Electrical Modeling of the Growth and Differentiation of Skeletal Myoblasts Cell Cultures for Tissue Engineering

Olmo, Alberto; Yuste, Yaiza; Serrano, Juan Alfonso; Maldonado-Jacobi, Andrés; Pérez, Pablo; Huertas, Gloria; Pereira, Sheila M.; Yufera, A.; Portilla, Fernando de la

doi:10.3390/s20113152

Cited by 8 publications

(8 citation statements)

References 22 publications

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“…The use of electrical impedance spectroscopy has been studied for cell culture monitoring in different works for a wide variety of applications, such as the study of cell proliferation [1,[27][28][29], cell toxicity [30], or cellular differentiation [31]. In [28], an oscillation-based circuit was proposed for the measurement of electrical impedance in cell cultures.…”

Section: Range Of Porementioning

confidence: 99%

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Characterization and Monitoring of Titanium Bone Implants with Impedance Spectroscopy

Olmo

Hernández

Chicardi

et al. 2020

Sensors

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Porous titanium is a metallic biomaterial with good properties for the clinical repair of cortical bone tissue, although the presence of pores can compromise its mechanical behavior and clinical use. It is therefore necessary to characterize the implant pore size and distribution in a suitable way. In this work, we explore the new use of electrical impedance spectroscopy for the characterization and monitoring of titanium bone implants. Electrical impedance spectroscopy has been used as a non-invasive route to characterize the volumetric porosity percentage (30%, 40%, 50% and 60%) and the range of pore size (100–200 and 355–500 mm) of porous titanium samples obtained with the space-holder technique. Impedance spectroscopy is proved to be an appropriate technique to characterize the level of porosity of the titanium samples and pore size, in an affordable and non-invasive way. The technique could also be used in smart implants to detect changes in the service life of the material, such as the appearance of fractures, the adhesion of osteoblasts and bacteria, or the formation of bone tissue.

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Section: Range Of Porementioning

confidence: 99%

“…Figure 6a shows the equivalent model proposed in [28] for modeling the electrical behavior of the cell culture and instrumentation system. toxicity [30], or cellular differentiation [31]. In [28], an oscillation-based circuit was proposed for the measurement of electrical impedance in cell cultures.…”

Section: Range Of Porementioning

confidence: 99%

Characterization and Monitoring of Titanium Bone Implants with Impedance Spectroscopy

Olmo

Hernández

Chicardi

et al. 2020

Sensors

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“…Two-dimensional cell cultures have been traditionally studied with electrical impedance, as previously mentioned, for diverse applications, such as cell toxicity assessment [ 3 ], the study of cell motility [ 4 ], or tissue engineering [ 6 , 7 ]. In the study of impedance spectroscopy measurements of 2D cell cultures, the use of 3D printing technologies for the implementation of a cell culture device is proposed in Cabrera-López et al [ 12 ].…”

Section: 3d-printed Sensors In Cell Culture and Tissue Engineeringmentioning

confidence: 99%

“…Among the different technologies traditionally used for sensing cell cultures, electrical impedance measurements are one of the most frequently used [ 2 ]. Electrical impedance measurements have long been used in biomedical engineering [ 2 ] for different applications, such as cell toxicity assessment [ 3 ], the study of cell motility [ 4 ], analysis of cancer cell lines [ 5 ] or tissue engineering [ 6 , 7 ], among others. Electrical cell-impedance sensing, or ECIS, aims at measuring cell impedance, providing information about cell morphology and electric properties, including cell proliferation, attachment, migration, barrier function or cytoplasm conductivity [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

3D-Printed Sensors and Actuators in Cell Culture and Tissue Engineering: Framework and Research Challenges

Pérez

Serrano

Olmo

2020

Sensors

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Three-dimensional printing technologies have been recently proposed to monitor cell cultures and implement cell bioreactors for different biological applications. In tissue engineering, the control of tissue formation is crucial to form tissue constructs of clinical relevance, and 3D printing technologies can also play an important role for this purpose. In this work, we study 3D-printed sensors that have been recently used in cell culture and tissue engineering applications in biological laboratories, with a special focus on the technique of electrical impedance spectroscopy. Furthermore, we study new 3D-printed actuators used for the stimulation of stem cells cultures, which is of high importance in the process of tissue formation and regenerative medicine. Key challenges and open issues, such as the use of 3D printing techniques in implantable devices for regenerative medicine, are also discussed.

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“…Moreover, changes in regulatory factors or protein within cells affected cell impedance, for instance, vinculin-null cells exhibited lower cell impedance [ 4 ]; Amyloid-β 42 regulates cell adhesion via the TJ protein to regulate impedance of MDCK cells [ 5 ]. The ECIS technique provided useful information for estimating the fill factor of muscular stem cells [ 6 ]. This technique was also used in previous works to study tumor cell cycle [ 7 ], invasion [ 8 ], proliferation [ 9 ], and the interaction between tumor cells and other stromal cells [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

The Measurement and Analysis of Impedance Response of HeLa Cells to Distinct Chemotherapy Drugs

Kong

Liu

et al. 2021

Micromachines

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Electric cell–substrate impedance sensing exhibits a real-time and label-free feature to monitor the response of cells stimulated by various biochemical and mechanical signals. Alterations in the currents passing through the cell–electrode system characterize the impedance variations of cells. The impedance responses of HeLa cells under distinct chemotherapy drugs combine the effects of cell proliferation and cell–substrate adhesion. Optimal interdigitated electrodes were selected to explore the impedance responses of HeLa cells. Measurements of impedance of cells in response to three widely used chemotherapy drugs in clinical practice, namely cisplatin, doxorubicin, 5-fluorouracil, were performed. The results demonstrated that distinct impedance responses of HeLa cells to drugs were exhibited and a decrease in measured impedance was observed after drug treatment, accompanied by alterations in the distribution and intensity of the adhesion-related protein vinculin and the rate of cell proliferation. The link between the impedance profiles of HeLa cells and their biological functions was developed based on the circuit model. This study demonstrated the weights of cell proliferation and adhesion of HeLa cells under the treatments of DDP, DOX, and 5-FU, resulted in distinct impedance responses of cells, providing an impedance-based evaluation methodology for cervical cancer treatment.

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Electrical Modeling of the Growth and Differentiation of Skeletal Myoblasts Cell Cultures for Tissue Engineering

Cited by 8 publications

References 22 publications

Characterization and Monitoring of Titanium Bone Implants with Impedance Spectroscopy

Characterization and Monitoring of Titanium Bone Implants with Impedance Spectroscopy

3D-Printed Sensors and Actuators in Cell Culture and Tissue Engineering: Framework and Research Challenges

The Measurement and Analysis of Impedance Response of HeLa Cells to Distinct Chemotherapy Drugs

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