastin‐like protein, and inorganic‐based nanoparticles are discussed in detail. Afterward, viral‐based carriers are classified, and their potential for targeted therapeutics delivery is highlighted. Finally, the challenges and prospects of these delivery systems are concluded.

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Elastin‐Like Polymers: Properties, Synthesis, and Applications

Cited by 6 publications

References 252 publications

Elastin-Based Materials: Promising Candidates for Cardiac Tissue Regeneration

Elastin-Based Materials: Promising Candidates for Cardiac Tissue Regeneration

Accessing new biomedical applications by combining genetic design and chemical modification of elastin-like recombinamers

Genetically Engineered Viral Vectors and Organic‐Based Non‐Viral Nanocarriers for Drug Delivery Applications

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