Electrical regulation of Schwann cells using conductive polypyrrole/chitosan polymers

Huang, Jinghui; Hu, Xueyu; Lü, Lei; Ye, Zhengxu; Zhang, Quanyu; Luo, Zhuojing

doi:10.1002/jbm.a.32511

Cited by 184 publications

(136 citation statements)

References 35 publications

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“…Collagen-PPy hybrid material prepared by the chemical polymerization of pyrrole, using FeCl 3 as initiator, was also carried out by these researchers in the presence of soluble collagen (Li and Khor, 1994). Huang et al (2010) fabricated a PPy-chitosan membrane, seeded Schwann cells on the scaffolds and showed that the PPy-chitosan membranes supported cell adhesion and proliferation, indicating its biocompatibility. We carried out the electrospinning of a blend of poly(ε-caprolactone) (PCL), gelatin and PANI to obtain conductive nanofibrous scaffolds for nerve tissue engineering.…”

Section: Modification Of Conductive Materials With Biopolymersmentioning

confidence: 99%

“…They concluded that electrical stimulation using clinically acceptable waveforms has a significant effect on the rate of release of both proteins (NT-3 and BDNF). Electrical stimulation has also been applied to Schwann cells cultured on polypyrrole-chitosan membrane (Huang et al, 2010). Constant potential gradient (100 mV/mm) was applied to the cells through the membrane for 4 h and then the cells were incubated for an additional 12, 24 or 36 h. The results showed that electrical stimulation applied through conductive PPy-chitosan significantly enhanced the viability of Schwann cells and dramatically enhanced secretion of NGF and brain-derived neurotrophic factor (BDNF) and protein expression.…”

Section: Application Of Electrical Stimulation In Nerve Tissue Enginementioning

confidence: 99%

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Application of conductive polymers, scaffolds and electrical stimulation for nerve tissue engineering

Ghasemi‐Mobarakeh¹,

Prabhakaran²,

Morshed³

et al. 2011

J Tissue Eng Regen Med

616

389

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Among the numerous attempts to integrate tissue engineering concepts into strategies to repair nearly all parts of the body, neuronal repair stands out. This is partially due to the complexity of the nervous anatomical system, its functioning and the inefficiency of conventional repair approaches, which are based on single components of either biomaterials or cells alone. Electrical stimulation has been shown to enhance the nerve regeneration process and this consequently makes the use of electrically conductive polymers very attractive for the construction of scaffolds for nerve tissue engineering. In this review, by taking into consideration the electrical properties of nerve cells and the effect of electrical stimulation on nerve cells, we discuss the most commonly utilized conductive polymers, polypyrrole (PPy) and polyaniline (PANI), along with their design and modifications, thus making them suitable scaffolds for nerve tissue engineering. Other electrospun, composite, conductive scaffolds, such as PANI/gelatin and PPy/poly(ε-caprolactone), with or without electrical stimulation, are also discussed. Different procedures of electrical stimulation which have been used in tissue engineering, with examples on their specific applications in tissue engineering, are also discussed.

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Section: Modification Of Conductive Materials With Biopolymersmentioning

confidence: 99%

Section: Application Of Electrical Stimulation In Nerve Tissue Enginementioning

confidence: 99%

Application of conductive polymers, scaffolds and electrical stimulation for nerve tissue engineering

Ghasemi‐Mobarakeh¹,

Prabhakaran²,

Morshed³

et al. 2011

J Tissue Eng Regen Med

616

389

View full text Add to dashboard Cite

show abstract

“…These cells play a key role in nerve regeneration by secreting neurotrophic factors that help the axon sprouts grow towards the injured neurons 62 . Several reports demonstrated that the secretion/expression of both brainderived neurotrophic factor and neuron growth factor (NGF) were elevated on electrical stimulation [63][64][65] . Similar to the situation described above, our poly(EDOT-MI-co-EDOT-PC) films were inert towards the Schwann cells in the absence of attached ligands.…”

