Electrically detected displacement assay (EDDA): a practical approach to nucleic acid testing in clinical or medical diagnosis

Liepold, Petra; Kratzmüller, T.; Persike, N.; Bandilla, Michael; Hinz, Michael; Wieder, H.; Hillebrandt, Heiko; Ferrer, Elisenda; Hartwich, Gerhard

doi:10.1007/s00216-008-2045-5

Cited by 49 publications

(50 citation statements)

References 35 publications

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“…Using covalent ferrocene labels, Hartwich et al also found similar melting behavior [34]. The effect of the osmium marker upon the melting temperature should be small since the double stranded section did not contain labeled bases.…”

Section: Resultsmentioning

confidence: 83%

“…However, these first approaches either required sophisticated probe-SAM design on the electrode or difficult calculation of very small signal current differences, which were depending upon the hybridization temperature. More promising are approaches basing upon covalent DNA labels such as ferrocene and osmium(II) complexes, which are, however, costly modifications [34].…”

Section: Introductionmentioning

confidence: 99%

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Electrochemical Detection of DNA Melting Curves by Means of Heated Biosensors

Surkus¹,

Flechsig²

2009

Electroanalysis

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This article reports about the detection of DNA melting curves at heated electrochemical biosensors. Osmium tetroxide-bipyridine-labeled target oligonucleotides are hybridized with probe oligonucleotides immobilized on gold electrodes. Then, the gold electrode is successively heated in order to measure a complete melting curve consisting of alternating current voltammetric signals. Melting temperatures W m , determined at various ionic strengths and in dependence on different numbers of base pair mismatches, have been compared with those obtained by means of UV spectrophotometry. The proposed method holds great promise for the fast and easy parallel detection of nucleic acids sequences on selectively heated electrode arrays. A stringent hybridization temperature can be easily adjusted in order to discriminate base pair mismatches.

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Section: Resultsmentioning

confidence: 83%

Section: Introductionmentioning

confidence: 99%

Electrochemical Detection of DNA Melting Curves by Means of Heated Biosensors

Surkus¹,

Flechsig²

2009

Electroanalysis

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“…6B). This may result from insufficient removal of nonspecifically adsorbed cDNA molecules or nonselective detachment of cDNA molecules due to the relatively weak bond between thiol and gold [19]. The nonselective recovery of genetic molecules from a chip causes a noise of selected genetic information and disturbs an efficient selection of functional biomolecules.…”

Section: Photorecovery and Amplification Of Cdnamentioning

confidence: 99%

Photoassisted Recovery of DNA Molecules for On-chip Directed Evolution

Ueno

Ono

Tanaka

et al. 2012

J. Photopol. Sci. Technol.

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Directed evolution is a powerful approach to creating functional biomolecules, and a combinative use of microarray chip technology is expected to have a great potential for realizing a sophisticated directed evolution with higher speed and quantitative performance than ever before. Although "recovery" of evaluated and selected molecules followed by amplification is an essential step in the performance of directed evolution, conventional DNA or protein microarray chips are limited only to analytical use. In this study, we have developed a novel technology for DNA microarray that is applicable to an artificial Darwinian selection on a chip. DNA molecules were microarrayed on a gold surface via an oligonucleotide linker that contains a nitrobenzyl group as a photocleavable moiety and were recovered from the surface by spot-selective photoirradiation at few micrometer precision. The recovered DNA molecules were confirmed by polymerase chain reaction. Thus, in this study, we have realized a new function of biomolecular microarray technology that is useful for the establishment of on-chip directed molecular evolution.

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“…Particularly, SiNWs have recently attracted attention in the research community due to label-free detection with scope for mass production, multi detection, CMOS compatibility and portability [5]. Contemporary DNA sensors employ optical readout techniques and use labels for hybridization thus making them expensive and bulky [6]. By eliminating optical readout-hybridization techniques and enabling direct electrical detection it is possible to make DNA sensing label free.…”

Section: Introductionmentioning

confidence: 99%

Silicon nanowires integrated with CMOS circuits for biosensing application

Jayakumar

Asadollahi

Hellström

et al. 2014

Solid-State Electronics

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a b s t r a c tWe describe a silicon nanowire (SiNW) biosensor fabricated in a fully depleted SOI CMOS process. The sensor array consists of N by N pixel matrix (N 2 pixels or test sites) and 8 input-output (I/O) pins. In each pixel a single crystalline SiNW with 75 by 20 nm cross-section area is defined using sidewall transfer lithography in the SOI layer. The key advantage of the design is that each individual SiNWs can be read-out sequentially and used for real-time charge based detection of molecules in liquids or gases.

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Electrically detected displacement assay (EDDA): a practical approach to nucleic acid testing in clinical or medical diagnosis

Cited by 49 publications

References 35 publications

Electrochemical Detection of DNA Melting Curves by Means of Heated Biosensors

Electrochemical Detection of DNA Melting Curves by Means of Heated Biosensors

Photoassisted Recovery of DNA Molecules for On-chip Directed Evolution

Silicon nanowires integrated with CMOS circuits for biosensing application

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