Electric fields stimulate DNA synthesis of mouse osteoblast‐like cells (MC3T3‐E1) by a mechanism involving calcium ions

Ozawa, Hiroyuki; Abe, Etsuko; Shibasaki, Yoshinobu; Fukuhara, Tatsuo; Suda, Toshio

doi:10.1002/jcp.1041380306

Cited by 75 publications

(48 citation statements)

References 30 publications

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“…PEDOT:PSS (1.3 wt% dispersion in water, PEDOT content 0.5 wt%, PSS content 0.8 wt%, conductive grade), BioReagent Gel (from porcine skin, Type A), phosphate buffered saline (PBS) tablets, tetraethyl orthosilicate (C 8 3 ) were purchased from Sigma-Aldrich (St Louis, MO, USA). The crosslinker 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride was purchased from Acros Organics (Geel, Belgium), and N-hydroxysuccinimide (C 4 H 5 NO 3 ) was purchased from Alfa Aesar (Ward Hill, MA, USA).…”

Section: Materials and Methods Materialsmentioning

confidence: 99%

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3D conductive nanocomposite scaffold for bone tissue engineering

Shahini¹,

Yazdimamaghani²,

Walker³

et al. 2013

IJN

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Bone healing can be significantly expedited by applying electrical stimuli in the injured region. Therefore, a three-dimensional (3D) ceramic conductive tissue engineering scaffold for large bone defects that can locally deliver the electrical stimuli is highly desired. In the present study, 3D conductive scaffolds were prepared by employing a biocompatible conductive polymer, ie, poly(3,4-ethylenedioxythiophene) poly(4-styrene sulfonate) (PEDOT:PSS), in the optimized nanocomposite of gelatin and bioactive glass. For in vitro analysis, adult human mesenchymal stem cells were seeded in the scaffolds. Material characterizations using hydrogen-1 nuclear magnetic resonance, in vitro degradation, as well as thermal and mechanical analysis showed that incorporation of PEDOT:PSS increased the physiochemical stability of the composite, resulting in improved mechanical properties and biodegradation resistance. The outcomes indicate that PEDOT:PSS and polypeptide chains have close interaction, most likely by forming salt bridges between arginine side chains and sulfonate groups. The morphology of the scaffolds and cultured human mesenchymal stem cells were observed and analyzed via scanning electron microscope, micro-computed tomography, and confocal fluorescent microscope. Increasing the concentration of the conductive polymer in the scaffold enhanced the cell viability, indicating the improved microstructure of the scaffolds or boosted electrical signaling among cells. These results show that these conductive scaffolds are not only structurally more favorable for bone tissue engineering, but also can be a step forward in combining the tissue engineering techniques with the method of enhancing the bone healing by electrical stimuli.

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Section: Materials and Methods Materialsmentioning

confidence: 99%

“…2 That can explain why external electric and electromagnetic stimulation have progressive influence in bone healing treatment. [3][4][5] It was shown that such stimulations modify osteoblast activities including adhesion, proliferation, 6 nodule formation, 7 gene expression, 8 protein synthesis, 9 and bone formation markers.…”

Section: Introductionmentioning

confidence: 99%

3D conductive nanocomposite scaffold for bone tissue engineering

Shahini¹,

Yazdimamaghani²,

Walker³

et al. 2013

IJN

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“…They can directly affect osteoclastic cells [54,53], and there is evidence that cells in the osteoblast lineage are sensitive to EMFs [44,46,50]. PEMFs can influence MG63 osteoblast-like cells by increasing transforming growth factor beta-1 (TGF-P1) levels but decreasing levels of prostaglandin E2 (PGE2) in the conditioned media [41].…”

Section: Introductionmentioning

confidence: 99%

Pulsed electromagnetic fields affect phenotype and connexin 43 protein expression in MLO‐Y4 osteocyte‐like cells and ROS 17/2.8 osteoblast‐like cells

