Electrical stimulation-induced osteogenesis of human adipose derived stem cells using a conductive graphene-cellulose scaffold

Li, Jianfeng; Liu, Xiao; Crook, Jeremy Micah; Wallace, Gordon G.

doi:10.1016/j.msec.2019.110312

Cited by 54 publications

(43 citation statements)

References 46 publications

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“…Reproduced with permission. [98] Copyright 2019, Elsevier. B) Charge injection cell culture set up where the conductive biomaterial is the working electrode, and an anode is submerged into the culture media.…”

Section: Conductive Biomaterialsmentioning

confidence: 99%

“…Electrical stimulation of multipotent stem cells, specifically MSC and adipose stem cells (ASC), primarily are reported to enhance osteogenic differentiation, [98,120,123,125] as well as smooth muscle cells as investigated by Björninen et al [122] Zhang et al Figure 9. A) ALP activity on PPy-II electrodes with different Q inj and B) ALP activity on PPy-I and PPy-III electrodes with Q inj of 0.08 µC.…”

Section: Cellular Response To Electrical Stimulationmentioning

confidence: 99%

See 1 more Smart Citation

Stimuli‐Responsive Biomaterials: Scaffolds for Stem Cell Control

Gelmi

Schutt

2020

Adv Healthcare Materials

114

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Stem cell fate is closely intertwined with microenvironmental and endogenous cues within the body. Recapitulating this dynamic environment ex vivo can be achieved through engineered biomaterials which can respond to exogenous stimulation (including light, electrical stimulation, ultrasound, and magnetic fields) to deliver temporal and spatial cues to stem cells. These stimuli‐responsive biomaterials can be integrated into scaffolds to investigate stem cell response in vitro and in vivo, and offer many pathways of cellular manipulation: biochemical cues, scaffold property changes, drug release, mechanical stress, and electrical signaling. The aim of this review is to assess and discuss the current state of exogenous stimuli‐responsive biomaterials, and their application in multipotent stem cell control. Future perspectives in utilizing these biomaterials for personalized tissue engineering and directing organoid models are also discussed.

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Section: Conductive Biomaterialsmentioning

confidence: 99%

Section: Cellular Response To Electrical Stimulationmentioning

confidence: 99%

Stimuli‐Responsive Biomaterials: Scaffolds for Stem Cell Control

Gelmi

Schutt

2020

Adv Healthcare Materials

114

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“…Compared with traditional metal electrodes, graphene-containing composites had lower impedance and higher charge injection capacity. For example, Li et al 92 prepared GDs-cellulose (G-C) scaffold and assembled an electrode device on this basis, which provided a platform for support and osteogenic differentiation of adipose-derived stem cell (ADSC). The results showed that the charge storage capacity of G-C electrode (0.966 mC/cm 2 ) was higher than that of gold electrode (0.462 mC/cm 2 ).…”

Section: Excellent Electrical Propertiesmentioning

confidence: 99%

<p>Applications of Graphene and Its Derivatives in Bone Repair: Advantages for Promoting Bone Formation and Providing Real-Time Detection, Challenges and Future Prospects</p>

Du¹,

Wang

Zhang³

et al. 2020

IJN

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During continuous innovation in the preparation, characterization and application of various bone repair materials for several decades, nanomaterials have exhibited many unique advantages. As a kind of representative two-dimensional nanomaterials, graphene and its derivatives (GDs) such as graphene oxide and reduced graphene oxide have shown promising potential for the application in bone repair based on their excellent mechanical properties, electrical conductivity, large specific surface area (SSA) and atomic structure stability. Herein, we reviewed the updated application of them in bone repair in order to present, as comprehensively, as possible, their specific advantages, challenges and current solutions. Firstly, how their advantages have been utilized in bone repair materials with improved bone formation ability was discussed. Especially, the effects of further functionalization or modification were emphasized. Then, the signaling pathways involved in GDs-induced osteogenic differentiation of stem cells and immunomodulatory mechanism of GDs-induced bone regeneration were discussed. On the other hand, their applications as contrast agents in the field of bone repair were summarized. In addition, we also reviewed the progress and related principles of the effects of GDs parameters on cytotoxicity and residues. At last, the future research was prospected.

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“…Graphene is being intensely researched globally for applications in fields from electronics to medicine (Geim and Novoselov, 2007;Thompson et al, 2015;Ke and Wang, 2016;Kandyba et al, 2018;Li et al, 2019b). In medicine, the realization of 3D cell-supporting structures containing graphene has significant potential for tissue engineering and replacement.…”

Section: Introductionmentioning

confidence: 99%

3D Printing of Cytocompatible Graphene/Alginate Scaffolds for Mimetic Tissue Constructs

Liu

Crook

et al. 2020

Front. Bioeng. Biotechnol.

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Tissue engineering, based on a combination of 3D printing, biomaterials blending and stem cell technology, offers the potential to establish customized, transplantable autologous implants using a patient's own cells. Graphene, as a two-dimensional (2D) version of carbon, has shown great potential for tissue engineering. Here, we describe a novel combination of graphene with 3D printed alginate (Alg)-based scaffolds for human adipose stem cell (ADSC) support and osteogenic induction. Alg printing was enabled through addition of gelatin (Gel) that was removed after printing, and the 3D structure was then coated with graphene oxide (GO). GO was chemically reduced with a biocompatible reductant (ascorbic acid) to provide electrical conductivity and cell affinity sites. The reduced 3D graphene oxide (RGO)/Alg scaffold has good cytocompatibility and can support human ADSC proliferation and osteogenic differentiation. Our finding supports the potential for the printed scaffold's use for in vitro engineering of bone and other tissues using ADSCs and potentially other human stem cells, as well as in vivo regenerative medicine.

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Electrical stimulation-induced osteogenesis of human adipose derived stem cells using a conductive graphene-cellulose scaffold

Cited by 54 publications

References 46 publications

Stimuli‐Responsive Biomaterials: Scaffolds for Stem Cell Control

Stimuli‐Responsive Biomaterials: Scaffolds for Stem Cell Control

<p>Applications of Graphene and Its Derivatives in Bone Repair: Advantages for Promoting Bone Formation and Providing Real-Time Detection, Challenges and Future Prospects</p>

3D Printing of Cytocompatible Graphene/Alginate Scaffolds for Mimetic Tissue Constructs

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