Elastomeric microparticles for acoustic mediated bioseparations

Johnson, Lewis D.; Gao, Lu; Shields, C. Wyatt; Smith, Margret; Efimenko, Kirill; Cushing, Kevin; Genzer, Jan; López, Gabriel P.

doi:10.1186/1477-3155-11-22

Cited by 245 publications

(147 citation statements)

References 21 publications

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“…A photoacoustic flow cytometry system was reported to be able to detect melanoma CTCs by exploiting the broadband absorption spectrum of melanin within CTCs 65 . Recent advancements in the development of elastomer (polymer having both viscosity and elasticity) microparticles bound to target tumor-specific antigens permit the acoustic separations of rare cells based on these properties 88 . Cancer cells are more deformable compared with red blood cells (RBCs) and white blood cells (WBCs) and are able to squeeze through very small pores.…”

Section: Methods: Ctc Isolation Approaches and Molecular Characterizamentioning

confidence: 99%

Using circulating tumor cells to inform on prostate cancer biology and clinical utility

Gregory

García-Blanco

et al. 2015

Critical Reviews in Clinical Laboratory Sciences

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Substantial advances in the molecular biology of prostate cancer have led to the approval of multiple new systemic agents to treat men with metastatic castration-resistant prostate cancer (mCRPC). These treatments encompass androgen receptor directed therapies, immunotherapies, bone targeting radiopharmaceuticals and cytotoxic chemotherapies. There is, however, great heterogeneity in the degree of patient benefit with these agents, thus fueling the need to develop predictive biomarkers that are able to rationally guide therapy. Circulating tumor cells (CTCs) have the potential to provide an assessment of tumor-specific biomarkers through a non-invasive, repeatable “liquid biopsy” of a patient’s cancer at a given point in time. CTCs have been extensively studied in men with mCRPC, where CTC enumeration using the Cellsearch® method has been validated and FDA approved to be used in conjunction with other clinical parameters as a prognostic biomarker in metastatic prostate cancer. In addition to enumeration, more sophisticated molecular profiling of CTCs is now feasible and may provide more clinical utility as it may reflect tumor evolution within an individual particularly under the pressure of systemic therapies. Here, we review technologies used to detect and characterize CTCs, and the potential biological and clinical utility of CTC molecular profiling in men with metastatic prostate cancer.

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Section: Methods: Ctc Isolation Approaches and Molecular Characterizamentioning

confidence: 99%

Using circulating tumor cells to inform on prostate cancer biology and clinical utility

Gregory

García-Blanco

et al. 2015

Critical Reviews in Clinical Laboratory Sciences

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“…27,28 The etched sample also shows these peaks together with a main peak at 1030 cm −1 which is attributed to Si-O-Si stretching vibrations. 29 The minor peak at 975 cm −1 can be assigned to Si-F 2 vibrations. 30,31 According to literature and in agreement with the SIMS results it is also possible that C-F vibrations contribute to this peak (C-F bending at 1100 cm −1 ).…”

Section: Discussionmentioning

confidence: 99%

Stacked Layers of Different Porosity in 4H SiC Substrates Applying a Photoelectrochemical Approach

