Electrical Stimulation of Human Mesenchymal Stem Cells on Conductive Nanofibers Enhances their Differentiation toward Osteogenic Outcomes

Hardy, John G.; Villancio-Wolter, Maria K.; Sukhavasi, Rushi C.; Mouser, David J.; Aguilar, David; Geissler, Sydney A.; Kaplan, David L.; Schmidt, Christine E.

doi:10.1002/marc.201500233

Cited by 54 publications

(41 citation statements)

References 87 publications

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“…Electrical stimulation of stem cells seeded on Ppy scaffolds can also drive osteogenesis [245] and the formation of functional muscle tissues [246]. Ppy can also be electrospun to produce scaffolds for stem cell differentiation [247] as well as be combined with other materials to generate conductive hybrid scaffolds for stem cell-based tissue engineering [248].…”

Section: Polypyrrole (Ppy)mentioning

confidence: 99%

Combining Stem Cells and Biomaterial Scaffolds for Constructing Tissues and Cell Delivery

Willerth

Sakiyama‐Elbert

2019

STJ

114

113

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Combining stem cells with biomaterial scaffolds serves as a promising strategy for engineering tissues for both in vitro and in vivo applications. This updated review details commonly used biomaterial scaffolds for engineering tissues from stem cells. We first define the different types of stem cells and their relevant properties and commonly used scaffold formulations. Next, we discuss natural and synthetic scaffold materials typically used when engineering tissues, along with their associated advantages and drawbacks and gives examples of target applications. New approaches to engineering tissues, such as 3D bioprinting, are described as they provide exciting opportunities for future work along with current challenges that must be addressed. Thus, this review provides an overview of the available biomaterials for directing stem cell differentiation as a means of producing replacements for diseased or damaged tissues.

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Section: Polypyrrole (Ppy)mentioning

confidence: 99%

Combining Stem Cells and Biomaterial Scaffolds for Constructing Tissues and Cell Delivery

Willerth

Sakiyama‐Elbert

2019

STJ

114

113

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“…While the mechanism by which ES promotes bone healing is still poorly understood, recent in vitro studies show that bioelectrical signals play a key role in cellular pathways involved in healing (Levin, 2009). Recently, in vitro studies showed that DC ES, acting partially via electrochemical reaction at the cathode, alters several MSC behaviors, such as migration, proliferation, and differentiation (Hardy et al, 2015; Tandon et al, 2009b; Tsai et al, 2009; Zhao et al, 2011). Specifically, correlations between ES and the rate of osteogenic differentiation have been reported (Fukada & Yasuda, 1957; Shamos, Lavine & Shamos, 1963).…”

Section: Introductionmentioning

confidence: 99%

In vitroeffect of direct current electrical stimulation on rat mesenchymal stem cells

Mobini

Leppik

Parameswaran

et al. 2017

PeerJ

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BackgroundElectrical stimulation (ES) has been successfully used to treat bone defects clinically. Recently, both cellular and molecular approaches have demonstrated that ES can change cell behavior such as migration, proliferation and differentiation.MethodsIn the present study we exposed rat bone marrow- (BM-) and adipose tissue- (AT-) derived mesenchymal stem cells (MSCs) to direct current electrical stimulation (DC ES) and assessed temporal changes in osteogenic differentiation. We applied 100 mV/mm of DC ES for 1 h per day for three, seven and 14 days to cells cultivated in osteogenic differentiation medium and assessed viability and calcium deposition at the different time points. In addition, expression of osteogenic genes, Runx2, Osteopontin, and Col1A2 was assessed in BM- and AT-derived MSCs at the different time points.ResultsResults showed that ES changed osteogenic gene expression patterns in both BM- and AT-MSCs, and these changes differed between the two groups. In BM-MSCs, ES caused a significant increase in mRNA levels of Runx2, Osteopontin and Col1A2 at day 7, while in AT-MSCs, the increase in Runx2 and Osteopontin expression were observed after 14 days of ES.DiscussionThis study shows that rat bone marrow- and adipose tissue-derived stem cells react differently to electrical stimuli, an observation that could be important for application of electrical stimulation in tissue engineering.

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“…A variety of CP‐based biomaterials have been synthesized using polypyrrole (PPy), polythiophene (PT), poly(3,4‐ethylenedioxythiophene) (PEDOT), or polyaniline (PANi) . These conductive biomaterials can be used to support and efficiently influence responses of various cells, including electrically excitable cells (e.g., neurons, cardiomyocytes, and myoblasts) and even stem cells, for potential regeneration of diseased or injured tissues …”

Section: Introductionmentioning

confidence: 99%

Polypyrrole/Alginate Hybrid Hydrogels: Electrically Conductive and Soft Biomaterials for Human Mesenchymal Stem Cell Culture and Potential Neural Tissue Engineering Applications

et al. 2016

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Electrically conductive biomaterials that can efficiently deliver electrical signals to cells or improve electrical communication among cells have received considerable attention for potential tissue engineering applications. Conductive hydrogels are desirable particularly for neural applications, as they can provide electrical signals and soft microenvironments that can mimic native nerve tissues. In this study, conductive and soft polypyrrole/alginate (PPy/Alg) hydrogels are developed by chemically polymerizing PPy within ionically cross-linked alginate hydrogel networks. The synthesized hydrogels exhibit a Young's modulus of 20-200 kPa. Electrical conductance of the PPy/Alg hydrogels could be enhanced by more than one order of magnitude compared to that of pristine alginate hydrogels. In vitro studies with human bone marrow-derived mesenchymal stem cells (hMSCs) reveal that cell adhesion and growth are promoted on the PPy/Alg hydrogels. Additionally, the PPy/Alg hydrogels support and greatly enhance the expression of neural differentiation markers (i.e., Tuj1 and MAP2) of hMSCs compared to tissue culture plate controls. Subcutaneous implantation of the hydrogels for eight weeks induces mild inflammatory reactions. These soft and conductive hydrogels will serve as a useful platform to study the effects of electrical and mechanical signals on stem cells and/or neural cells and to develop multifunctional neural tissue engineering scaffolds.

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Electrical Stimulation of Human Mesenchymal Stem Cells on Conductive Nanofibers Enhances their Differentiation toward Osteogenic Outcomes

Cited by 54 publications

References 87 publications

Combining Stem Cells and Biomaterial Scaffolds for Constructing Tissues and Cell Delivery

Combining Stem Cells and Biomaterial Scaffolds for Constructing Tissues and Cell Delivery

In vitroeffect of direct current electrical stimulation on rat mesenchymal stem cells

Polypyrrole/Alginate Hybrid Hydrogels: Electrically Conductive and Soft Biomaterials for Human Mesenchymal Stem Cell Culture and Potential Neural Tissue Engineering Applications

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