Lohmann

Schwartz

Liu

et al. 2003

Journal Orthopaedic Research

120

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Osteocytes, the predominant cells in bone, are postulated to be responsible for sensing mechanical and electrical stimuli, transducing signals via gap junctions. Osteocytes respond to induced shear by increasing connexin 43 (Cx43) levels, suggesting that they might be sensitive to physical stimuli like low-frequency electromagnetic fields (EMF). Immature osteoblasts exhibit decreased intercellular communication in response to EMF but no change in Cx43. Here, we examined long term effects of pulsed EMF (PEMF) on MLO-Y4 osteocyte-like cells and ROS 17/23 osteoblast-like cells. In MLO-Y4 cell cultures, PEMF for 8 h/day for one, two or four days increased alkaline phosphatase activity but had no effect on cell number or osteocalcin. Transforming growth factor beta-1 (TGF-PI) and prostaglandin EZ were increased, and NO2-was altered. PEMFs effect on TGF-Pl was via a prostaglandin-dependent mechanism involving Cox-1 but not Cox-2. In ROS 17/2.X cells, PEMF for 24, 4X or 72 h did not affect cell number, osteocalcin mRNA or osteocalcin protein. PEMF reduced Cx43 protein in both cells. Longer exposures decreased Cx43 mRNA. This indicates that cells in the osteoblast lineage, including well-differentiated osteoblast-like ROS 17/23 cells and terminally differentiated osteocyte-like MLO-Y4 cells, respond to PEMF with changes in local factor production and reduced Cx43, suggesting decreased gap junctional signaling.

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“…Despite much evidence that PEMF can increase the osteoblast proliferation [3][4][5][6][7], differentiation [8][9][10][11] and extracellular matrix calcification [7,8,12,13], there were still some opposite or negative reports [14][15][16][17][18][19]. So results of investigations dealing with this issue are still being debated.…”

Section: Introductionmentioning

confidence: 99%

“…For example, Ozawa et al [4] stimulated murine osteoblast-like cells by a PEMF with an electric field intensity of 3.19 kV/m; McLeod and Collazo [14] exposed MC3T3-E1 cells to a PEMF with an electric field intensity of 0.9 mV/m; Hartig et al [9] used a PEMF with an electric field of 6 kV/m; Chang et al [17] applied a PEMF with an electric field intensity of 0.2 V/m to expose neonatal mouse calvarial bone cell cultures (Table 1). So far there were no reports available on the effects of electromagnetic field with high electric field intensity on bone formation of osteoblasts.…”

Section: Introductionmentioning

confidence: 99%

Inhibition of Bone Formation by High Intensity Pulsed Electromagnetic Field in Mc3t3-E1 Cells

Li¹,

Hui²,

Ma³

et al. 2011

PIER

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Abstract-To investigate the effects of pulsed electromagnetic field (PEMF) with high electric field intensity on bone formation in murine osteoblast-like MC3T3-E1 cells, proliferation, alkaline phosphotase (ALP) activity, mineralized nodule formation, Collagen Type I (COL-I) and core-binding factor (Cbf)a1 mRNA expression, and bone morphogenetic protein (BMP)2/4 and mothers against decapentaplegic (Smad)1/5/8 protein expression were examined in cultured MC3T3-E1 cells after exposure to PEMF at the field intensities of 0 kV/m, 50 kV/m or 400 kV/m for 400 consecutive pulses daily for 7 consecutive days. After 50 kV/m of PEMF exposure, none of the above parameters of MC3T3-E1 cells changed significantly when compared to the control groups. However, the proliferation, ALP activity and mineralized nodule formation of MC3T3-E1 cells in 400 kV/m PEMF exposure groups decreased significantly although COL-I and Cbfa1 mRNA expression and BMP2/4 and Smad1/5/8 protein expression did not change. The PEMF we used at high electric field intensity suppressed proliferation, differentiation and

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Electric fields stimulate DNA synthesis of mouse osteoblast‐like cells (MC3T3‐E1) by a mechanism involving calcium ions

Cited by 75 publications

References 30 publications

3D conductive nanocomposite scaffold for bone tissue engineering

3D conductive nanocomposite scaffold for bone tissue engineering

Pulsed electromagnetic fields affect phenotype and connexin 43 protein expression in MLO‐Y4 osteocyte‐like cells and ROS 17/2.8 osteoblast‐like cells

Inhibition of Bone Formation by High Intensity Pulsed Electromagnetic Field in Mc3t3-E1 Cells

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