Leitgeb

Zellner

Hufnagl

et al. 2017

J. Electrochem. Soc.

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Porous 4H-SiC layers were prepared from monocrystalline samples applying photo-electrochemical etching in hydrofluoric acid. The influence of both current and voltage controlled mode during photo-electrochemical porosification was investigated. It was found that the resulting degree of porosity, the homogeneity in porosity as well as the pore morphology mainly depend on the applied voltage, whereas the current level has an almost negligible impact on these important parameters. Based on these results, it is proposed that the formation of porous SiC during photo-electrochemical etching can be described by fractal growth. Finally the gathered knowledge allowed to detach the porous 4H-SiC layers, which comprised several sub-layers of alternating degree of porosity, from the 4H-SiC substrate. Such layers of tailored porosity are key components for several advanced device concepts such as optical filters or membranes for biological applications. A common method to prepare porous Si is electrochemical etching in HF based solutions.1 There are numerous application scenarios of porous Si, such as electrode material in super-capacitors, 2 as sacrificial layer in the production of MEMS devices 3 or as optical filters in bio-or vapor-sensors. 4,5 In the last application scenario, a stacked layer of porous silicon is electrochemically etched into a silicon wafer. The individual porous layers of the stack show alternating degrees of porosity and thus have a different index of refraction. This results in a wavelength selective reflection of optical light.6 Undoubtedly, porous Si is a well-established material for future micro-or nanomachined device architectures, but it shows a poor chemical stability when exposed to e.g. air at higher temperatures or alkaline electrolytes. 7,8 Therefore it has to be covered with a dense protective layer when operation in harsh or biological environments is targeted.9 This drawback encourages research devoted to other porous materials that can be used instead of porous Si without a surface protection. A promising candidate is porous SiC prepared from single crystalline wafers because it shows a higher chemical stability than silicon.10 In contrast to electrochemical etching of Si, photo-electrochemical etching (PECE) of SiC in HF based etching solutions is a comparably challenging task and hence, only a limited number of publications exist.11,12 Some authors report a skin layer on top of the porous structure, which exhibits only a few pores with diameters in the nm range.13,14 This skin layer is followed by a cap layer which shows an irregular porous structure. 15 This problem has been addressed recently by a combination of metal assisted photochemical etching with PECE. 16 Metal assisted photochemical etching (MAPCE) can be utilized to generate a layer in the μm range prior to PECE. The pore tips of this porous layer provide initiation sites for PECE. Thus, neither a skin nor a cap layer are observed.Furthermore some authors report about an increased porosity at the bottom of the porous laye...

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“…1). 93, 94 Using silicone elastomeric particles synthesized from nucleation and growth techniques, we have developed a system where biofunctionalized elastomeric particles decorated with streptavidin can capture cells displaying biotinylated antibodies and confine those cells to the antinodes of an acoustic standing wave (Fig. 7).…”

Section: Bead-based Cell Sortingmentioning

confidence: 99%

Microfluidic cell sorting: a review of the advances in the separation of cells from debulking to rare cell isolation

2015

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Accurate and high throughput cell sorting is a critical enabling technology in molecular and cellular biology, biotechnology, and medicine. While conventional methods can provide high efficiency sorting in short timescales, advances in microfluidics have enabled the realization of miniaturized devices offering similar capabilities that exploit a variety of physical principles. We classify these technologies as either active or passive. Active systems generally use external fields (e.g., acoustic, electric, magnetic, and optical) to impose forces to displace cells for sorting, whereas passive systems use inertial forces, filters, and adhesion mechanisms to purify cell populations. Cell sorting on microchips provides numerous advantages over conventional methods by reducing the size of necessary equipment, eliminating potentially biohazardous aerosols, and simplifying the complex protocols commonly associated with cell sorting. Additionally, microchip devices are well suited for parallelization, enabling complete lab-on-a-chip devices for cellular isolation, analysis, and experimental processing. In this review, we examine the breadth of microfluidic cell sorting technologies, while focusing on those that offer the greatest potential for translation into clinical and industrial practice and that offer multiple, useful functions. We organize these sorting technologies by the type of cell preparation required (i.e., fluorescent label-based sorting, bead-based sorting, and label-free sorting) as well as by the physical principles underlying each sorting mechanism.

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Elastomeric microparticles for acoustic mediated bioseparations

Cited by 245 publications

References 21 publications

Using circulating tumor cells to inform on prostate cancer biology and clinical utility

Using circulating tumor cells to inform on prostate cancer biology and clinical utility

Stacked Layers of Different Porosity in 4H SiC Substrates Applying a Photoelectrochemical Approach

Microfluidic cell sorting: a review of the advances in the separation of cells from debulking to rare cell isolation

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