Section: Articlementioning

confidence: 99%

Large enhancement in neurite outgrowth on a cell membrane-mimicking conducting polymer

et al. 2014

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Although electrically stimulated neurite outgrowth on bioelectronic devices is a promising means of nerve regeneration, immunogenic scar formation can insulate electrodes from targeted cells and tissues, thereby reducing the lifetime of the device. Ideally, an electrode material capable of electrically interfacing with neurons selectively and efficiently would be integrated without being recognized by the immune system and minimize its response. Here we develop a cell membrane-mimicking conducting polymer possessing several attractive features. This polymer displays high resistance towards nonspecific enzyme/cell binding and recognizes targeted cells specifically to allow intimate electrical communication over long periods of time. Its low electrical impedance relays electrical signals efficiently. This material is capable to integrate biochemical and electrical stimulation to promote neural cellular behaviour. Neurite outgrowth is enhanced greatly on this new conducting polymer; in addition, electrically stimulated secretion of proteins from primary Schwann cells can also occur on it.

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“…include transmitting electric impulses in neurite cultures, 12 regenerating nerves, 13 and healing wounds. 11 Cardiac applications to synchronize contractility in the injured myocardium have been suggested, but not functionally tested.…”

mentioning

confidence: 99%

A Conductive Polymer Hydrogel Supports Cell Electrical Signaling and Improves Cardiac Function After Implantation into Myocardial Infarct

et al. 2015

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C oronary heart disease is the leading cause of the rising incidence of heart failure worldwide.1 Following myocardial infarction (MI), the limited regenerative potential of the heart causes scar formation in and around the infarction, leading to abnormal electric signal propagation and desynchronized cardiac activation and contraction.2 The lack of electric connection between healthy myocardium and the scar with its islands of intact cardiomyocytes contributes to progressive functional decompensation. Injectable biomaterial has shown promise as an alternative biological treatment option after MI to reduce adverse remodeling and preserve cardiac function.3,4 Among their many advantages, injectable materials can be delivered alone or as a vehicle carrying combination therapies, including cells or growth factors, and may provide mechanical and functional support to the injured heart. Over the past decade, several injectable biomaterials such as collagen, 5,6 alginate, 7 and fibrin 8 have been extensively studied. Organic polymers that conduct electricity (conductive polymers) were first described in 1977, and this discovery was awarded the Nobel prize in 2000.9,10 Conductive polymers are particularly appealing because they exhibit electric properties similar to metals and semiconductors while retaining flexibility, ease of processing, and modifiable conductivity. The electric properties of these materials can be fine-tuned by altering their synthetic processes, including the addition of specific chemical agents.11 Biological applications of conductive polymersBackground-Efficient cardiac function requires synchronous ventricular contraction. After myocardial infarction, the nonconductive nature of scar tissue contributes to ventricular dysfunction by electrically uncoupling viable cardiomyocytes in the infarct region. Injection of a conductive biomaterial polymer that restores impulse propagation could synchronize contraction and restore ventricular function by electrically connecting isolated cardiomyocytes to intact tissue, allowing them to contribute to global heart function. Methods and Results-We created a conductive polymer by grafting pyrrole to the clinically tested biomaterial chitosan to create a polypyrrole (PPy)-chitosan hydrogel. Cyclic voltammetry showed that PPy-chitosan had semiconductive properties lacking in chitosan alone. PPy-chitosan did not reduce cell attachment, metabolism, or proliferation in vitro. Neonatal rat cardiomyocytes plated on PPy-chitosan showed enhanced Ca 2+ signal conduction in comparison with chitosan alone. PPy-chitosan plating also improved electric coupling between skeletal muscles placed 25 mm apart in comparison with chitosan alone, demonstrating that PPy-chitosan can electrically connect contracting cells at a distance. In rats, injection of PPy-chitosan 1 week after myocardial infarction decreased the QRS interval and increased the transverse activation velocity in comparison with saline or chitosan, suggesting improved electric conduction. Optical mapping showed incr...

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Electrical regulation of Schwann cells using conductive polypyrrole/chitosan polymers

Cited by 184 publications

References 35 publications

Application of conductive polymers, scaffolds and electrical stimulation for nerve tissue engineering

Application of conductive polymers, scaffolds and electrical stimulation for nerve tissue engineering

Large enhancement in neurite outgrowth on a cell membrane-mimicking conducting polymer

A Conductive Polymer Hydrogel Supports Cell Electrical Signaling and Improves Cardiac Function After Implantation into Myocardial Infarct

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