Electro‐membrane process for the separation of amino acids by iso‐electric focusing

Abstract

BACKGROUND: Amino acids (AAs) are usually produced commercially using chemical, biochemical and microbiological fermentation methods. The product obtained from these methods undergoes various treatments involving extraction and electrodialysis (ED) for salt removal and AA recovery. This paper describes an electro-membrane process (EMP) for the charge based separation of amino acids.

Selective electromembrane extraction based on isoelectric point: Fundamental studies with angiotensin II antipeptide as model analyte

“…This was considered as a high recovery, although a small fraction of the analyte was lost in the process. The three positively charged peptides AT2, NT and AT1 were not detected in the solution, which showed high separation factors (the ratio between the target peptide recovery and the matrix peptide recoveries) as compared to IEF using EMP [30]. These observations above clearly demonstrate a potential for selective isolation of target peptides from matrix peptides by EME based on differences in isoelectric point.…”

“…Differences in isoelectric point (pI) have been widely used for the separation of ampholytic compounds such as amino acids, peptides, and proteins [29][30][31][32][33][34]. In one example, the isolation of amino acids by iso-electric focusing (IEF) using an electromembrane type process (EMP) was reported [30].…”

“…In one example, the isolation of amino acids by iso-electric focusing (IEF) using an electromembrane type process (EMP) was reported [30]. At pH 8 (above the pI value of glutamic acid (GLU), iso-electric separation of GLU from GLU-lysine (LYS) mixture was achieved with a separation factor of approximately 9 (the recovery ratio between GLU and LYS), and the absolute recovery for GLU was 85%.…”

Electro-membrane processes for organic acid recovery

“…Kumar et al used an electro-membrane reactor where ion substitution and separation of acids occurred simultaneously for the recovery of glutamic acid 115 and amino acid. 166 Their studies resulted in a high recovery of glutamic acid and amino acid close to 96% and 85%, respectively. Meanwhile, Liu et al 167 investigated the potential of an integrated EDBM and biochemical process named the microbial electrodialysis and chemical-production cell (MEDCC) to produce malic acid.…”

Electro‐membrane reactor for the conversion of lysine monohydrochloride to lysine by in situ ion substitution and separation

“…In addition, the flux and recovery of amino acids decrease with increased process time because there is a chance of biomolecules (amino acids) deposition onto the membrane surface due to the hydrophobic nature of the membrane . Electrodialysis has been applied for the separation of different amino acids such as l‐tryptophan from crystallization wastewater, proline and tyrosine from amino acid mixtures, and the separation of lysine, methionine and glutamic acid from their mixture …”