Electrical detection of DNA immobilization and hybridization by streaming current measurements in microchannels

Abstract

Label-free electrical detection of surface DNA immobilization and hybridization via streaming current measurements in a microchannel is demonstrated. Streaming currents generated by the flow of deionised water through a polydimethysiloxane microchannel sealed on glass are measured using integrated Au electrodes and are sensitive to the density and polarity of the charge on the channel surface. An in-channel DNA hybridization protocol was developed. Streaming currents were monitored after each of protocol steps. The technique was applied to label free recognition of DNA hybridization and could distinguish between assays with complementary and non-complementary DNA strands.

Streaming Current Based Microtubular Enzymatic Sensor for Self‐Powered Detection of Urea

“…The study of flow‐induced electrification has offered exciting prospects of developing innovative microfluidic sensors for many chemical and biological applications. The contact between the electrolyte solution and the microfluidic channel brings about a charge abundant electrical double layer (EDL) at the liquid–solid interface, triggering electrical signals which are sensitive to the flow rate and ion concentration .…”

“…Besides, microchips embedded with carbon nanotubes or graphene transistors were developed to monitor flow rate and ion concentration by locally measuring the streaming potential . Label‐free detection of DNA immobilization and hybridization was also realized by monitoring the change in the streaming current . However, despite these achievements, selective detection of analytes is extremely challenging for microfluidic sensors based on flow‐induced electrification, owing to the lack of recognition elements in the flow pathway.…”

Gap tuning and effective electron correlation energy in amorphous silicon: A first principles density functional theory-based molecular dynamics study

“…For example, in thin film a-Si light sensors the emitted light is converted into an electrical signal, thus allowing the optoelectronic detection of the DNA molecules. Whereas, in ion-sensitive thin film a-Si transistors the induced voltage shifts are the basis of detection for sensing of DNA and proteins [4][5][6]. The gap tuning technology in a-Si can control the accuracy of the functionality of these devices.…”

Electrical sensing of DNA-hybridization using two-port network based on suspended carbon nanotube membrane

“…Methods for label-free DNA hybridization detection are in demand because they are simple, fast, and able to realize real-time, in-situ detection. They are currently achieved mainly by optical (Bougot-Robin et al 2014;Toccafondo et al 2010;Özkumur et al 2010;Suter et al 2008;Zhu et al 2014), mechanical (Ramos et al 2009;Su et al 2003;Ilic et al 2005;Huber et al 2013;Zuniga et al 2009;, and electrical (Zebda et al 2010;Paleček et al 1998;Zaffino et al 2014;Sensors et al 2012;Khan et al 2010;Martins et al 2011;Archer et al 2004) technologies.…”